RE: [Histonet] Alcian Blue

2012-12-07 Thread Susan.Walzer
We've always used 30 minutes and it has never failed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: histonet@lists.utsouthwestern.edu Subject:

RE: [Histonet] Alcian Blue

2012-12-07 Thread Lynette Pavelich
30 here. Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of susan.wal...@hcahealthcare.com [susan.wal...@hcahealthcare.com] Sent: Friday, December 07, 2012 3:04 AM To: sa...@hotmail.ca;

Re: [Histonet] Alcian Blue

2012-12-07 Thread Rene J Buesa
If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them in house check the preparation date and prepare a fresh solution. The general

Re: [Histonet] Paraformaldehyde Solution Recipe for Perfusion

2012-12-07 Thread Geoff
What issues are you having? That might help the list diagnose the problem. Geoff On 12/6/2012 3:43 PM, Andrew Coleman wrote: Hi all, We are performing transcardial perfusions in rats using paraformaldehyde in 0.1M potassium phosphate buffer. Can anyone think of any issues that would be

[Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

2012-12-07 Thread Tim Wheelock
Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol

[Histonet] question(s)

2012-12-07 Thread Webb, Dorothy L
How does everyone handle storing extra paraffin sections that are cut as a standard on certain protocols, such as prostate needle bx's? We currently place them on a slide and save until the case is signed out. I am concerned with the amount of waste and cost with the way we are doing it and

[Histonet] Message 2: dako her2

2012-12-07 Thread M. Kap
Check www.cqpath.com for more info Verstuurd vanaf mijn iPad Op 7 dec. 2012 om 19:03 heeft histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu het volgende geschreven: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To

RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

2012-12-07 Thread Cynthia Pyse
Yes you are. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] question(s)

2012-12-07 Thread Rene J Buesa
The sectioning protocols/sequences are determined by the pathologists and what they think is required to make diagnoses. We also stored the slides with extras sections until the case is signed and get an additional OK from the signing pathologist before disposing of the unused sections. I would