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Dear Pat,
here some inputs. If usefull, you have to decide for yourself.
Ad controls: We use for most of our antibodies an universal-control. This
is put together with tiny pieces of normal tonsil, appendix, skin, liver and
pancreas. If you are interested, look at the website of NordiQC.
Some
Pat,
Right off the bat I can see 2 things that could speed things up for you. We
have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't
stay on well, try some Stay On in your water bath; we're experimenting with
that now. Also, we spend way too much time ourselves
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in
formalin for 48 hrs. Since the tissues could not be processed after the 48 hr
fixation they were transferred to 70% ETOH for about a week, then processed and
stained with HE.
The HE staining looks
I forgot to mention that the HE was performed in another lab at our
facility...most likely on an autostainer. We just did the IHC studies and I
have always used 70% ETOH for tissue storage ... never heard of this staining
phenomenon happening before.
Brett
-Original Message-
From:
I am interested in the answer as I use this same technique on my rodent
samples and have not had this issue.
On 7/2/2013 8:07 AM, Connolly, Brett M wro
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in
formalin for 48 hrs. Since the tissues
Hi Brett,
That the HE staining looks faded after storage in 70% seems very unlikely. We
do this with rat and mouse tissues all the time. Did the other lab use a
different Hematoxylin, Eosin, or used a different staining protocol on the
autostainer?
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Do any of you do this? Do any referral labs offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Linda,
we do it here
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org
I would be interested in this information as well.
Cindy
Cindy Pyse CLT, HT(ASCP)
Laboratory Manager
X-Cell Laboratories of WNY
20 Northpointe Parkway Ste 100
Amherst, NY 14228
716-250-9235 Ext. 232
cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Brett,
I agree with the others, the storage in 70% ETOH is likely not the cause of the
washed out appearance of the staining.
* Did the lab that did the HE staining run a daily control? How did that
look? Perhaps the tap water they are using went a little funny? Perhaps the
depar
Re: HE Staining
We do not have a high volume of rat tissues and thus stain by hand. Generally
we have increased the time in hematoxylin and eosin slightly because the tissue
is so dense, even if cut at the same thickness as mouse tissue. We do long term
storage in 70% EtOH and have not had any
Can anyone recommend a good antibody made in rat that works in mouse tissue
that is a nuclear marker and another one that is a membrane marker?
Can anyone recommend a good antibody made in goat that works in mouse and
rat tissue that is a nuclear marker and another one that is a membrane
Yes, I agree with most.
But remember a major part of dewaxing sections is to heat the sections to
remove as much wax as possible.
This allows the xylene or hydrocarbon dewaxing to be as efficient as it can.
Remember to be wary of drying temperature, too high and some antigens (eg S100,
5D3,
You could try 10 minutes treatment with periodic acid prior to HE staining
(Luna's technique I think)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
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