And what is the buffer of choice, PBS, TBS, with or without
protein/detergents or whatever, thanks.
Richard Edwards
We are using Lysol I.C. for disinfecting the ventana benchmark. I thought I
purchased it from Cardinal but I'm not sure now where we got it. Are others
using this for their machine and where are you purchasing it from? Thanks so
much.
Anita Dudley
Providence Hosp.
Mobile, Al. 36695
Lab Safety Supply sells it as well.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com
Voted a Best Place to Work by
Baltimore and
Good Morning!
Where are you currently obtaining p16 from?
Thanks a bunch!
Nancy
Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Please visit our website: http://www.uclaccess.com/
NOTICE: This email may contain legally privileged information. The information
That's what we use Anita and we purchase it from Cardinal as well.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I believe Ventana owns the rights to that antibody now. It was previously owned
by MTM Labs.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
To calibrate a pH meter, we use purchased pH buffers at pH 4.0, 7.0, and
10.0.
To perform the IHC method, we use TBS (pH 7.6) with Tween 20 as our rinse
buffer.
Jan Shivers
On Thu, Jan 23, 2014 at 2:52 AM, Edwards, Richard r...@leicester.ac.ukwrote:
And what is the buffer of choice, PBS,
Hi all-
Our histopathology lab is looking to
set up an IHC component to service the request of our clients. All work is on
animal tissue. We will probably run about 1,000 IHC slides the first year and
hope to increase the workload each year. Some stains we would run would
include CD4, CD8,
Doing ihc on animal tissue is very tricky, if for no other reason than you are
trying to standardize an antibody across multiple species. You will need to use
every trick in the book and invent some of your own to get reproducible
results.
I would steer clear of any platforms that do not let
I totally agree with William. I do animal IHC and I am, using the
Biocare Nemesis, which be the same as the Dako platform. Completely open
so you can do what you need to at a cost you can afford
Colleen Forster HT(ASCP)QIHC
u of MN
On 1/23/2014 11:44 AM, Will Chappell wrote:
Doing ihc on
Diagnostic Biosystems has the same platform with tech support that is
outstanding!
Michael O. Thompson
Director of Sales
Diagnostic BioSystems
Phone: 1-888-896-3350
Mobile: 412-860-1288
Office Fax: 412-727-6080
IHC Made Affordable
www.dbiosys.com
Colleen Forster cfors...@umn.edu wrote:
I
Erin,
We run the Ventana Discovery XT at our lab and average 15,000 antibodies
stained a year using 5 stainers on animal tissue. If you are working on volume
I would also look at the Leica Bond system. Leica and Ventana both have
released or are releasing more flexible systems as far as
Keep in mind that not all pH electrodes are Tris-sensitive; check the specs on
the electrode you plan to buy if you use Tris buffers.
...
Tamara Howard
Dept. of Cell Biology Physiology
University of New Mexico
Albuquerque, NM
Hello Helpful Histonetters,
I have some samples that were fixed in PFA, placed in acrylamide gel and
then treated with iodine for microCT at another facility. The investigator
now wants to follow up and get HE's on these samples. I know how to deal
with the iodine, but my question is this:
I work in animal tissue only. I also agree that an open autostainer system
works best, since you may have to run multiple dilutions and incubation
times for the same antibody because of the different species' reactivities.
You may also need multiple types of reagents to cover detection in
So Mike,
It appears you are a sales director for the company, which makes your opinion
null and void. Sorry.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Thompson
Sent: Thursday, January 23, 2014
I've used many different platforms and have liked most. Currently we
are using BioCare. We LOVE our BioCare Intellipath FLX for our IHC
platform. It is very cost effective, completely open, and has very user
friendly software. BioCare has been a super company to work with.
Fabulous customer
Hi all,
I am trying to look at eosinophils and neutrophils in FFPE muscle sections.
Does anyone have experience with the Wright stain or a Wright staining kit on
these types of sections? Or possibly a suggestion for a different protocol to
visualize these cells?
Thanks!
Becky
Sorry everyone, how long due most labs retain unstained glass slides? I have
the CAP requirement but due they have requirements for unstained glass slides.
Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA 23235
W: 804-527-1316 | F: 804-270-0917
I second this! Since I've not worked with animal tissue I didn't know quite
how to respond to the question but in regards to flexibility, ease of use and
unquestionably remarkable support I would not hesitate to check into the
Intellipath FLX.
Linda Blazek HT (ASCP)
Manager/Supervisor
GI
I'm working with a lab that stained cat gonads with Warthin Starry.
We're looking for Bartonella bacteria. In the first batch that was
stained, some nucleii filled with the silver stain, something I've see
in published images. In the second batch it appears that nucleolus
filled with silver.
Becky:
You can use Giemsa for tissue sections. under separate cover I am sending my
article on the subject.
René J.
From: Berger, Rebecca berge...@email.chop.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Thursday, January 23,
We use the Leica platforms and just got a Leica RX which is for animal research
and ISH, we love the openness of it.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
rueggihcconsultin...@outlook.com
Hi Jerry,
Are you sure they are not AgNOR's:
Lindner, L. E. (1993). Improvements in the silver-staining technique for
nucleolar organizer regions (AgNOR). Journal of Histochemistry Cytochemistry,
41(3), 439-445.
Thiebaut, F., Rigaut, J. P., Reith, A. (1984). Improvement in the Specificity
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