Pretreatments are used to recover bonded antigen sites owing to formalin
linkage. What is the optimum or maximum fixation time for tissues that may
require Immunohistochemistry staining?
IB
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We are looking into the option of neutralizing our formalin waste instead of
having it to be picked up.
If you use Formalin Neutralizer, do you have any pro/con about this product?
Thank you very much.
Lin S. Bustamante, B.S., H.T.(ASCP)
VIBS Histology Laboratory Supervisor
College Of
Hi all,
Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months?
We are going crazy with compression problems and have tried all the usual
solutions (tightening all parts of microtome, changing angles, changing blades,
servicing microtome) and the problem persists. This
We neutralize ours and have no problems with it. I am not sure how much you
use, so I will say it is easiest in smaller batches as you do have to shake it
up to make sure it dissolves. We purchase ours from BBC Biochemical for a very
reasonable price and then test before dumping. We have
We have stains from at least twenty years ago, judging by their
labels--they have no date on them! I always wondered how long neutral red
lasts, but apparently, it lasts a long, long time.
We are not a clincial lab though, we do research.
Emily
By bitching and bitching and bitching, they could
Denise
We use paraplast and paraplast extra and have not experienced any problems. We
just got a new lot of paraplast extra in and so far everything seems ok. I
would check to see if it's a particular lot number that you are using.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory
It depends on the antigen. I know that sounds like a cop out but it is
true. Review the literature covering the antigen you are looking for to
see what has worked for others
Geoff
On 4/11/2013 6:19 AM, Ian R Bernard wrote:
Pretreatments are used to recover bonded antigen sites owing to
Forgot to mention this is on FFPE tissue
From: Elizabeth Cameron
Sent: Thursday, April 11, 2013 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: Anti-human nuclear antibody
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody
MAB1281, and if so, what was
Generally under- fixation in formalin is more of a problem than longer
fixation. The reason is first that less fixation does not preserve the tissue
and antigens as well (more extraction of proteins in the processing steps), and
second, antigen retrieval of any type is more damaging to
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody
MAB1281, and if so, what was your protocol? We are trying to detect human
cells in mice.
Thank you.
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
The
Hi Histonetters!!
I hope everyone is having a great day. I was asked by a friend to forward
this information to you about the Histology Society of Ohio's
Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn
Cleveland- West
For more info and the program schedule please go
Elizabeth,
We are able to detect human cells in mice organs using MAB1273
anti-mitochondria antibody (1:50) from Millipore on FFPE sections after
standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on
Mouse IF detection kit (MaxVision, cat.No MF03),
Anatoli Gleiberman,
I have worked with whole slide scanners and digital image analysis since 1998.
We currently own scanners from 3 different vendors. Also, I have participated
in whole slide scanner marketing research, sitting at a discussion table with a
dozen people from different hospitals.
My experience
Everyone,
I currently use a Nikon Digital Ice 3, Coolscan IV ED to scan 35 mm photos.
Lately, it is often scanning a magenta color or at times either a faded or
blurry image and I know I will need to replace it soon. Can anyone recommend a
reasonably priced, good quality 35 scanner? It
Hello All,
I would like to use a PAP pen to separate sections on my slide, but have been
using an aqueous (non-xylenes based) mounting media. How do you remove the PAP
pen without using xylenes? Is this done?
Thank you,
~Ally
Research Technologist
Comander Lab - Ocular Genomics Institute
I think Scott makes a good point about having a clear need. We use the Vectra
system now, and have previously used Ariol. We only scan IHC slides from
projects in which we want a quantitative analysis.
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box
We just leave the pap pen on. Since it's not covering the tissue, it won't
matter.
Emily
By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.
-Chuck Palahniuk, Haunted
Hello Histonet! I am looking into small countertop fume hoods or maybe a
filter system that I can use to place tissue under that has been in
formalin or decalcifier to diminish the fumes. Do you have any
recommendation on what and where to look for something like this?
Thank you!
- Merissa
Hello everyone I have a question about labeling slides and blocks, I run a POL
GI lab I was just certified by CAP and I wanted to understand if it is
necessary to write GAR13-0123 on both the slides and blocks. We write the
patients last name on both, but writing the GAR and an extra 0 is
We have a formaldehyde test kit. It's a dip stick type test.
Sent from my iPhone
On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote:
Can I ask how you test before dumping?
Thanks
Mark
On Apr 11, 2013 6:21 AM, Cristi Rigazio cls71...@gmail.com wrote:
We neutralize
Hello Histonet! I am actively searching for a night shift Histotech with
experience to work in my Shelton, CT lab. Please feel free to reach out to me
via email for more details or pass this information onto your contacts.
Thank you and I hope everyone is well!
Cyndi Cochran
Sr. Staffing
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