Re: [Histonet] Chromic Acid for Cleaning Glass

I use to clean the glassware with the usual household detergents, then washing them carefully with distilled water. I pay attention on degreasing the slides and the cover glasses. Greasy traces are responsible of the detachment of the specimen slices attached on them. I use to clean them with thi

Re: [Histonet] Chromic Acid for Cleaning Glass

Household bleach is a reasonable alternative for acid cleaning glassware. Jackie -Original Message- From: Pablo Sanchez-Quinteiro To: Histonet Sent: Thu, Feb 14, 2013 3:37 am Subject: [Histonet] Chromic Acid for Cleaning Glass Dear Listers, I think that the use of Chromic Acid for c

[Histonet] RE: "Vacuole" appearance in lymph Nodes

Ken, Are the Microtomists being over enthusiastic with the block "trimming" or "facing"? This is more prone to happen if the block is cold (ie harder) during trimming. I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes

RE: [Histonet] standard protocol

Hi Kay, Who was the idiot who sent this to you? Why bother cutting the sections if they will float off in the xylene, you might as well just send them blank slides. The companies are willing to spend millions of dollars on the development but couldn't be bothered seeking professional advice be

[Histonet] FW: CD4 CD8 Zinc Tris PE staining

Hello Patsy, You wrote: Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8. I found some papers that look impressive. I presume you mean the Beckstead zinc TRIS fixative that is formalin free? It should work with murine or rat tissu

RE: [Histonet] H&E Destain protocol

Not if it is just from processing. Eosin from processing will not affect any IHC or Special stains. If it was H&E stained you just need to take back through Xylene, alcohol to water. The alcohol will remove some of the eosin, and the IHC process will not be affected by the stains. It will simpl

[Histonet] Monitor & Key Board Mounts

I need to order Monitor & Keyboard Mounts for our custom made Grossing Stations. Need suggestions the standard ones that com with Gross Stations are very expensive. Helena Howe ProMedica Laboratories _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIAL

[Histonet] zinc salt fixed mouse tissue PE stained for CD4/cd8

Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8 I found some papers that look impressive. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 rueggihccons

[Histonet] H&E Destain protocol

Hello Histonet, I have samples that were stained during fixation with Eosin. First question, since I will do IHC on them, do I need to get rid of the eosin like you do with an already H&E stained slide? If so, that brings me to my second question, does anyone have a protocol that are willing t

Re: [Histonet] FISH baking and dewaxing

Hi Ronnie, FISH pretreatment can be harsh and that might explain the long baking (don't want the tissue to fall off). On the other hand, I've put a slide in the oven for 5 minutes and had the tissue stay on in a rush. I think the dewaxing can be shortened without affecting the FISH signals. Our

[Histonet] FISH baking and dewaxing

Can someone please explain why paraffin-embedded slides for FISH need to be baked for so long and have extensive dewaxing in xylenes and subsequent alcohol pretreatment? Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 changes of xylene 15 min each, and 100% ethanol 15 min

Re: [Histonet] Slide Mate printers

We bought the Print Mate for Cassettes and Slide Mate for slides over two years ago.  We use the Thermo Colorfrost slides and they work well.  We had issues in t he beginning and it was very difficult.  Thermo hung in there and made sure we were happy and got things working well.  We are very

RE: [Histonet] tissue processor remote alarm systems

I have a Sensaphone unit. It can handle four different instruments. When it calls it will give you the phone number it's calling from, the sensor (instrument) number, and the 'alert condition' (there are several...for example 1 is power failure, etc.) Any other questions about it I'm happy to hel

[Histonet] Slide Mate printers

We have the slide mate printers. We have the best luck with Item# 12 550 15 Slide Superfrost White (there are color slides also). I have found that you have to remove excess used printer ribbon from the unit at least once a week. One day all my prefixes disappeared from my slides, investigated t

[Histonet] RE: General Data

We use a full PC mounted under the counter and a touchpad mounted on an arm that can be positioned just about anyplace. The printer is mounted on a small shelf below the counter. Small scanner mounted to a desktop stand that can be moved wherever the cutter wants. Tom McNemar, HT(ASCP) Histo

RE: [Histonet] CD31 problewms

I think the CD31 Ab from Dianova is what works best for most people on FFPE mouse tissue. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message

RE: [Histonet] RE: SlideMate Printers

We had a demo of the new Sakura Slide printer and it is awesome compared to the Slide Mate. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax ===

[Histonet] RE: SlideMate Printers

Hi Lori, We have been trying to get the SlideMate/ Printmate system working in our lab for close to a year now. Our experience with the SlideMates has been dismal. Our quality with the Slidemates is minimally adequate, with a lot of 'misprints'. We didn't get the best print with their Colorfro

[Histonet] Open Position

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Re: [Histonet] CD31 problewms

If you have not changed the Ab provider nor any step on your protocol, check the Ig concentration in the lot you are using now compared with the lot you used to determine the Ab dilution. If the Ig is less concentrated now, you may need to increase the concentration (reduce the dilution). René J

[Histonet] CD31 problewms

We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With Decloak

[Histonet] RE: General Data

Nancy, what kind of system are you going to use - a barcoded tracking system to scan blocks at the microtome, or do you have to type in case info to get the labels? Tim Morken UCSF Pathology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lis

[Histonet] "Vacuole" appearance in lymph Nodes

Hi All, I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already. Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen. They have different patterns section to section so

[Histonet] General Data

Good Morning- We are currently using General Data for cassette labeling - very happy with it. The next phase is implementing slide labeling. I would appreciate knowing what kind of setup people are using at the microtome - laptop? touchpad? Full computer? Is the arm off of the microtome or out f

Re: [Histonet] standard protocol

"Standard" protocol is a very "encompassing" word and implies that it is "standard" for all labs and there is no such standard. Each lab has its own (although sometimes adhering to some general consensus) "standard" protocol, so probably that pharmaceutical company is referring to their "standar

RE: [Histonet] standard protocol

Ask the pharmaceutical company for detailed instructions, not vague references. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histopatty Sent: Thursday, February 14, 2013 9:02 AM To: histonet@lists.utsout

[Histonet] standard protocol

We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wonder

[Histonet] tissue processor remote alarm systems

I'd like to hear from labs that use a remote messaging system for their VIP5 tissue processors that are left unattended overnight. One I saw on old histonet posts is Sensaphone. I am wondering what kind of information you can get from the system. Does it just say there is a problem but no detai

Re: [Histonet] bone marrow specimens

I fix in NBF at pH7 exactly and decalcify with EDTA René J. From: Clare Thornton To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, February 14, 2013 9:22 AM Subject: [Histonet] bone marrow specimens What fixative and decal solution is everyone using for bone marrow specimens which will

[Histonet] Technical Coordinator of Histology Opening Near Fort Myers, FL

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[Histonet] RELIA Histology Careers Bulletin 2-14-2013 Happy Valentines Day!

Hi Histonetters!! Happy Valentines' Day!!! I have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! All of these are permanent full time positions and our cli

[Histonet] bone marrow specimens

What fixative and decal solution is everyone using for bone marrow specimens which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists demand a quick turn around time, and are willing to sacrifi

[Histonet] Chromic Acid for Cleaning Glass

Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) ___ Histonet mailing list Histonet@lists