[Histonet] HIER pressure cookers....

2013-04-30 Thread k. leigh adams
Any input as to preferred instruments would be greatly appreciated... Leigh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HIER pressure cookers....

2013-04-30 Thread Connolly, Brett M
Biocare Decloaker here !! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] HIER pressure cookers....

2013-04-30 Thread Blazek, Linda
I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] HIER pressure cookers....

2013-04-30 Thread Jan Shivers
Third that. Jan S On Tue, Apr 30, 2013 at 8:58 AM, Blazek, Linda lbla...@digestivespecialists.com wrote: I second that! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com

RE: [Histonet] 5 gal specimen container search

2013-04-30 Thread Hannen, Valerie
Jackie... We have ordered these from a company by the name of Centurian Medical. The lids are sold separately. Valerie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Wednesday,

RE: [Histonet] HIER pressure cookers....

2013-04-30 Thread Dingersoll
dingers...@aplaboratories.com Original Message Subject: RE: [Histonet] HIER = pressure cookers From: Blazek, Linda [1]lbla...@digestivespecialists.com = Date: Tue, April 30, 2013 9:58 am To: Connolly, Brett M

AW: [Histonet] BIG PROBLEM

2013-04-30 Thread Gudrun Lang
Too much blue is mostly a problem of too less red. Have you checked your Eosin? pH? Rinsing after eosin? My personal issue was sometimes too thin sections. Those looked also rather pale. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Processor question - alcoholic formalin vs. buffered formalin

2013-04-30 Thread Lake, Kim S
I attended the Tri-State Symposium in Dubuque, IA last week (which was fantastic) and sat in on a Boot Camp for Histology workshop. At this workshop I realized that some problems we have been having with sectioning could be due to our processor set up. Before microtomy we have to soak our

RE: [Histonet] Processor question - alcoholic formalin vs. buffered formalin

2013-04-30 Thread Hannen, Valerie
We use 10 % Neutral Buffered Formalin. It is a wonderful fixative and gives us really great fixation. It does sound like you are overly dehydrating your tissues. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida

[Histonet] cassette labelers

2013-04-30 Thread Margiotta-Watz, Michele
Hi All, We are looking to purchase 2 cassette labelers and would like some feedback on which ones you might recommend. Also, is there a difference between Thermo's Printmate and Microwriter? Looks like the Printmate is just a newer version. Your input would be greatly appreciated. Thanks,

[Histonet] HIER Pressure Cookers

2013-04-30 Thread Eichorn, Katy
We use a Cuisinart Pressure Cooker that we purchased at Cell Marque. Basically they buy these pressure cookers and validate them at their facility, I think it was around $400. Works great! Katy Eichorn, HT (ASCP) | Histology Supervisor | Oro Valley Hospital | 1551 E. Tangerine Road | Oro

[Histonet] Monitoring of IHC staining trends

2013-04-30 Thread Troutman, Kenneth A
Hello Histonet, I have a question for the group at large. How are labs monitoring drift in IHC staining over time? Here's the scenario: You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark. Now, you've been looking

[Histonet] Older decal methods

2013-04-30 Thread Mayer,Toysha N
Hi, I was wondering if anyone still uses the ion-exchange decal method and/or the electrolytic decal method? If so, could I get some pictures to put in my power points online? Also, to any of the new HT or HTL's are there any questions on the registry exam about those methods? My latest

[Histonet] RE: Histonet Digest, Vol 113, Issue 30

2013-04-30 Thread Barr,Kaye H
What title do most use for someone who does gross and is not a PA? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To:

[Histonet] RE: Monitoring of IHC staining trends

2013-04-30 Thread Sebree Linda A
Ashley, Every new lot of antibody we get in is run with some of our control tissues and compared to the current/previous lot staining. So we should be able to detect a change. Our policy is that minor changes to protocols are OK but anything major would require revalidation...we try not to

RE: [Histonet] cassette labelers

2013-04-30 Thread WILLIAM DESALVO
I suggest you look at either a heat transfer or laser etch product. The laser jet printers can be a bit of a maintenance issue. Also, you need to choose by the information needed on the cassette. 2D bar coding will take some time to print and requires precision in the printing. This is an

[Histonet] RE: Histonet Digest, Vol 113, Issue 30

2013-04-30 Thread Vanessa Perez
Grossing Tech Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barr,Kaye H Sent: Tuesday, April 30, 2013 12:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest,

RE: [Histonet] RE: Histonet Digest, Vol 113, Issue 30

2013-04-30 Thread Dingersoll
dingers...@aplaboratories.com ---= - Original Message Subject: [Histonet] RE: Histonet Dige= st, Vol 113, Issue 30 From: Barr,Kaye H [1]khb...@mdanderson.org Date: Tue, April 30, = 2013 1:48 pm To: '[2]histonet@lists.utsouthwestern.edu'

[Histonet] RE: Histonet Digest, Vol 113, Issue 31

2013-04-30 Thread Nancy Schmitt
Michele- I agree with Mr. DeSalvo. These vendors will be HAPPY to come in an set up a demo. Some products are better than others, but it needs to fit into your workflow to be successful. We ended up going with General Data and have been very happy with their product. Nancy Schmitt MLT,

Re: [Histonet] Monitoring of IHC staining trends

2013-04-30 Thread Rene J Buesa
In your example you acknowledge that your pathologist is right. In consequence you go back to your protocol and, more importantly, to the lot that cause your pathologist's concern and analyze if it contains the same amount of antibody. By the way, you should have tested the lot to avoid the