All,
i would appreciate any user experience with the latest version of Hamamatsu's
digital slide scanner (NanoZoomer 2.0-HT C9600-13) that is fitted with the
fluorescence imaging module -- link below. We've had our NanoZoomer (with
fluorescence) for over 7 years and it has been reliable
The wrinkling can happen sometimes when cutting 4%PFA, sucrose cryoprotected
brain samples. Flat sections that wrinkle when picked up on the slide. Using a
soft brush, moisten it with distilled water and swipe it across the area of
pickup on your slide, then pick up your section. It will
I'm trying to get our Co-path computer system set up to print labels instead of
us handwriting on the paperwork and containers...what information do you guys
have on your printed labels...just patient name and accession no? Thanks!
___
Histonet
Dear Histonetters,
I have some mouse bones samples which have been fixed in 70% ethanol
for a few weeks and stored in 80% ethanol for a week; now our
colleague would like to decalcify them in EDTA for wax embedding and
staining. What is the best way to remove the ethanol before transfer
to EDTA?
Dear Histonetters,
Has anyone ever seen large holes/vacuoles in the bone marrow of mouse
long bones after decalcification and processing to wax? We've had this
problem with a study which was fixed in 4% paraformaldehyde and
decalcified at 4 degrees C over a 14 day period according to a
Orla
I know this might sound silly but normal mouse bone marrow contains both fat
and hemoatopoietic marrow, the areas that are fat will appear like round
voids. Is that what you are seeing?
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Hi All, your thoughts on the best toluidine blue method for mast cells?,
many thanks.
Richard Edwards
University of Leicester
U.K.
Whoops! I guess you probably weren’t asking about slide labels. For container
labels, it is barcode, patient name, account number, specimen source, and
container number.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
Richard
We use 0.04% T. Blue in an acetate buffer that is pH'd to 4.0. Stain for about
5 minutes, rinse in water, let air dry and then coverslip in xylene. You will
loose the metachromatic staining if you run the slides through alcohol. I have
an SOP if you would like to see one.
Liz
Try the one on stainsfile
(http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm). It gives
dark blue mast cell granules and is not removed with ethanol dehydration.
Bryan Llewellyn
Edwards, Richard E. wrote:
Hi All, your thoughts on the best toluidine blue method for mast
Hello histonetters,
Who do you use for large brain slides (monkey, pig) to do the studies? Contract
labs are welcome to contact me too.
Thanks,
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
Hello All!
I am having some bizzare staining issues. The eosin seems to be washing out??
The slides look sun bleached according to the Pathologists. The problem is
that it is only happening on occasion. One rack stains fine and the next one
has a problem. It even appears to be
What is the best technique th process scaffolds? we are using adipose
tissue engineering with an alginate/gelating scaffold. Thanks
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I was wondering if anyone bills insurance companies for a 88363 cpt code? This
is for retrieving archived blocks and slides, packaging them and sending them
out to outside facilities for molecular testing (PTEN, KRAS,etc). If you do
bill, what is the charge?
Thanks,
Ron
And I should clarify - that is the technical part. We bill separately from the
pathologists. I don't know what their price is.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA
Hi,
I realize this is a longshot, but the research principal investigator I work
for asked me to inquire. Does anyone have an Aperio ScanScope they would
consider donating or selling at a greatly reduced price?
Thanks,
John
-
John N. McGinley
Cancer Prevention
What about section-thickness? Do you have a tech, who cuts very thin?
I have sometimes this problem, that the staining looks very faint because
the slide is just too thin.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
John
There are plenty labs out there that will scan slides as a fee for service we
do that for CSU already for the microbiology department. There are also some
less expensive scanners out there that scan one to five slides at a time that
would be less expensive than an Aperio Scan Scope
Liz
Hey Histonetters,
Has anyone noted negative staining with the anti-human hepatocyte antibody
Clone OCH1E5 on hepatocytes surrounding the centrilobular vein? We are staining
mouse liver samples and get great staining on about 80-90% of the hepatocytes
in normal liver but then see very low to
Is there a Technical charge (TC) for 88369 or is it only a Professional
charge that the pathologist bills?
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour
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