Does anyone know if there is an average percentage that a histology lab should
expect in deeper/recut requests? I am trying to establish if our pathologists
order more than the norm or if I have a quality issue unfolding!!
Also, do most labs use 2 controls for their pneumo staining, a routine
For the biopsies that routinely have levels taken, our pathologists would
prefer to order deepers rather than have a possibly diagnostic piece of the
tissue trimmed away before obtaining a section. Yours may feel the same way.
With regard to the controls, our pathologists will specify which
Hi Everyone:
I want to thank everyone who responded to my inquiry regarding marking
pens a while ago.
I had a problem with the Securline Marker2/Superfrost pens fading from
my tissue cassettes.
All three of the pens that I tried: StatLab, Leica, and the Histotec
brands worked really well.
That could be a very difficult to solve issue in some labs. It is entirely
dependent on the pathologists and how confident they feel about their
individual diagnostic abilities and the complexity of some cases.
Some pathologists request more deepers than others and you cannot use the
norm of
Hi again all:
Lately, I have had problems with my Luxol Fast Blue myelin stain not
being nearly as dark as it should.
I am having my oven checked out because I have had problems with it
holding a high enough temperature (at least 60C).
This seems to one factor in getting the stain to
The only thing I think now refers to the age of your solution. If you prepare
it yourself or if bought and near the expiration date probably that could be
another cause of the problem.
René J.
From: Tim Wheelock twheel...@mclean.harvard.edu
To:
Can anyone tell me a company that will test airflow velocity of fume hoods?
Close to or in NC.
Kelly
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True, if you solution is old it may not give the blue color that you want.
Are you doing the overnite procedure? I have found this to be the best. If your
oven isn't holding at 60º C you could use a waterbath.
Andi G
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Hi everyone,
I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC
substrates.
I love DAKO, but their kits and reagents are expensive.
Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies
and AEC substrate kits.
I do all my IHC manually by hand.
Hi Histonetters!!
How are you doing today? Are you gearing up for a fun 4th of July or
getting ready for your well- deserved summer vacation? I wanted to drop you
a quick line to tell you about some especially great opportunities that I am
working on. So grab a cool drink and find some shade
Patrick,
Vector, Lab Vision (Thermo), Jackson Labs, Scytek Labs, all have much less
expensive reagents than Dako. Scytek Labs supplies re-badged reagents for many,
many companies and has a large selection of reagents.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Biocare is another alternative as well.
Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com
-Original message-
From: Morken, Timothy timothy.mor...@ucsfmedctr.org
Date: Wed, 27 Jun 2012 11:00:40 -0500
To: Lewis, Patrick
Luxol fast blue staining is dependent on the polarity of the solvent. Weak
staining can occur if the solvent becomes too polar, i.e. if sections are taken
down to water rather than 95% alcohol prior to putting in the staining
solution. Also check the amount of water in the ethanol or methanol
We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph.
Kit or the DAKO DAB plus kit.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From:
When I request deeper cuts of a paraffin block, I'm usually looking
for a focal lesion that didn't show up in the first section. I don't
usually order a recut because of a crappy slide, knowing that what
I'll get is another crappy slide. I'd actually prefer to write a very
brief note explaining
Hi Guys,
I'm taking apart my AO860 and have a couple issues. First is trying to pull
apart the central shaft that the microtome lowers and raises on. I can get the
primary parts away from each other, and pull off the gears associated with the
handle, but the gear on the central shaft is a
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