Re: [Histonet] QIHC

2013-03-04 Thread Lee Peggy Wenk
Just to let everyone know - the Michigan Society of Histotechnologists have put together a Study Guide/Workbook, geared to help those studying for the QIHC. It is a workbook, where you have to look up the information in books, and write it in the booklet. There are no answers provided (it's not

Re: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS

2013-03-04 Thread Lee Peggy Wenk
Can you surface decal? After facing the block on the microtome, getting a full face, pour some decalcification fluid in the lid of a coplin jar. Place the block faced side down in the decal solution, for about 30 minutes. Rinse the acid off the block with some cool water (don't want acid

Re: [Histonet] Unencased Ameoba Stain

2013-03-04 Thread Lee Peggy Wenk
Getting back a little late (been out of state). If this is Acanthamoeba, then we do a PASH and/or a GMS. These are cysts, and show up nicely with both. The cornea can be PAS positive also, so the Acanthamoeba are a darker pink against a lighter pink, while the GMS is gray/black against the

Re: Re: [Histonet] teeth sectioning

2013-03-04 Thread E. Wayne Johnson 朱稳森
Jack, That sounds really awesome. I did some work with the teeth of sows (female pigs) from specimens collected at slaughter. Those are very difficult to decalcify, and when finished, are likely to have no nuclear detail remaining. Interested to hear what you learn Wayne Johnson Beijing

[Histonet] pH and temperature

2013-03-04 Thread Emily Sours
Hello! Can someone explain why pH is temperature dependent? Really, I'm just worried about pHing 4% paraformaldehyde at about 50 degrees Celsius. If I wait until it cools down to room temp, the para usually precipitates--I assume because the pH is off. I know I could measure the pH at room

[Histonet] KY HT Poisitons

2013-03-04 Thread Hale, Meredith
Please no Recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to pateints with

[Histonet] standards for embedding

2013-03-04 Thread Diana McCaig
I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range

Re: [Histonet] standards for embedding

2013-03-04 Thread Rene J Buesa
Ontario standards are above national and international averages and would be difficult to duplicate for the majority of labs. Your averages are more in accordance with the averages I mention. Please go to http://www.histosearch.com/rene.html to compare with the results of a survey I published.

[Histonet] RELIA Solutions HOT JOB ALERT and a link to my blog post Essential Tips for Working with a Recruiter 3/4/2013

2013-03-04 Thread Pam Barker
Hi Histonetters!! I hope your week is off to a great start!! I know mine is!! I have a couple of new opportunities that I want to share. Also I recently started a blog called Dream Job Diva here is the link to one of my posts if you think you might like to check it out. This has been without a

[Histonet] Jekyll Island, GA

2013-03-04 Thread Zimmerman, Billie
Come to Jekyll Island for the sunrise, sunsets, and CEU's!! Earn up to 15 CEU's with incredible speakers and a chance to network with your peers for the low all inclusive rate of $135. GSH is packing a punch for $135. GSH has planned an educational 40th anniversary celebration with fun, food,

[Histonet] Re: teeth sectioning

2013-03-04 Thread Teri Johnson
This might or might not be helpful, but there was an article written by Janet Maas, Processing the Complete Canine Tooth. It is for paraffin sectioning. JOH, Number 3, Sept 2002, pp. 137-140(4) Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research

[Histonet] Re: pH and temperature

2013-03-04 Thread Emily Sours
Well, I found this on the site below: To make an accurate pH measurement you need to use temperature compensation. Exception is if you measure a pH of about 7. Which makes me feel better. Every other solution I pH is made at room temperature. I don't know why our para precipitates out--it might

RE: [Histonet] Ventana HPV High/Low Replacements?

2013-03-04 Thread Michael Kent
Hi Jennifer, I'd recommend looking at this study and comments on biomarkers in HPV: The Lower Anogenital Squamous Terminology Standardization Project for HPV-Associated Lesions: Background and Consensus Recommendations from the College of American Pathologists and the American Society for

[Histonet] QC Slide retention

2013-03-04 Thread Riesen, Rebecca
How long do you folks retain you Daily QC slides? Is it the same as the diagnostic slides? I know the correct length of time for diagnostic slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily HE or PAP QC slides. Is the retention of the actual

[Histonet] Sponges and Processing

2013-03-04 Thread Laurie Colbert
If there is anyone out there that uses the blue sponges for biopsy specimens, would you please contact me offline? I would like to know what your processing times are. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214

[Histonet] RE: QC Slide retention

2013-03-04 Thread Bernice Frederick
We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723

[Histonet] RE: QC Slide retention

2013-03-04 Thread Weems, Joyce K.
We do the same. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for

[Histonet] Per Diem opportunity near Port Chester, NY

2013-03-04 Thread Brannon Owens
Allied Search Partners is currently working with a large organization with leading edge technologies to find a qualified Histology professional. Location: White Plains/Port Chester, NY area Position title: Histotechnician or Histotechnologist Schedule: Sunday 5am-1:30pm Email

[Histonet] RE: Antibody IDH1

2013-03-04 Thread Sue Hunter
We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell conditioning for 40 minutes, antibody for 32 minutes - no heat. We use Optiview detection system. No amp. Make sure you are using the correct type of brain tissue for a control - most oligodendrogliomas should be positive,

[Histonet] SOP numbering for whole lab

2013-03-04 Thread Cheryl
Help?  What kind of formula do you use when numbering and coding your SOPs for the lab?  Is there an easy way to keep them sorted and in line with all the regulatory bodies to help on inspection days?   Any suggestions welcome--we're starting from scratch.   Cheryl    

[Histonet] Back processing Histogel

2013-03-04 Thread Andrea Hooper
Has anyone back processed something that was both Histogel and FFPE embedded? If so, any hints or problems? Andrea ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Paraffin block archival storage

2013-03-04 Thread Clarence Owens
Does anyone know if there is a CAP or board of health regulation on storing archival paraffin blocks in a walk in morgue cooler. Currently we are storing our archival paraffin blocks in the morgue cooler for the ten year retention CAP regulation. The blocks are at a controlled temperature where