[Histonet] Low Temp problem

I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomi

[Histonet] RE: cutting thick brain frozen sections for Golgi stain

I routinely cut 50 or even 200um sections. The brain should have some variations of light beige to it. If it is too white, it is too cold. What are you using? I have found that a microtome works much better for thicker slices than a cryostat. Good luck! Laura -Original Message- Fro

[Histonet] RE: cutting thick brain frozen sections for Golgi stain

Hi Andrea, did you try it with a vibratome? If not, I would recommend it. Thanks, Klaus From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu]

[Histonet] cutting thick brain frozen sections for Golgi stain

I need to cut 80 micron sections of frozen brain. Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration? Andrea Grantham, HT (ASCP) Senior Research

RE: [Histonet] HTL / CG

Joelle, I think you will have a very hard time finding someone with both, especially dual ASCP certification. I've met or hear of anyone with dual certification, or even working in both. It may be the best you will find is someone who has certification in one and some practical experience in the

RE: [Histonet] HTL / CG

Thank you for the information and insight. You are indeed multi-talented. Sounds like whatever direction you go, you will be successful. I am not expecting to find such a combination as HTL/CG, with extensive experience easily. I just wanted to have some numbers, opinions, and information to r

Re: [Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

I second the statmark pens from stat-lab. They are very solvent resistant, don't dry out that easy. They are however a little on the pricey side, at about $5.00 a pen. Patrick Laurie On Wed, Oct 23, 2013 at 1:58 PM, Walter Benton wrote: > Are you using the red label "Industrial Sharpie" alrea

RE: [Histonet] CLIA

Thanks Kim, I agree seems more clinical ... -Original Message- From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] Sent: Wednesday, October 23, 2013 1:22 PM To: Hale, Meredith Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA I can only speak from a path lab point

Re: [Histonet] CLIA

I can only speak from a path lab point of view and from reading the IQCP guidelines it doesn't allow you to use these new measures for path. Path is exempt. and in path I've always used controls for each test. My understanding is in some specialties they are going to let them make individualized

[Histonet] RE: ASR antibodies verses IVD antibodies

Jim, Since companies are not allowed, by law, to tell you any method information about ASR's, it is the responsibility of the lab to do a comprehensive validation according to CLIA regulations. Treat it as if it were an antibody some researcher gave you and you need to figure out EVERYTHING to

[Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

Are you using the red label "Industrial Sharpie" already, if not give them a try? I would also suggest using a slide marking pen since they are resistant to many solvents used in the lab like alcohol and xylene. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 L

[Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

We have had good luck with the Statmark Pen from StatLab - Item # SMP-BK. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the p

[Histonet] Can someone recommend a good labeling pen for cryotubes.

Hi everyone, Can someone recommend a good pen to use to label cryo tubes.The VWR pens we have been using are apparently discontinued. I would like something that writes as fine as an ultrafine sharpie, but is less likely to smear and is more long lasting, and solvent tolerant. Thanks Patr

[Histonet] ASR antibodies verses IVD antibodies

In older CAP regulations ASR's had to be handled separately than IVD antibodies including a disclaimer acquired from the FDA. I don't find that in the current CAP checklist. Has this been eliminated and if so how are people handling validation of ASR antibodies? Are they using the validati

[Histonet] HTL / CG

Hello fellow histology netters I have been asked by my employer to search for and retain an individual who possesses an HTL (ASCP) certification, but who also has a CG (ASCP) certification ( or at least have solid experience in the arena of FISH & cytogenetics). I have not personally come acro

[Histonet] Amacr antibody from DAKO

I am checking into ordering the prediluted amacr antibody from DAKO since it is an IVD. Could anyone share the protocol they are using on the Benchmark Ultra with this antibody and the standard I-view detection kit? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervis

Re: [Histonet] slide drying oven

Hi Laurie, Biocare has one that I have used, they call it the desert chamber pro, one of the few that is setup and designed expressly for drying slides. It works very well, and has different heating options and a timer. However, all you may need is a basic mechanical convection oven, Boekel has

[Histonet] slide drying oven

Can anyone recommend a small, forced air slide drying oven? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwe

[Histonet] Shedding biopsies

Hi Diana, RE: "In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they

[Histonet] New Technical Sales Opportunity - IHC Antibodies

Good morning, We have had a world leading cancer diagnostics client open a new opportunity in the Southwest. Our client is searching for an Antibody Sales Specialist ideally based in TX, OK, AR, LA, or AZ. We are looking for histology professionals who have a strong background in IHC and antib