hope this helps :)
Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Rhode Island Hospital
APC Blding, Flr 12, Rm 211
593 Eddy Street
Providence, RI 02903
lab: 401-444-3246
fax: 401-444-8514
nhe...@lifespan.org
-Original
Hazel Horn asks about the ATPase reaction:
Years ago we had problem with our ATPase reaction on F/S of muscle biopsies. We
discovered that in keeping the substrate at minus 20oC in a freezer, and
getting it out and letting it warm up to room temperature before weighing a
small amount out for
I do not know what level of depth this paper needs to be, but these are good
background articles.
http://ergo.human.cornell.edu/AHProjects/Hospital_Ergonomics/lab_radiology.pdf
http://www.uos.harvard.edu/ehs/ih/labergo_microtome.shtmlhttp://www.d.umn.edu/ehso/ergonomics/microtomy.html
The ASCP HTL cert. eligibility criteria listed by BOC is a bachelors.
http://www.ascp.org/Board-of-Certification/GetCertified#tabs-1Not sure if there
is an online available, check the programs listed on NSH, nsh.org , but if you
go into a program with a bachelor's with specific biological
I would also be interested if anyone is using this method and their results. I
have printed off the article and shared the information with our supervising
pathologist. I have found dermal needle rollers on line but wonder if the
maximum 2 mm length will do the job of perforating the fat. Also
Hopefully a hard tissue guru is out there and can help with this.
A PI here is asking about doing a Goldner's stain on mouse femur. I'm not set
up to cut sections of undecalcified bone so my questions are:
1. can this stain be done successfully on decalcified bone?
2. if so, what type of decal
Hi Histonetters!
I hope everyone is having a great day and enjoying some free lunches
celebrating histotechnology professionals day!
I have a new position I would like to tell you about.
Territory Sales Rep Histology-Southern NJ
RELIA is working with a growing lab located in Southern NJ. They
If anyone has access to the article, I would like to get a copy if you don't
mind. thanks
Debbie Siena
800.442.3573 ext. 229 | www.statlab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Does anyone know if bone decalcified in Immunocal is ok to do Her 2 Neu
FISH? Thanks Histonetters! :)
-Bharti Parihar
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As far as i know, osteoid will not show up on a decalcified section.
On Thu, Mar 8, 2012 at 6:00 PM, Grantham, Andrea L - (algranth)
algra...@email.arizona.edu wrote:
Hopefully a hard tissue guru is out there and can help with this.
A PI here is asking about doing a Goldner's stain on mouse
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http://jadehurtz.com/pisces.php?nufugmailID=701
Whod you give the baggage to?Nobody. kodiak adelia
Thu, 8 Mar 2012 19:58:14
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Histonet
Hi Andi,
Yes, you can stain and differentiate osteoid using decalcified bone
sections. You can see osteoid after staining with any trichrome technique,
HE or Toluidine blue. The structure does not disappear with
decalcification. However, do not expect the Goldner’s stain to mimic the
staining
If the path does not want to use Davidsons and still wants the acetic acid,
perhaps Carnoys, Bouins, Methacarn?
Nick Madary, HT/HTL(ASCP)QIHC
George Washington University
Pathology Core Laboratory
Ross Hall, Room 706
23rd and I Street NW
Washington D.C. 20037
202.994.8916
pat...@gwumc.edu
@ Hazel Horn...sorry about not attaching the procedure :/
Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Rhode Island Hospital
APC Blding, Flr 12, Rm 211
593 Eddy Street
Providence, RI 02903
lab: 401-444-3246
fax:
well...looks like I don't know how to attach things so they will post on
histonet...Hazel Horn I sent my procedure to your email too :)
Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Rhode Island Hospital
APC Blding, Flr 12,
Hi
I am looking for help with a cassette printing problem that we are
experiencing. We use the Thermo Histo Screen cassettes and the Surgipath VCP
2001 cassette printer. Case numbers are quite smudged or missing completely
when the batch has finished processing. This is only happening to
Andi,
Your PI has requested the Goldner's trichrome stain because it provides a stark
contrast between the green (light-green SF yellowish) stained mineralized
component of bone and the red (acid fuchsin-ponceau) stained newly formed
unmineralized bone-like dense collagen fibers (osteoid)
This is an issue with the cassettes not your printer or ribbons.
If you take a straight edge and put it up against the edge of the cassette you
will see that he cassette is concaving causing the styles to loose contact with
the cassette.
It is more of a problem at the middle top edge of the
Hello Histonetters! I am new to staining and can't seem to get good HE
stains from deplacticized undecalcified bone with an implant. It seems as
though the hematoxylin is staining too dark. Any feedback would be much
appreciated!
The protocol I am using is for after the deplasticization step
Merissa,
You should very easily be able to get a nice looking HE from deplasticized
undemineralized bone sections. In fact, try the protocol that I routinely use
for my MMA embedded specimens:
Deplastification x 3 changes each for 15-30 minutes @ 55-60C w/ gentle dip and
dunk at half way mark
20 matches
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