Dear Listers,
I think that the use of Chromic Acid for cleaning the glassware is
currently declined. Do you keep using it? Could you suggest me a
non-comercial alternative?
Thanks in advance,
Pablo (Spain)
___
Histonet mailing list
What fixative and decal solution is everyone using for bone marrow specimens
which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently
using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists
demand a quick turn around time, and are willing to
Hi Histonetters!!
Happy Valentines' Day!!!
I have some great histology opportunities to tell you about.
Please feel free to take a second and peruse the list kind of the way one
peruses an Assortment of chocolates in a heart shaped box!
All of these are permanent full time positions and our
Position: Technical Coordinator of Histology
Schedule: Monday-Friday Day Shift
Location: Fort Myers, FL
IHC experience is a must! Email bran...@alliedsearchpartners.com for a full
job description.
To view a complete list of Allied Search Partners current openings go to:
I fix in NBF at pH7 exactly and decalcify with EDTA
René J.
From: Clare Thornton cthorn...@dahlchase.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, February 14, 2013 9:22 AM
Subject: [Histonet] bone marrow specimens
What fixative and decal solution
I'd like to hear from labs that use a remote messaging system for their VIP5
tissue processors that are left unattended overnight. One I saw on old histonet
posts is Sensaphone.
I am wondering what kind of information you can get from the system. Does it
just say there is a problem but no
We are getting instructions from a pharmaceutical company who needs slides,
with instructions stating sections should be floated per standard protocol,
xylene dipped from 3 minutes and dried for 10 minutes. I'm reaching out to
those of you who routinly deal with research protocols and
Ask the pharmaceutical company for detailed instructions, not vague references.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histopatty
Sent: Thursday, February 14, 2013 9:02 AM
To:
Standard protocol is a very encompassing word and implies that it is
standard for all labs and there is no such standard.
Each lab has its own (although sometimes adhering to some general consensus)
standard protocol, so probably that pharmaceutical company is referring to
their standard
Good Morning-
We are currently using General Data for cassette labeling - very happy with it.
The next phase is implementing slide labeling.
I would appreciate knowing what kind of setup people are using at the microtome
- laptop? touchpad? Full computer? Is the arm off of the microtome or out
Hi All,
I have sent this question to respected colleagues individually so pardon my
redundancy if you have this email already.
Lately we have had random nodes show up with areas that appear to have multiple
vacuoles through-out the specimen. They have different patterns section to
section so
Nancy, what kind of system are you going to use - a barcoded tracking system to
scan blocks at the microtome, or do you have to type in case info to get the
labels?
Tim Morken
UCSF Pathology
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We are having problems getting our CD31 to work well on our NBF fixed mouse
tissue. There is staining but it is very pale.
The CD31 comes from Bio-care and we are are using the IntelliPATH stainer.
We have tried:
- Trypsine 37C 15 min / Trypsine RT 30 min
- Trypsine (1:2 and 1:3)
- With
If you have not changed the Ab provider nor any step on your protocol, check
the Ig concentration in the lot you are using now compared with the lot you
used to determine the Ab dilution. If the Ig is less concentrated now, you may
need to increase the concentration (reduce the dilution).
René
Cook Childrens Medical Center in Ft Worth, TX has an immediate day shift
opening for a full time HT (ASCP).
Interested candidates must apply online at
www.cookchildrens.orghttp://www.cookchildrens.org and meet one of the below
qualifications listed at the online job posting to be considered.
We had a demo of the new Sakura Slide printer and it is awesome compared to the
Slide Mate.
Victor
Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
I think the CD31 Ab from Dianova is what works best for most people on FFPE
mouse tissue.
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
-Original
We use a full PC mounted under the counter and a touchpad mounted on an arm
that can be positioned just about anyplace. The printer is mounted on a small
shelf below the counter. Small scanner mounted to a desktop stand that can be
moved wherever the cutter wants.
Tom McNemar, HT(ASCP)
We have the slide mate printers. We have the best luck with Item# 12
550 15 Slide Superfrost White (there are color slides also).
I have found that you have to remove excess used printer ribbon from the
unit at least once a week. One day all my prefixes disappeared from my
slides, investigated
I have a Sensaphone unit. It can handle four different instruments.
When it calls it will give you the phone number it's calling from, the
sensor (instrument) number, and the 'alert condition' (there are
several...for example 1 is power failure, etc.) Any other questions
about it I'm happy to
We bought the Print Mate for Cassettes and Slide Mate for slides over two years
ago. We use the Thermo Colorfrost slides and they work well. We had issues in
t he beginning and it was very difficult. Thermo hung in there and made sure
we were happy and got things working well. We are very
Can someone please explain why paraffin-embedded slides for FISH need to be
baked for so long and have extensive dewaxing in xylenes and subsequent alcohol
pretreatment?
Our Molecular group bake for 1 hr at 65C, and then treat sections in 3 changes
of xylene 15 min each, and 100% ethanol 15
Hello Histonet,
I have samples that were stained during fixation with Eosin. First question,
since I will do IHC on them, do I need to get rid of the eosin like you do
with an already HE stained slide? If so, that brings me to my second question,
does anyone have a protocol that are willing
Has anyone tried this: zinc salt fixed mouse tissue PE stained for CD4/cd8
I found some papers that look impressive.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
mailto:rueggihcconsultin...@outlook.com
I need to order Monitor Keyboard Mounts for our custom made Grossing
Stations. Need suggestions the standard ones that com with Gross Stations are
very expensive.
Helena Howe
ProMedica Laboratories
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
EMAIL
Not if it is just from processing. Eosin from processing will not affect any
IHC or Special stains. If it was HE stained you just need to take back through
Xylene, alcohol to water. The alcohol will remove some of the eosin, and the
IHC process will not be affected by the stains. It will
Hello Patsy,
You wrote: Has anyone tried this: zinc salt fixed mouse tissue PE stained
for CD4/cd8. I found some papers that look impressive.
I presume you mean the Beckstead zinc TRIS fixative that is formalin free?
It should work with murine or rat
Hi Kay,
Who was the idiot who sent this to you?
Why bother cutting the sections if they will float off in the xylene, you might
as well just send them blank slides.
The companies are willing to spend millions of dollars on the development but
couldn't be bothered seeking professional advice
Ken,
Are the Microtomists being over enthusiastic with the block trimming or
facing?
This is more prone to happen if the block is cold (ie harder) during trimming.
I would recommend after trimming to full-face that a few slower turns of the
microtome wheel at 4-5 microns will clear the holes
Household bleach is a reasonable alternative for acid cleaning glassware.
Jackie
-Original Message-
From: Pablo Sanchez-Quinteiro pablo.sanc...@usc.es
To: Histonet Histonet@lists.utsouthwestern.edu
Sent: Thu, Feb 14, 2013 3:37 am
Subject: [Histonet] Chromic Acid for Cleaning Glass
I use to clean the glassware with the usual household detergents, then washing
them carefully with distilled water.
I pay attention on degreasing the slides and the cover glasses. Greasy traces
are responsible of the detachment of the specimen slices attached on them.
I use to clean them with
31 matches
Mail list logo
