Re: [Histonet] CD11b

Me too!!! C On 11/6/2012 3:44 PM, Bell, Pat wrote: Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat

[Histonet] CD11b

Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Onc

Re: [Histonet] How to fix frozen section?

i use 1:1 Acetone : alcohol. On Nov 6, 2012, at 2:41 PM, Amy Lee wrote: > Hello, > > I will receive some frozen tissue sections that are not fixed. I rarely do > IHC on frozen sections. I searched some articles about fixation. Some use > 50/50 of acetone/alcohol and some use only alcohol. Co

Re: [Histonet] How to fix frozen section?

I used acetone René J. From: Amy Lee To: histonet Sent: Tuesday, November 6, 2012 2:41 PM Subject: [Histonet] How to fix frozen section? Hello,   I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched som

[Histonet] Re: Histonet Digest, Vol 108, Issue 8

Leica Cryostat CM1850 UV for me! Used them for more then 20 years and still prefer them.:) Madeleine Huey On Tue, Nov 6, 2012 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit

Re: [Histonet] Devasting news on 88305TC component--Where the $18 came from

Well in this article it does have differences, especially when talking about usage of technology,, but non the less it does expose our state of being within Anatomic Pathology,, ,, what are peoples thought on this Sent from my iPad On Nov 6, 2012, at 12:21 PM, "Lester Raff MD" wrote: > Sent:

RE: [Histonet] How to fix frozen section?

Amy, We use ice-cold acetone/ethanol (3:1) for 10'...works for all of our antigens so far. Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message-

[Histonet] Histologist

Busy Derm Practice looking for an experienced Histologist to run Derm Path lab. Full or Part time. Must be able to perform all routine histology duties soley. Embedding, grossing, accession, coverslipping, microtonomy, record keeping, H&E stain and special stains. Candidate needs to be familiar wit

RE: [Histonet] How to fix frozen section?

It depends upon what marker you're staining for. We fix our renal biopsies for C4d in acetone for 10". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet

[Histonet] How to fix frozen section?

Hello,   I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me?     Thanks,   Amy ___

FW: [Histonet] Devasting news on 88305TC component--Where the $18 came from

Sent: Tuesday, November 06, 2012 12:35 PM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Devasting news on 88305TC component--Where the $18 came from The $18.00 figure came from an article published in Archives of Pathology and Lab Medicine: Pathology Economic Model Tool

[Histonet] SC Society of Histotechnology Meeting This Weekend

Good afternoon Everyone This is just to let you know the SCSHT Meeting will be this weekend November 9-10, 2012 at the beachfront Landmark Resort in Myrtle Beach, SC. 7contact hours available and workshops include "Lean Workflow in Histology: Error Reduction" "Tissue Processing from the B

[Histonet] Job Opportunity!!!

Medical Center Hospital is a premier center and we currently have opening for Histotechnicain/Histotechnologist in our Department. This is a full time opportunity that will rotate through all area of histology routine procedures, special stains and IHC.Please if interested send your resume to m

RE: [Histonet] Looking at cryostats

Leica! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY

RE: [Histonet] Looking at cryostats

Thermo NX70 is very nice. In fact everyone fights to use it rather than the old CM3050 we have... We use them for frozen sections for kidney, skin IF and muscle histochemistry. Lots and lots of frozens... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pa

RE: [Histonet] Looking at cryostats

Leica Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci

Fw: [Histonet] Cytology in Jasper, Indiana

Good Morning Histonetters, We are currently looking for a registered Cytotechnologist to work in a Regional hospital doing all aspects of cytology. The position is full time requiring someone with a clinical Cytology background with processing, screening, assisting on FNA's and learning Cervis

histonet@lists.utsouthwestern.edu

That is quite acceptable cost. Under separate cover I am sending my costs. René J. From: Cheryl To: "histonet@lists.utsouthwestern.edu" Sent: Monday, November 5, 2012 10:02 PM Subject: [Histonet] cost per H&E Depending on how I dice up labor and materials, I

RE: [Histonet] Looking at cryostats

The CM3050s is the best in my opinion for research or heavy duty frozen sectioning and it can work with the Cryo Jane system or similar if needed. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf O

Re: [Histonet] Looking at cryostats

Not really! For me the Leica is the best. René J. From: "kgrob...@rci.rutgers.edu" To: histonet Sent: Tuesday, November 6, 2012 9:25 AM Subject: [Histonet] Looking at cryostats What's good out there these days?  I am currently looking at the Leica CM3050; are

Subject: [Histonet] Background staining on H pylori

Hey Deloris, I would have the dispensing nozzles on the XT checked, could be it is not getting rinsed properly. Had that issue with my Ultra not to long ago. No warnings or errors showed up, just a clogged nozzle tip. Tim -Original Message- From: histonet-boun...@lists.utsouthweste

[Histonet] Looking at cryostats

What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University o

RE: [Histonet] Background staining on H pylori

Deloris, We recently had what we think was a contaminated dispenser of HP from VMS. It had brown particulate matter scattered over the slide which the docs said was not bugs. Received a new dispenser from VMS and have had no problem since. Linda A. Sebree University of Wisconsin Hospital &

RE: AW: [Histonet] overfixation with formalin C.Fox pdf article

Thanks Joelle. I took it from your block. Regards, José Luis Dr. José Luis Palazón Fernández Instituto de Investigaciones Científicas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedr