Hi all,
A question from my student: antigen retrieval when using frozens for
immunofluorescence -yes or no?
The protocol suggested by the antibody company lists the retrieval as an
option. I am used to do retrieval on paraffin sections, but not on cryo
sections.
Hit me with your opinions on
I assume you are talking about HT(ASCP) Registered techs. The majority of Mohs
Techs are off-the-street people. Here in FL for Registered Licensed techs,
between $25 - $30 / hour. The off-the-street folks $14 - $18, sometimes as
low as $12.00 / hr.
Yours,
Dave
Usually not.
Fixation cross-links the proteins, which can mask the epitope (=antibody
binding site of the antigen). So antigen retrieval breaks the fixative
cross-links, exposing the epitope.
If there's no fixation, there's no cross-links, so the epitope is usually
exposed and available to
Looking for the same
Ian
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carlos Hernandez
Sent: Tuesday, July 03, 2012 11:34 PM
To: Histonet
Subject: [Histonet] Immuno Controls
Hi All!
I was wondering if
Hi Histonetters,
Does anybody has experience in making your own HP control tissue? I am tired of
using positive biopsies of patients since they are always almost finished. I
have heard of a procedure on making HP controls but I am not exactly sure how
it is done.
I have tried the
It may be hard to find someone to help you in the States. Doing that is against
regulations. We must use human tissue with a natural infection I we are going
to be using it as a control for human medical testing. That being said, you
are right, your hp is washing out of the tissue.
My
Hi Willem,
H. pylori needs the low pH of the stomach to survive and grow. It won't
naturally be in lung tissue. When looking for HP, it's found on the mucosa
and gastric pits of the stomach. Since you're looking for it in a specific
place, I think putting it in lung is a bad idea.
Your best
Hello Histonet -
Has anyone had any success staining with PCNA sc-7907 on frozen,
paraformaldehyde-fixed tissue? I am experiencing a lot of background, and
so far diluting down to 1:5000 hasn't helped at all. Any advice would be
very much appreciated!
dilute antibody with %1 BSA.. and use %1 BSA for serum blocking...
On Wed, Jul 4, 2012 at 10:59 PM, Mehmet Fatih BOZKURT fbozk...@gmail.comwrote:
dilute antibody with %1 BSA.. and use %1 BSA for serum blocking...
On Wed, Jul 4, 2012 at 10:21 PM, E Wolfe ewolfe...@gmail.com wrote:
Hello
http://newmodeluk.com/time.php?eight207.jpg
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