Should be no difference ( no tricks)
Have a look here for some images:
http://www.immunoportal.com/
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson Centre for Age-Related Diseases
School of Biomedical Sciences
King's College London
Guys Campus
London SE1 1UL
Tel.020 7848 6810
fax 020
Hi Histonetters!!
I hope you are gearing up for a fun weekend. I have a new job opportunity
that I am very excited about and want to share with you.
This is one of my best clients. I say this because I have placed a number
of people there and they love it!
Here is the info:
Histology/ Lab
The heating element for the front chamber on my formerly very dependable
Microm AP 280 embedding center has burned out, right in the middle of
teaching histotechniques this semester, and with grad students and me in
need of it (of course!).
I am wondering if anyone has a working unit of this
Register today for the first ever 2 day Carolina Symposium sponsored by the
National Society for Histotechnology, the North Carolina Society of
Histotechnology and the South Carolina Society of Histology Technicians. This
two day collaboration brings the best of the three organizations
I wanted to share our competency spreadsheet that we started at the beginning
of the year.
I'm attaching the histology version. Not sure if attachments work on the
histonet. If not, and you are interested, I'd be glad to share my template
with anyone. It addresses all 6 key points.
Billie
Hello Histo-World,
We are experiencing randomized curling of our thick (40um) frozen mouse
brain sections. This curling occurs in the cortex and hippocampus region
(these are coronal sections) during the counterstain process for Cresyl
Stain (when the tissue is hydrated post alcohol gradient). Is
Hi All,
Someone called me from a nearby Histo laboratory. They have approx 75
plastic block file cabinets that they would like to re-home for free, but new
owner must pay shipping or come get them in Springfield, MO. If anyone is
interested you can contact me at oreillepoint...@msn.com
Does anyone perform this antibody?? We can't get it to work on the Ventana
Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small
size polymer detection kit. I would appreciate any input.
Thanks,
Billie Zimmerman
Augusta State University and Georgia Health Sciences
Are you using the antibody from Dianova??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman,
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject:
Billie,
We use Dianova's antibody and purchase it from Fisher. We've performed this
stain for a little over six months and seem to be having success with the
following protocol:
Dianova's IDH1 R132H Antibody, Diluted 1:100
Ventana Benchmark XT, iView Detection
Standard CC1
37 degrees C
32
We run it on Leica's Bond Max with good results. We use the mouse mAb (Clone
H09) from dianova at a dilution of 1:50 (15' incubation) following low-pH
retrieval.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant
I received a spec sheet from Dianova that has their Ventana XT protocol. It
suggests using CC2 standard retrieval and incubation for 32 minutes with amp.
They give a dilution range of 1:20-1:50. It seems to work well for us although
we are still validating. I hope that helps.
-Original
The differences might be due to decalcification and/or differences in fixation.
I presume you are decalcifying the fish? Are you using EDTA or a formic acid
decalcifier? Are they fixed in 10%NBF (or equivalent) for roughly the same
amount of time as your human samples? Or are you using a
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