I know this question has been asked before but what's the best and safest
Xylene Substitute?
Ruth Yaskovich
N.I.H. N.I.D.C.R.
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I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for
animal tissues?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
We use Propar, mostly for mouse and rat eye samples, it works well in our hands
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com
Ship to address:
Premier
Ruth:
I all reality none is good. Both alkanes or D-Limonene are either as dangerous
or more than xylene, and all perform below the xylene standard.
Any one requires modifications of all procedures, they seldom dewax adequately
and all constitute a processing compromise. Many cannot de recycled.
Donna S. Ing= ersoll, B.S., HTL, CT(ASCP)
Laboratory Manager
A P Laboratories, LLC
2090 Executive Hall Rd S= uite 165
Charleston= , SC 29407
843-300-= 3001 X 202
843-300-= 3003 (fax)
[1]dingers...@aplaboratories.com The contents of this message,
together = with
At another place I've worked for, we used Propar for animal tissues and it
worked well.
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From:
Hello,
I need to order agar to embed rabbit arteries prior to processing. I was told
to purchase Difco #0140-01. There are so many different types. What agar do you
use and from what vendor do you purchase it?
Thank you in advance for your help,
Karie
--
Karie L Durynski A.S.
New Bolton Center
Thank you!
I have added extra step of 5 min of 1% phosphomolybdic before aniline
blue-orangeG, which increased the blue contrast in late stage embryos but not
early stage ones.
I did not realize that Mallory trichrome react differently between embryonic
and adult samples. I just assumed the
Donna:
Please go to the web site I indicated in my previous answer and you find
detailed answer.
After you stain the sections (regardless of the procedure) you dry them in an
oven at 60ºC for 5 mins. and they are completely dry (ANATHEMA!!! to most)
you just place them in your automated
We are having a discussion as to whether the requisition needs to remain
with the specimen at the grossing stage or can it be in a separate stack
on the grossing table, while the actual specimen is left in the on deck
tray. I believe I have seen something that speaks to this through the
CAP
I don't know about CAP guidelines, but I always thought separating the
paperwork was to avoid blood/body fluid and/or formaldehyde contamination
to the poor transcriptionists and clerical people who have to handle the
req after grossing, with no gloves or ventilation! Some places I've worked
have
I am not aware of any CAP regulation that requires the requisition to remain w/
the specimen. The pathologist/PA at gross dissection needs to be able to
positively confirm/verify the specimen container information (two forms of
patient ID and specimen source) w/ the order entry information
There are times when the requisition and the specimen are labeled
differently. Many offices pre-label their specimen containers, take the
specimen from a different source and forget to change the label on the
container. They make the change on the requisition and then you have a
discrepancy.
That seems to happen pretty often. When I accessioned a lot it was at least a
daily occurrence that information would not match between the jar and
requisition, or information was missing or illegible. I have noticed that it is
much easier to reach the actual person who labeled the specimen for
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