Lester,
Probably not in most cases but it depends upon what the antibody is, whether
as a an antihuman ab it also cross reacts to the species you are interested
in and what detection reagents you are using.
For instance if you have determined a rabbit anti human antibody also cross
reacts in mouse
Hello to all in histo-land. Who takes care of releasing the body to the
funeral home and who does the cleaning of the seals and the molds on the
drawers for the body storage? Is lab some how involved in this process?
Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health
Lester
I would not, first of all not all antibodies that work in human will cross
react with a particular species of animal. Another thing to consider is that
most human based detection systems consist of dual link reagents, meaning that
they work on both mouse and rabbit primaries these detec
Dr. Raff,
You will need to do trial runs to first determine if the antibody can
detect the specific epitope in animal tissues (epitopes aren't always
conserved between species), and then do your own optimization to determine
the best dilution and tissue pretreatment for that particular species.
T
Hello Histoland!
A colleague of mine is trying to section unfixed whole rat eyes in OCT. She is
getting a lot of tearing at the top of her section. Lens and optic nerve on
the horizontal plane. Any suggestions, recommendations would be great!
Jackie
__
Hi:
Can IHC validated for humans be performed on animal tissues without any
protocol changes?
Thanks,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
The above checklist item, ANP.22760, states "The performance of new lots of
antibody and detection system reagents is compared with old lots before or
concurrently with being placed into service." Neither the question, the note
below it, nor the evidence of compliance states that a pathologist
only 1/2 way through reading the article, but Terri makes a good point about
the proficiency testing.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Wed, 26 Mar 2014 12:48:46 -0400
> From: tbr...@holyredeemer.com
> To: richard.car...@hhchealth.org; histonet@lists.utsouthwestern.edu
> CC:
> S
Fortunately, they say nothing at all because if that were the case, they
would no longer be able to peddle their Proficiency Programs for IHC,
since those too, are fixed and processed elsewhere.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdo
I have not read the entire document yet. What do they say about using tissues
that have been fixed and processed elsewhere?
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
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