[Histonet] Reliable Histology Team Member to embed and cut for you.

Thanks for your interest in this matter and for the ability of others to advertise or not, but I'm not sure why this is any concern of yours. You are the ONLY poster who has responded in a negative way. I take what I do very seriously and would gladly pay for advertising on this forum. If we do

[Histonet] Local service company for cryocut 1800 in San Diego area?

Hi Guys Can anyone recommend a local service company for the cryocut 1800 in the San Diego area. Thanks in advance. Jennifer Jennifer L. Johnson, Ph.D. Staff Scientist The Scripps Research Institute ___ Histonet mailing list Histonet@lists.utsouthwes

RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

I agree with Rene and Tom. You have learned the skills and need to be in a place where you are happy and in an environment that fosters development, cooperation, and innovation. What you have described is the opposite situation. Hopefully you can find a better home in your area, not a "my way o

[Histonet] duel stains

Hi Netters,  Just curioushas anyone stopped doing dual IHC (ie. PIN4) du e to the charging changes?  We are going to start doing the  three stains separately.  Any opiniions? Linda Hartman HT(ASCP) MNMC  ___ Histonet mailing list Histonet@l

[Histonet] Acid Phosphatase Reaction

Hello to all Histologists, Perhaps someone can help me. At our institution we primarily use electron microscopy for slides with toluidine blue, and grids for TEM. The students here also do some immunostaining on their tissues. We have a student though who would like to try using the Acid Pho

Re: [Histonet] Reliable Histology Team Member to embed and cut for you.

Is advertising allowed on Histonet now, or not? When I brought up this issue before, people responded, "Oh, if it's just a tech looking for a job once in a while..." etc. This is at least the third time this COMPANY has advertised on here. This forum is not meant for marketing. I'm sure

RE: [Histonet] IHC new reagent lots

We run a control(s) first before using it on patient samples. We compare that slide to the same/similar control(s) run with the current lot. Our director reviews both slide(s) and if comparable or better, signs off on the new lot. -Original Message- From: histonet-boun...@lists.utsouthw

Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

I haven't mentioned that the hospital where I work has been facing financial problems and my job has been threatened a couple of times. My work hours have been reduced from 40 hours a week to 32 since Feb and seems that they have no intentions of increasing them again plus I am underpaid with no be

[Histonet] IHC new reagent lots

I'm in a new position and have to get our IHC paperwork CAP ready. I'm not sure about something. When you put a new antibody dispenser (with a different lot # than previously used) on the IHC stainer, what QC needs to be done? Can the control run with a patient test serve as your QC, or does it

[Histonet] RE: positive control desperately needed for IDH1 R132H antibody

Hi Johanna, I think it is going to be hard to find brain tissue that has already worked up for that antibody. You probably have to work up the antibody yourself. But if it is this antibody: http://www.ncbi.nlm.nih.gov/pubmed/19798509 It ought to work on 70% of astrocytomas. Abcam sells co

[Histonet] positive control desperately needed for IDH1 R132H antibody

Hi, we need to validate our IDH1 R132H antibody on a known positive tissue. We are a small lab and so far we could not get hold of a proven positive specimen. We did not have any luck contacting the antibody's manufacturer either. Could someone name a source from where we could get some sections

RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

I was once worked at a place that had this sign on the wall that everyone saw as you entered the training room. "When you think you are through learning, you are through here" Blows my mind when I run into people that refuse to learn anything current. Tom Podawiltz HT (ASCP) Histology S

[Histonet] Reliable Histology Team Member to embed and cut for you.

Hello histonetters, I'm an experienced histology technician based in the south. I am available to help keep the cogs turning smoothly so to speak in your lab. I contract my services through my business Histocare. What I do: I primarily work within the South (specifically TN, AL, AR, MS, GA

Re: [Histonet] Ventana temp veriifer slides

I'm doing the even one quarter and the odds the next also. I number the slides in Sharpie to cprrespond to the heat pad # after they are finished and take a photo of them for documentation, then toss the slides. es...I've decided to do the even's 1 quarter and the odds the next quarter (becau

[Histonet] Ventana temp veriifer slides

Just curious what everyone else does...I've decided to do the even's 1 quarter and the odds the next quarter (because of cost)...do you just run them, document it and throw them away? Or does anyone make a copy of them and save in the QC book? Thanks! _

Re: [Histonet] Flourescent mounting medium

Hi Candice, Not sure why you are using this. Have you tried any of the commercial preps? I like the one called Fluor-glo from Valley Scientific. www.Valley-Scientific.com Loralei On Mon, Oct 1, 2012 at 7:40 AM, Candice Smoots wrote: > Hi histonetters, > > I was hoping that I can get some infor

[Histonet] Flourescent mounting medium

Hi histonetters,   I was hoping that I can get some information on this fluroscent mounting medium that we are trying to sue in our lab. It is called Polyvinyl alcohol mounting mrdium with DABCO.   If you have ever used this... please send me a protocol fro it. We are having trouble getting it t