Hello!
I'm trying to find a new microscope camera for our histology
histopathology teaching lab.
I would like to achieve full HD live image with good color reproduction.
Anyone have experience on the Leica MC120/MC170 HD cameras? They are
quite nice otherwise, but based on a quick demo, I'm
I know the potential for damage to your health is huge in histology, but are
there any studies out there that indicate histotechs are less healthy than the
average person?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
If not interested in webinars designed for supervisors and
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Hi All,
I'm currently recruiting for a histotechnologist in NYC. Must have NY State
Clinical Laboratory Technologist License and a Bachelor's degree for a 2nd
shift opportunity. Please call/email with any questions.
Anna Nolan -
Recruiter
Prometheus Healthcare
Direct Line 301-693-8908
I agree!
If everyone adheres to safe practices we will make it into our golden years.
Let's bring back our NSH health studies~ Vivian McClure would love for us to
continue the studies.
Mike Ayers and Shirley Powell have been my mentors through the years (36 plus
years for me)
Thank you
Couple of studies that I know of.
One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH
Symposiums, and did different tests on people who volunteered to
participate. Published findings in the late 1980's that said that histotechs
had lower pulmonary function than average
I remember participating in the health study in the 80's. Xylene and
Formaldehyde levels are monitored in all labs. If our hospitals/research
centers would allow us to forward that information on to someone who could
compile data it would be a starting place for a health study.
I strongly
I remember the study and some fairly important people in NSH at the time
thinking it was a little overblown. It was not followed up on at the time just
a report in JOH and done. I have been in Histology many years (about 50) and
like Hazel have seen many of the ones even older than us die
The article doesn't even mention the repetitive motion injuries.
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I had the same experience, the clinical lab techs asked the supervisor to keep
our door closed so they would not have to smell the fumes, just lock us up in
it. I was considering calling EPA in to check it out. Thank goodness those
days are gone. Use those fume hoods and all the other ppe
My current lab the air exchange rate is 58/hr.
The good news: you do not smell fumes of any kind.
The bad news: we are negative pressure and the unvented bathrooms are just
outside our lab.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Oh joy!!!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's
Hospital and is intended for the
For tissue, what step most contributes to subsequent swelling of tissue when
the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin
infiltration all contribute, but at what percentage?
Here is the current schedule, which apparently was meant to mimic a microwave
schedule
We are planning our move to using on-slide controls for IHC and I'm wondering
how other labs handle the workflow and logistics of matching controls to stain
orders.
We plan to use a TMA for 80% of our orders. So far we have one TMA that covers
most Ab's but the number will probably will be
We do strictly GI biopsies, but if we have a stat case, we have a processing
schedule as follows: five minutes in each station of one 70%, two 95%, two 100%
and three xylenes, then 10 minutes in each of three paraffins. We have the
ASP300 and this has been very successful. I have heard from
I second Toni's suggestion. Definitely sounds like the paraffin is still
present on the lower sections of the slide.
On Wed, Dec 4, 2013 at 10:17 AM, Rathborne, Toni
trathbo...@somerset-healthcare.com wrote:
Could it be the heating/deparaffinization process? If the upper sections
are
Hi Cherie, Since the top section is consistently better, then the possibilities
lie in what happens after they are on the slide. As Toni recommends slides that
are heated or deparaffinized vertically may have left over paraffin on the
lower regions.Maybe you could also try depar with them up on
This is what we've done when we recently moved to control tissue on every slide
for IHC as well as Special Stains.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
Tim
Of course not every method works everywherehere is what I do for myself.
I use a mix of MTB blocks with some single tissue when scarcity of tissue makes
this sensible. The MTB blocks are based on the IHC panels Make own MTB blocks
, feel too expensive to buy for me right now, but
Thank you for all the suggestions. Nothing more frustrating when you can't
point your finger to the exact problem.
Thanks again,
Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical
Hi Guys-
Does the routine Helicobacter pylori IHC staining generally stain the whole
spectrum of helicos?
We had a clinician ask specifically about the helmani and we're waiting for a
response from the vendor but now I'm curious, too!
Thanks in advance and Wednesday--
Cheryl
Cheryl Kerry,
For a long time I have had our IHC techs print out the run logs from each IHC
run on the Benchmark Ultras. The techs then check the slides to make sure the
positive and negative controls have worked properly before the slides are sent
to the individual pathologists. The pathologists are
Hi,
First I would like to say thanks a lot to you and also Carl who gave me some
helpful tips. Then I would like to ask if you have any practical tips when I
want to pick up the skin from perfused animal. I need those special tricks
which are not normally mentioned in the articles!, I am going
The polyclonal antibody from Dako labels H. helmennia in addition to H. pylori.
Several of the commercially-available mAbs to H. pylori that I have evaluated
do not label H. helmennia.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection
We also have Ultras Jim. We don't print out the run logs. IHC personnel
review the controls before they go to a pathologist in order to catch any
problems. The pathologists are supposed to review the controls associated with
each case they sign out. Our pathology report has a statement
We had same problem at my old job. I don't think they have come up with a
separate IHc so our doctor would order an alcian yellow with ihc on children
under age of 12 I think but we gave this up cause it's so rare. If you look up
on Google under h. Heilmannii, I believe its spelled, it has a
Hello everyone,
Got your Christmas shopping done? (Didn't think so...)
Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is
comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some
at VWR and FISHER (no shipping charges for our lab) that
Oh Poop I mean Damm
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology
Hi Tim,
Try fixing for 45 min at 50oC. I think it possible that the tissue is not
adequately fixed.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine,
Unfortunately some controls cannot be confidently used in a TMA eg CD15 - where
often Hodgkin's cells are sparse in the block, or CMV or adenovirus - again
same reason - sparse positive cells. We sometimes use composite blocks, eg skin
and lymph node for S100 and CD1a - (dendritic cells) or (in
If they are perfused, you don't need to worry about stopping them from curling
( they will be fixed/rigid).
Get as large a piece as possible: preferably two pieces.
(The second piece can then be further fixed for 2hrs before placing into 30%
sucrose until the specimen sinks, then snap-freezing.
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