I am ok with them putting the label on the front as long as it does not obscure
our label. Especially since our label contains bar codes we use for tracking
and storage.
__
Lynn M. O'Donnell, CT (ASCP), MHA
Technical Specialist, Cytology
Danbury Hospital
203-739-6704
We do not cover the referring institution label. Will place our label on
front if there is clear area; if not, our label is placed on the back of the
slide, at the frosted end.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
We are seeking a seasoned Histotechnologist for an Orlando, FL based lab.
This is a Lead position on the 3rd shift (overnight) and is a full
time/permanent opportunity. Send your resume to
resu...@alliedsearchpartners.com to be considered.
To view a complete list of Allied Search Partners current
HI,
What would I use for a control for the Okajima stain (hemoglobin).
This is my first time doing this stain so any advice would help as well.
Thanks!
Betsy
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (f
So...I have never done this before. I looked it up in the good ol' bible and
found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure
because he thought it would be more gentle. So...the slides have been sitting
in the 10% solution for 24 hours now. While it is definite
it doesn't bother me to have two labels/tracking numbers. I retain theirs so
that they keep the liability of identification with the patient blocks and
specimen source since I do not have these items to be able to cross check with
the slides. The in- house ID is really just for in house processi
Experts!
Is there any reason you would not consider formalin fixation followed by
cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter)
staining of mouse muscle?
I ask as many folks seem to have difficulty in the flash freezing aspect
of tissue collection and end up with lot
Hi Sarah,
I've bleached out melanin using a 5% Potassium Permangenate solution. It's
very quick - about 15 to 30 minutes. Wash thoroughly in running tap water.
I've done IHC after bleaching with no damage to the tissue.
Hope this helps.
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnos
I used successfully Oil Red both on flash frozen and formalin-fixed sucrose
cryoprotected samples.
Don't see any reason why not use formalin fixation.
Anatoli Gleiberman, Ph.D.
Director of Histopathology
Buffalo Biolabs LLC
73 High Street
Buffalo, NY 14203
Phone: 716-849-6810x354
e-mail: agleib
David
That does work, we have done it here on mouse muscle and liver, but there are
some problems to fixed and then frozen tissue. The tissue on occasion does
not stay on the slide as well once it sectioned, even with plus slides.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Labor
Hello Everyone -
HistoTALK (www.HistoTALK.com) was invited to the Georgia Society for
Histotechnology's very 1st HISTOPALOOZA. We will be there at Calloway Gardens
in Pine Mt., GA from April 25 - 27 (HISTOPALOOZA 2014 dates) interviewing some
of our community's most sought after speakers.
Fo
We pretty much only used formalin-fixed, frozen tissue for ORO. Just don't
process them - the alcohol will dissolve the lipid out.
Phil.
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486
215-652-9750
215-993-0383 (fa
Amber McKenzie (where?) asks: >>Would it be rude to ask other hospitals
that review my slides to NOT WRITE their accession number on the front of
my slides, but instead put their accession number on the back of my slides?
Sometimes, I get slides back that have stickers on the front under my
accessi
Hello wise ones! I am having an issue with yeast floating off my lab, agar
grown fungus controls. Micro made us a beautiful control 5 months ago. Now all
of a sudden we are seeing a significant amount of yeast floating off the
control and attaching to the outer edges of the patient tissue. I t
Jim, We used to do multiple bone labels at time points in large animals. I did
not do the calculations. A veterinarian is the best resource. Also, check
publications by Markel.Bouvy.Manley.Zdeblick - all independent researchers that
used fluorochome labels and identification using PMMA preparati
Hello to everyone!!!
Has anyone used this antibody ?
Novocastra Mouse Monoclonal Antibody Inmuneglobulin D
This antibody is used on FFPE tissue.
Could I please get a copy of your protocols?
I need to know retrieval epitope time and method.
Thank you so much
Aldana Vistarop
___
Hi All , I was wondering if anyone had any advise on getting clots and cores to
stay put? We are using plus slides and giving the slides extra oven time and we
are still having issues. So if anyone has some histomagic up their sleeve
regarding this issue it would be appreciated.
Ginny Miller HT
Amber,
My lab buys half size colored slide labels and we place them on the
bottom of the slide, they are just a little bit bigger than the size of the +'s
on plus slides. We have a printer for consult slide labels which when they are
received we print these half labels. They do not obscu
Cheryl,
If you are having trouble with the yeast floating down the slide and onto your
patient tissue, you can always run the control and the patient tissue on
separate slides. Running separate controls is always a good idea when searching
for microorganisms (GMS, AFB, or PAS-F stains). Also, I
Hello everyone,
I am doing a product survey of sorts and was just wondering which cassette
writer, slide writer and embedding center you were using. I don’t need a huge
product review but any details worth noting would be good. I am equipment
hunting for a lab that will be doing 30-60 clinical
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