We've always used 30 minutes and it has never failed. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Sheila Adey Sent: Thursday, December 06, 2012 8:58 PM To: [email protected] Subject: [Histonet] Alcian Blue
Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
