Re: [Histonet] Microtome

2014-06-04 Thread Ronda Mire
Agree, Leica is the best hands down. On Jun 3, 2014, at 5:37 PM, nmhi...@comcast.net wrote: If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd tell them, no, thanks!. I am not inclined to endorse any other brand and I cannot imagine changing my mind about that.

RE: [Histonet] Should I leave histology world

2014-06-04 Thread joelle weaver
Yes thanks for the perspective. I have a bias towards my own experience, and this seems to be good advice. I work in a molecular based lab now and they are very unaware of what it typically is like in a clinical histopathology lab. Good to point other environments are out there that are

[Histonet] fluorescent scope and slide scanner

2014-06-04 Thread Santiago, Albert
Hello my colleagues in histoland, We are in the market for a Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020

[Histonet] Practice tissue?

2014-06-04 Thread Nutter, James E
Go to the local pet store and get a feeder rat (can buy frozen ones). Gross it and process. Great for practice tissues, can get the full range from brain to kidney. Good luck! James E. Nutter Jr. BS, HT QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.|

Re: [Histonet] Should I leave histology world

2014-06-04 Thread Rene J Buesa
You describe a common situation in histology where the pressure to finish the work on time (the TAT) reigns but that does not necessarily mean that quality has to be sacrificed, although sometimes it does. On the other hand, not all histotechs are created equal and some have abilities others

[Histonet] RE: Histonet Digest, Vol 127, Issue 3

2014-06-04 Thread Nancy Schmitt
Hi Denise- I would HIGHLY recommend the online program at IUPUI - we have had 4 people go through this program including myself. When finished you are ready to sit for the test! It is really well done and they are so helpful along the way. Contact info: Debra M. Wood, Director,

[Histonet] HTL program

2014-06-04 Thread Bilger, Andrea
I have several techs who used Indiana University's on line course and they are all great techs! Have not been impressed with techs from Harford Community's program. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040

Re: [Histonet] fluorescent scope and slide scanner

2014-06-04 Thread Rene J Buesa
Contact a Leica Microsystems representative. René J. On Wednesday, June 4, 2014 8:42 AM, Santiago, Albert albert.santi...@uphs.upenn.edu wrote: Hello my colleagues in histoland, We are in the market for a  Slide Scanner and a Fluorescent Microscope, if anyone has any information on any

[Histonet] Clearium?

2014-06-04 Thread Abbott, Tanya
This is a bit more of a Cytology question, but I thought I would survey my Histo friends! We are starting to have bubbles with our PAP slides, and it actually seems to occur after drying, initially after coverslipping (by hand) there appears to be no bubbles. We use Clearium, so we can go

[Histonet] Pathologists?

2014-06-04 Thread Gudrun Lang
Dear histonetters! Maybe you can help me. We are looking for pathologists for our clinical histolab in Linz, Austria. Anyone (German speaking), who is interested in is welcome to inform him- or herself on this website. http://www.linz.at/akh/10374.asp We have a well equipted, modern

RE: [Histonet] Should I leave histology world

2014-06-04 Thread Morken, Timothy
Rene is right that everyone needs to find what they are good at. We had a guy who was so-so in cutting speed and always getting flack for not doing enough. And had such a hard time coordinating specials and immunos that he just slowed things down. Then we started doing Mega blocks of whole

Re: [Histonet] Should I leave histology world

2014-06-04 Thread Emily Brown
As someone who has been in research (basically being a histologist), I can say that there are NO jobs out there for you. The market is saturated with PhDs. Do not leave your job for a research position unless they can guarantee your salary for years. This will be very unlikely, as getting a

[Histonet] RE: fluorescent scope and slide scanner

2014-06-04 Thread Elizabeth Chlipala
Albert It all depends on your through put and how many slides you need to scan daily. There are so many other things you need to consider prior to purchasing a scanner. If you go to the DPA website you can get a lot on information on digital pathology www.digitalpathologyassociation.org The

RE: [Histonet] HER2 by IHC- Fixation

2014-06-04 Thread Weems, Joyce K.
That is true. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the

[Histonet] HER2 by IHC- Fixation

2014-06-04 Thread Adesupo, Adesuyi (Banjo)
Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman

[Histonet] leaving histology question research is still an option

2014-06-04 Thread koellingr
Alpha Histotech,   wanted to be sure that I did NOT tell you to drop everything in life to look to research exclusively.  So I cut and pasted this from my original message Research histology should not be overlooked I stand by that statement.   I agree with Emily that funding in research is

[Histonet] Acid Cleaned glassware

2014-06-04 Thread Abbott, Tanya
We just acquired a brand new laboratory microwave to replace ours from the 1980s,..so we are optimizing our GMS stain. Currently we would let the glassware sit in Chromic acid for 1 hr to chemically clean the glassware before staining. Now we are using the CAP required polypropylene

Re: [Histonet] Acid Cleaned glassware

2014-06-04 Thread Rene J Buesa
Venting the MW oven is just a safety issue and has nothing to do with the cleaning of the glassware and the quality of the staining procedure. René J.  On Wednesday, June 4, 2014 1:01 PM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: We just acquired a brand new laboratory

[Histonet] RE: leaving histology---research still an option

2014-06-04 Thread Teri Johnson
Thank you Ray for your perspective. I wholeheartedly agree with you. A few (maybe more than a few) years back I wrote an article for NSH on transitioning from Clinical to Research. You'll find it here: http://www.nsh.org/content/transitioning-clinical-research In most cases speed is not the

[Histonet] RE:Change in Her2 Neu Fixation

2014-06-04 Thread Terri Braud
As of the 4/2014 revision, CAP extended the fixation time to 72 hours. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 10. HER2 by IHC- Fixation (Adesupo,

[Histonet] slide drying/curing before filing

2014-06-04 Thread Lake, Kim S
According to the Histonet archives, this hasn't been discussed for almost a decade. How are labs drying/curing their slides before they are filed? We are a small oral pathology laboratory and we hand-coverslip using Richard Allen mounting medium and glass coverslips. After cases are signed

Re: [Histonet] Microtome

2014-06-04 Thread Tony Auge
Of the three microtomes that you listed I would highly recommend the Thermo Shandon Finesse. It's of superior quality than the Microms I have used. Cutting is excellent and the fly wheel action is light yet fluid. You do have to oil it manually once a month but it's easy and you can clean it out

[Histonet] IHC patient reports

2014-06-04 Thread aobrien88
How long is everyone keeping the IHC reports that are printed off after the run is completed? ANP.22660 states that Batch control records must be retained to 2 years. Is that also the patient? Thanks ___ Histonet mailing list

[Histonet] CAP Question CYP.04300 Daily QC

2014-06-04 Thread Piche, Jessica
Hi Everyone, I am asking a question for our Cytology department about CYP.04300 on the CAP checklist, in regards to daily QC. It states the technical quality of the preparations should be checked daily and includes checking all stains for predicted staining characteristics each day of use.

[Histonet] RE: IHC patient reports

2014-06-04 Thread Morken, Timothy
The patient results are in the final report. The 2 year requirement is for QC documents only. Tim Morken Supervisor,Hisotlogy, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From:

[Histonet] Great Job Opprotunity

2014-06-04 Thread Jackie Pitts
Hello all! I am posting a great opportunity for a confident and experienced histotech. It is located in Baxter, MN. You would be working for Dr. Lundstrom at Dermatology Professionals. The job is to for the most part run the histology lab. Right now this is my job and I am leaving only because

[Histonet] microglia antibodies

2014-06-04 Thread Kiousis, Sam
I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results. Does anyone have any suggestions or

RE: [Histonet] Practice tissue?

2014-06-04 Thread Anne Murvosh
Contact your local labs and Hospitals, you could just use some already embedded and cut tissue that is about to be disposed of as long as there is no patient info on the blocks it shouldn't be a problem. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: microglia antibodies

2014-06-04 Thread Elizabeth Chlipala
What species are you looking to stain? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day

[Histonet] How to ask a question on Histonet?

2014-06-04 Thread Epp, Carol SktnHR
I would like to ask a question:Headline-MICROSCOPIC CHECKS- What slides do you microscopically check before sending out to the pathologist? carol@saskatoonhealthregion.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: Acid Clean Glassware

2014-06-04 Thread Terri Braud
Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your

[Histonet] Alpha Histotech - leaving the world of histology

2014-06-04 Thread Victoria Baker
Hi Alpha, I've been ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Validating slides

2014-06-04 Thread Sharon Scalise
I am looking for comments about validating slides in histology/immuno. We have always validated our charged slides if we went to a new brand/vendor in order to ensure that the tissue sections will adhere properly during immuno procedures and certain special stains. Once we found them to be

[Histonet] RE: Acid Clean Glassware

2014-06-04 Thread Tony Henwood (SCHN)
No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience

[Histonet] GMS better than PAS for Fungi

2014-06-04 Thread Tony Henwood (SCHN)
Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that