RE: [Histonet] IHC after EDTA decalcifying

Perhaps I'm missing something here too. Did you decalcify the tonsil for the same length of time as the mouse femur? If you are looking at e.g. CD markers in bone or indeed bone marrow, it is important that you have positive control material that you know works with undecalcified tissue and a

[Histonet] Cryostat decontamination

Just curious how often everyone is taking their cryostats down, I know cap says every week if used daily. Thanks ahead for the info. Everyone have a great weekend Anita Dudley Providence Hospital Mobile Alabama

[Histonet] Cryostat cleaning

ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Cryostat cleaning

Ann, There are many tuberculocidal cleaners. Just search for it. If you are in a hospital the central supply will most likely have some in stock. We use PD-128 by Spartan Chemical http://www.spartanchemical.com/products/product/101604 Here are some references for cryostat cleaning:

RE: [Histonet] Cryostat cleaning

We wipe out the excess tisue( if there is any), wipe down the chamber with abs. Alcohol then spray the chamber with CaviCide, let it sit for @ 3-5 minutes and then wipe the chamber out again. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N.

[Histonet] Dako users

Does anyone out there have Dako IHC and special stains as well as their HE coverslipper? We currently use Ventana and are needing an upgrade soon. Any pros and cons would be greatly appreciated. Thanks! B ___ Histonet mailing list

[Histonet] Re: Histonet Digest, Vol 117, Issue 19

Hi Collen, You had great responses to your question. I do stain mouse femurs for IHC and IF. We fix in NBF overnight and decal for 4-5 days in 0.5M EDTA pH=8 at room temp. After decal we wash with running tap water and re-fix for 4 hrs with NBF. 90% of the antibodies we use on undecal tissues

[Histonet] AUTO: Bruce Palmatier is out of the office (returning 08/19/2013)

I am out of the office until 08/19/2013. I will be out of the office today. If you have a question about an order or need assistance placing an order, or if the matter is urgent, please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service associate will assist you. For quote

[Histonet] EDTA decal

Hi Colleen, I would say it's unusual, but not completely impossible that EDTA has interfered with your IHC. We had that problem with demonstrating B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount staining in X-gal, the blue staining was removed. But if we

[Histonet] post-fixation for Mallory Trichrome

Hello, There seems to be a lot of suggestions for Mallory Trichrome staining involved in Acid fusin, Aniline blue, and orange G. My sample of avian embryos were fixed with Formalin based fixative (4% paraformaldehyde in phosphate-buffered saline and 1% glutaraldehyde) overnight, in case of