If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol. René J.
From: Sheila Adey <[email protected]> To: "[email protected]" <[email protected]> Sent: Thursday, December 6, 2012 8:58 PM Subject: [Histonet] Alcian Blue Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
