Incredible! Lee Luna (who wrote the book on histology) always said rehydrate!
rehydrate, rehydrate Where are these so called supervisors coming from???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Ha!
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: susan.wal...@hcahealthcare.com
To: b427...@aol.com; dea.les...@gmail.com; histonet@lists.utsouthwestern.edu
Date: Mon, 6 Jan 2014 02:28:55 -0600
Subject: RE: [Histonet] Soaking artifact
CC:
Incredible! Lee Luna (who wrote the book on
I have been sectioning 28 years and, like everyone, completely disagree, at
least with respect to animal tissue. You can oversoak some tissues like
brain and liver, but you can also cut through the over soaked region just by
cranking the wheel a little under the puffy stuff is gone. Also, the
The only soaking artifacts that I can think of would be caused by:
- soaking too long in water (minutes instead of a few seconds)
- soaking under-processed tissue in water
In both cases, the tissue is supposed to be protected by the wax, and if
it is not (under-processed), or if the faced block
With respect, this may work in a non-industrial setting, but in Pharma, with
high through-put, it is generally not possible to have individualized
processing programs for a lot of different tissues. You have to find one or
two that work for the majority and compensate with some soaking.
One slight amendment - this applies to human tissue.
Animal tissue has far less bound and unbound water to start with, so no
matter how it's processed, it always ends up dry. Therefore, longer
soaking in water is needed.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Lee
Thanks Peggy and Amen! When you have people taking human tissue and facing 25
or 30 blocks at a time and then leaving them face down on ice with water it
causes the issues you stated. Soaking is something that was far more common in
the past as the tissue fixed on processors with less reagent
Does anyone out there use the Amyloid Red stain from Anatech in place of the
Congo Red dye? Due to the pricing, would like some critiques before I order
it!!!
Thanks,
Dorothy Webb
This e-mail and any files transmitted with it are confidential and are
I agree Peggy. Just one question. What is a small histology lab to do when they
only have one processor and cannot run separate cycles and do not have staffing
to run short cycles throughout the day?
Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2
years with Clia, like you do CAP specifically Temperature and
maintenance charts. Thanks Anne
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In most labs, the processor runs all night long. Someone comes in very early
in the morning, empties the processor, starts the purge cycle, and then
starts embedding a lot of blocks.
The tissue processor then sits, doing nothing, from after the purge in the
early morning, until sometime in
Hi Anne,
CLIA Sec. 493.1105 list the retention requirements for records, slides, blocks
and tissue:
(3) Analytic systems records. Retain quality control and patient test records
(including instrument printouts, if applicable) and all analytic systems
activities specified in Sec. Sec. 493.1252
The following was taken from the NCCI manual, Chapter 10, effective 1/1/14:
9. The unit of service for in situ hybridization reported as CPT codes 88365,
88367, or 88368 is each probe staining procedure per specimen. If a single
probe staining procedure for one or more probes is performed on
My understanding was that this is just for Medicare patients...
On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill jjoh...@cpallab.com wrote:
The following was taken from the NCCI manual, Chapter 10, effective 1/1/14:
9. The unit of service for in situ hybridization reported as CPT codes
88365,
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