Xylene will penetrate nitrile gloves within minutes, so it's good practice to
change gloves when they're wetted with xylene.
You can check this yourself by sniffing your hands after pulling off gloves
contaminated with xylene, you'll know if a 'considerable' amount of xylene has
come through.
--
Thanks all for many useful and helpful suggestions
and interesting anecdotes.
E. Wayne Johnson, DVM
Enruikang AgTech
MOA Feed Industry Centre
China Agricultural University
Beijing
On 8/22/2012 5:27 AM, David A. Wright wrote:
Hi Wayne& Histonet
My guess is that Wayne's crystals are Calciu
Hi Wayne & Histonet
My guess is that Wayne's crystals are Calcium phosphate - the calcium from the
hard tap water, as described, and phosphate from phosphate buffer (sodium or
potassium) in the solution immediately preceding the tapwater rinse. The
variation in crystal deposition would then be
Pam
I hand coverslip and have found in time most of the nitrile gloves break down
and get real soft. However, by accident our purchasing department ordered
SemperSure Nitrile gloves (tested for use with chemothepary drugs) and these
are the only gloves that I found that don't get soft and brea
We use nitrile when handling everything except acetone - then we use latex.
Tresa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richardson, Pam
K
Sent: Tuesday, August 21, 2012 1:53 PM
To: 'histonet@lis
We are beginning a retrograde tracing study in rats. We injected DiD
into the rats and in 4 weeks we will perfuse and harvest the brainstems.
My question is, is vibratome sectioning better than cryostat sectioning?
The literature that accompanied the dye said either is an option, but
some have
Hi Pam:
I use nitrile gloves that we get from our Central Supply Room when
handling xylene and cover-slipping by hand.
They are Kimberly-Clark Sterling Nitrile Powder-Free Exam Gloves.
I use just a single glove rather than double-gloving, and that seems to
do the trick.
The glove usually does
For ordinary applications like manual staining, nitrile gloves work fine. I
don't double glove, just change gloves ad lib.
Jerry
> From: pkric...@gundluth.org
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 21 Aug 2012 19:52:50 +
> Subject: [Histonet] Gloves
>
> Hi, what type of gl
We use baskets here. They get them from office depot. Put each case in one
basket and then put on a cart to deliver to the grosser. For big specimens
that don't fit we just set the container on the grossing table.
Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
The best type is the one used to handle paint removers ("paint thinners").
René J.
From: "Richardson, Pam K"
To: "'histonet@lists.utsouthwestern.edu'"
Sent: Tuesday, August 21, 2012 3:52 PM
Subject: [Histonet] Gloves
Hi, what type of gloves are you wearing whe
Hi, what type of gloves are you wearing when handling xylene? Do you double
glove?
Cordially,
Pam ~
Pam Richardson
Clinical Manager
Gundersen Lutheran Laboratory
Email: pkric...@gundluth.org
Phone: 608 775-4133
Fax: 608 775-6136
Interdepartmental Mail Stop: H04-007
Regardless of what you end doing, NEVER "touch" anything twice to return to the
same container it came.
The idea is that every time you "touch" a specimen you "add value to it".
This means that the specimen should "fall into a chain of work", into a
"forward flow" that makes the specimen one step
Hello my fellow 'netters!
We have another bunch of openings--
Routine bench--
Day, afternoon and night shift -- all over the country and registered and
non-registered openings are available.
Supervisor-
Several openings includind a night shift position
Manager-
One big j
Hello HistonettersI am wondering if anyone can direct me to some good training
courses or sources for IT training, but specifically in areas that would be of
good use for LIS. I have taken some health informatics courses with SQL, some
LOINC,and EMR topics and I am looking to get into these s
My facility currently takes specimens out of plastic bags, accessions the case,
and then stuffs the specimens back into the plastic bags. One of the residents
mentioned that other places put the specimens into plastic bins for the
grossers. I have been asked to evaluate the feasability of this
"Johnson & Johnson just a few days ago announced that they're going to take
formaldehyde out of baby shampoo!"
No wonder I can't get my baby's hair squeeky clean anymore! How much
formalin do I need to add to replace the formalin they took out?
Sincerely?
Jay A. Lundgren, M.S., HTL (ASCP)
___
Obviously, it's your water, if you don't see the crystals after
changing your water. If the acidic pH of the Milli-q water is leaching out
your eosin, then adjust the pH of the water to 7.0 before using it to rinse
your slides.
Sincerely,
Hi Wayne, I had lots of problems with round irregular "crystals", but have
greatly improved my slides by limiting baking at 57 degrees to 1/2 hour. We use
a paraffin with plastic in the mix, and I think the plastic globs up under some
conditions, like no or not enough xylene to dissolve it out.
we are using a Sakura DRS2000 and we are 3 x 3 minutes in xylene and we have
been keeping it fresh. The staining is good now but we still see the
crystals.
If it were paraffin we should see unstained spots on the slide I think.
I have gone to an aqueous 1% HCl today after hematoxylin for regre
Here is a notorious case from my days working in a Ft. Lauderdale suburban
hospital. Along with Bill's experiences we took formalin out of the OR after
this was reported, but it wasn't a requirement as far as I remember.
http://www.nytimes.com/1985/03/10/us/one-death-many-questions-in-miami.html
We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is
responsible for performing a wide variety of histotechnological testing
including, but not limited to, microtomy, cryotomy, complex special stains, IF,
IHC, enzyme histochemistry and electron microscopy. The potential e
We never had a problem until they changed AB's on us. We sent them slides
procedures etc to test but in the mean time we tried Cell Marques AB and it was
clean. Problem solved. Now we all need to demand that Ventana switch back to
Cell Marques H pylori so that we do not have to pay for those #@*
I also have not heard of any recent rule changes. Being aware of the formalin
mishap in OR, our hospital has been using for over 20 years, a light blue
tinged 10%NBF that the supplier makes up special. The blue is actually just a
few drops of food coloring that the manufacturer adds, and the bl
I know of no rules, though there may well be. I do know that several
years ago a patient was injected (IV)with formalin in the OR. Results
were NOT good. I know that the OR in the place I was working at the time
would no longer allow for formalin in the OR suite. The tissues were put
in formalin in
All,
What is your favorite Renal Cell Carcinoma Antibody?
I'm using the Leica Bond stainer with Leica's detection & am looking for the
best fit.
In other words, the antibody that I am using is working, but I want it to look
better.
Any responses would be greatly appreciated.
Glen D
Debra and others:
All of our specimens are sent fresh because surgery says formalin cannot be in
the operating rooms. I'm told it comes from AORN, which is the association for
operating room nurses.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children'
Our unit of measure is billable test. But, my director adds in extra (not sure
how) for manual vs. automation in the clinical lab.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen
- SMMC-S
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