[Histonet] If going to holiday/vacation, or to unsubscribe

2013-07-02 Thread Lee Peggy Wenk
If you are going to vacation/holiday, please unsubscribe from Histonet. We don’t want a zillion messages saying “I am out of the office.” If you no longer want to receive Histonet emails because it isn’t your cup of tea, then please follow these directions on how to unsubscribe: - Go to the

AW: [Histonet] Immunohistochemistry TAT

2013-07-02 Thread Gudrun Lang
Dear Pat, here some inputs. If usefull, you have to decide for yourself. Ad controls: We use for most of our antibodies an universal-control. This is put together with tiny pieces of normal tonsil, appendix, skin, liver and pancreas. If you are interested, look at the website of NordiQC. Some

[Histonet] RE: Immunohistochemistry TAT

2013-07-02 Thread Sebree Linda A
Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some Stay On in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves

[Histonet] HE staining question

2013-07-02 Thread Connolly, Brett M
Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with HE. The HE staining looks

[Histonet] RE: HE staining question - some clarification

2013-07-02 Thread Connolly, Brett M
I forgot to mention that the HE was performed in another lab at our facility...most likely on an autostainer. We just did the IHC studies and I have always used 70% ETOH for tissue storage ... never heard of this staining phenomenon happening before. Brett -Original Message- From:

Re: [Histonet] HE staining question

2013-07-02 Thread Colleen Forster
I am interested in the answer as I use this same technique on my rodent samples and have not had this issue. On 7/2/2013 8:07 AM, Connolly, Brett M wro Histonetters - We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues

[Histonet] RE: HE staining question

2013-07-02 Thread Bea DeBrosse-Serra
Hi Brett, That the HE staining looks faded after storage in 70% seems very unlikely. We do this with rat and mouse tissues all the time. Did the other lab use a different Hematoxylin, Eosin, or used a different staining protocol on the autostainer? Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC

[Histonet] IHC Elastin stain

2013-07-02 Thread Sebree Linda A
Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list

[Histonet] RE: IHC Elastin stain

2013-07-02 Thread Houston, Ronald
Linda, we do it here Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org www.NationwideChildrens.org

RE: [Histonet] DAB Testing

2013-07-02 Thread Cindy Pyse
I would be interested in this information as well. Cindy Cindy Pyse CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories of WNY 20 Northpointe Parkway Ste 100 Amherst, NY 14228 716-250-9235 Ext. 232 cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Re: HE staining question

2013-07-02 Thread Teri Johnson
Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the HE staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar

[Histonet] RE: Histonet Digest, Vol 116, Issue 1

2013-07-02 Thread Jennifer Garrigan
Re: HE Staining We do not have a high volume of rat tissues and thus stain by hand. Generally we have increased the time in hematoxylin and eosin slightly because the tissue is so dense, even if cut at the same thickness as mouse tissue. We do long term storage in 70% EtOH and have not had any

[Histonet] markers in specific species

2013-07-02 Thread pruegg
Can anyone recommend a good antibody made in rat that works in mouse tissue that is a nuclear marker and another one that is a membrane marker? Can anyone recommend a good antibody made in goat that works in mouse and rat tissue that is a nuclear marker and another one that is a membrane

[Histonet] RE: Immunohistochemistry TAT

2013-07-02 Thread Tony Henwood (SCHN)
Yes, I agree with most. But remember a major part of dewaxing sections is to heat the sections to remove as much wax as possible. This allows the xylene or hydrocarbon dewaxing to be as efficient as it can. Remember to be wary of drying temperature, too high and some antigens (eg S100, 5D3,

[Histonet] RE: HE staining question

2013-07-02 Thread Tony Henwood (SCHN)
You could try 10 minutes treatment with periodic acid prior to HE staining (Luna's technique I think) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead