Excellent idea!!
By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.
-Chuck Palahniuk, Haunted
On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks amosbro...@gmail.com wrote:
Good morning all!
One of our fellows emailed me a question that she came across while studying
for her boards:
I'm studying for my board exam and came across questions re: paraffin
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on
It's a bad question. Generally the best temperature is probably between 55
and 65, depending on the paraffin. This would fall between the two latter
answers. Hmm What to do?
Phil.
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
The last answer will cover most paraffins used routinely and may cover
microwave also. Temperatures below 58C are often melt points however; they may
not be a complete liquid or have slightly increased viscosity.
Pam Marcum
UAMS
-Original Message-
From:
Hi Histonetters!!
I hope you all had a great weekend!! And I hope your teams NCAA and NFL did
better than mine did!! I wanted to take a minute and tell you about a
position I recently was engaged on that I am very excited about.
RELIA has been engaged to recruit for a histology supervisor for a
I was happy to read your thoughtful evaluation of the problem and, after having
read through it, wonder why I hadn't thought of it before! Perhaps a little
tweaking by the listserv management would make this process much more clear.
The sign-on page of information about Histonet is rather
I'm going to wade in, not as someone who has posted numerous times on how to
unsubscribe, but as someone assessing it from a risk assessment
evaluation.
If there is a lab task that is consistently being done wrong, by many
different people, it is usually NOT the fault of the people. It is
Is anyone running ICC on previously Wright-Giemsa stained smears? What kind
of info can you offer? We didn't have any staining without antigen
retrieval, so we added that step. So with the antigen retrieval, the cells
are in good shape, but now there is too much background without very much
Peggy,
I don't think anyone else could have said it better.
Thank you
Dusko
From: Lee Peggy Wenk lpw...@sbcglobal.net
To: Manfre, Philip philip_man...@merck.com; nmhi...@comcast.net; HISTONET
histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013
On Sunday, Sept. 22, from 8 am - 9:30 am at the NSH Symposium in Providence,
RI, Beth Cox, HTL/SCT(ASCP)QIHC is presenting a workshop on Work and Play
Across the USA - A Guide to Being a Traveling Tech.
http://www.histoconvention.org/
Click on Schedule
Peggy A. Wenk, HTL(ASCP)SLS
Jeffrey S. Silverman HT HTL QIHC (ASCP)
Operations Manager
Anatomic Pathology Specimen Processing,
Laboratory Safety Officer
Lenox Hill Hospital
12 Uris- Anatomic Pathology
100 East 77th Street
New York, New York 10075
office- 212 434 6673
cell-631 375 4505
email: jsilver...@nshs.edu
My techs do the scoring for the FISH testing. We have a Leica Ariol system -
the HER2 FISH are computer assisted, but the Urovysions are manually assessed.
That may change with the new systems we are receiving. My techs are
exceptionally well trained in morphology - they sit with our
Histonetters,
Does anyone out there have any information on traveling histology technician
jobs? What is the compensation? How does insurance work? What qualifications
are needed? I am particularly interested in any personal experiences any one
has had.
Thanks!
CJ Christopher P. Jacobs, HT
Recommended melting point of paraffin is 2-4 degrees above the melting
point of the paraffin. Because we really don't see paraffins that would
have a melting point of 46, the BEST answer would be 58-70. Perhaps not
what we do, but the best answer for the choices provided.
From: Martin,
Hello every body,
I wounded if any of you is working with VIP antibody ( rabbit)?, Any advice for
selecting right supplier will be
appreciated. The best of this antibody used to be presented by Eurodiagnostica,
which unfortunately they are not
presenting it any more for IHC project ( they
Rene,
You still have not answered the question - what does the O stand for?
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
May be the position of the active group on the benzene ring - ortho vs.
meta vs. para ?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham,
Andrea L - (algranth)
Sent: Monday, September 09, 2013
As you are finding out the answers to dye nomenclature you can had these
endings to your list of the O' in Oil red O:
Oil red EGN
Oil red 4B
Ada Feldman
Anatech Ltd.
1020 Harts Lake Road
Battle Creek, MI 49037
Phone: 800.262.8324
Phone: 269.964.6450
Fax: 269.964.8084
I'm currently recruiting for an IHC Manager for a facility in southern San
Francisco. ASCP Certification is required. Feel free to contact me directly
with any questions.
Anna Nolan
Recruiter
Prometheus Healthcare
Direct Line 301-693-8908
Office 301-693-9057
Fax 301-368-2478
I wrote that apothecary names are meaningless = they do not have to have a
specific meaning that, in this case, I do not have the foggiest idea! It does
not have to be anything specific.
Something is for sure: it has nothing to do with orange.
René J.
From:
Does anybody have protocol for Alizarin
red/Alcian blue (also counterstain with something else?) on sections. All I can
find is either stain or double staining on whole mounts.
Thank you in advance,
Rui TAHARA
PhD student
McGill University
Hi all
I was requested where the O stands for. who has any suggestion.
Regards Piet Visser
Histotech Bronovo The Netherlands
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
If that is the case, ORO will stain fat droplets in Frozen Sections. In FFPE
will stain nothing.
René J.
From: Mitchell Jean A jmitch...@uwhealth.org
To: 'Rene J Buesa' rjbu...@yahoo.com; P.E. Visser p...@xs4all.nl;
histonet@lists.utsouthwestern.edu
I'm not sure, but whatever you do... don't store it next to the Sudan Black
B...you'll stink up the lab!!
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
It is for Sudan Red 5B. All these apothecary names are essentially meaningless
René J.
From: Jennifer MacDonald jmacdon...@mtsac.edu
To:
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu;
histonet-boun...@lists.utsouthwestern.edu; P.E.
Please if there is an exact list of duties let us all know.
We have had trainees and we basically taught embedding, processing and
staining, in other words basic Histology. This included special stains as the
persons involved would be taking their registries after one year of training.
There you have it!
René J.
From: Ada Feldman adafeld...@anatechltdusa.com
To: histonet@lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 4:13 PM
Subject: Re: [Histonet] Oil red O
As you are finding out the answers to dye nomenclature you can had these
I believe the answer should be - Oil Red O is a brand name (not uncommon in the
dye indutry) and represents the two dyes found in the compound, Solvent Red 27
and Sudan Red 5B
William DeSalvo, BS HTL(ASCP)
Date: Mon, 9 Sep 2013 13:32:08 -0700
From: rjbu...@yahoo.com
To:
Just like Formalin, a brand name now used interchangeably with formaldehyde.
And your trivia of the day:
Formaldehyde was the first polyatomic organic molecule detected in the
interstellar medium
Courtesy of Wikipedia.
Tim Morken
UCSF Pathology
-Original Message-
From:
Wax-it in Canada should be able to do this. Here is the contact information:
Contact: Aruna Somasiri
202 - 2386 East Mall
Vancouver, B.C., V6T 1Z3
Phone: 604-822-1595
i...@waxitinc.com
www.waxitinc.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi all:
My lab is starting a project involving neuromas. We are in need of resin
embedding of the nerves, but our facility does not have the
capabilities. Is there a company that offers resin embedding/sectioning
services?
___
Histonet mailing
Also APS:
Jason Thorsten (HT, ASCP)Director, Pathology Services
American Preclinical Services, LLC
8945 Evergreen Boulevard
Minneapolis, Minnesota 55433
direct office-(763)951-8034
direct fax-(763)951-8035
mobile-(612)910-3390
jthors...@apsemail.com
www.americanpreclinical.com
-Original
Not sure because for that designation to be used there have to be 2 equal
groups and Oil Red O has 4 benzene rings and in 2 of them there are 4 methyl
groups in para position so I doubt that the O is for ortho.
rené J.
From: Goins, Tresa tgo...@mt.gov
To:
Hi all,
I'm wondering if anyone can share a job description for an unregistered HT
position. I also need to know if unregistered HT's are allowed to cut
frozen sections. I know there is a limit to what they can do, especially
where IHC and special staining are concerned, but I really don't know
I believe the question is: what does the O in Oil Red O stand for?
Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital Clinics
Neuromuscular Laboratory
600 Highland Avenue
Madison, WI 53792-5132
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I believe the question is what is the O signify.
as in OG6 the O is for orange.
From: Rene J Buesa rjbu...@yahoo.com
To: P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Date: 09/09/2013 12:46 PM
Subject:Re: [Histonet] Oil red O
Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that
stain with ORO
René J.
From: P.E. Visser p...@xs4all.nl
To:
Thanks, this is very timely as I'm looking into temp/travel HT.Any suggestions
where to look, which agencies? Thanks, Jeff Lowen
HT/MLT(ASCP)
Date: Mon, 9 Sep 2013 17:52:34 -0500
From: jaylundg...@gmail.com
To: lpw...@sbcglobal.net
Subject: Re: [Histonet] Travel
I've been traveling for the past 16 years! Not everyone who wanders is
lost.
Sincerely,
Jay A. Lundgren,
M.S., HTL (ASCP)
On Mon, Sep 9, 2013 at 2:00 PM, Lee Peggy Wenk
All I have is a histology assistant description I put together. It is mostly
clerical, instrument up keep and other duties. My employer does not hire
uncertified techs- due to CLIA license, grossing, FISH molecular duties (
high complexity). I hope to be a clinical site eventually, and then
I honestly wouldn't recommend it unless you are financially able to
undergo 6 month plus periods of unemployment. In 2008 I didn't work all
year, only maybe the last 3 weeks. The wages have never come back to the
pre- 2008 levels. The largest staffing agency in the country declared
While you're at it, maybe some one can explain what the
-wah- in diddy-wah-diddy means?
On 3:59, Morken, Timothy wrote:
Just like Formalin, a brand name now used interchangeably with formaldehyde.
And your trivia of the day:
Formaldehyde was the first polyatomic organic molecule detected in
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