[Histonet] Cytokeratin AE1/AE3

Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically in-between the cells. We never had this

[Histonet] out of office reply

I will be out of the office on Thursday 7/10. If you need assistance, please contact Sue Bauer at sba...@cbgbiotech.com or x203. Regards, Cary Ward Technical Support Respresentative 800-941-9484, ext 220 cw...@cbgbiotech.com ___ Histonet mailing

[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA

Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to

[Histonet] Re: Ventana Ultraview users

Beth, We are doing exactly that same thing right now. This is changing a complete detection system, so we need a full validation (10 pos + 10 negs for all non-prognostic markers). To avoid running 20 slides on every antibody on our menu (), we have chosen to use a 3-prong method: 1. we

[Histonet] Paraffin blocks

How is everyone disposing of paraffin blocks regular trash or biohazard containers ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Paraffin blocks

We are currently using biohazard containers. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mro...@sfmc.net http://www.sfmc.net -Original Message- From:

RE: [Histonet] Paraffin blocks

In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles

[Histonet] RE: Cytokeratin AE1/AE3

What are you using for HIER? Citrate buffer? What pH? I use a low pH citrate buffer at 95C for 20 minutes and get good results. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508

[Histonet] Baylor University Medical Center Job Openings

Good Afternoon. I just wanted to let everyone know that Baylor University Medical Center Dallas has 2 job opening for Histotechnician Sr. One of my current technicians is moving into our new Research position leaving his position open. We also have been allowed to change 2 part time

[Histonet] RE: Cytokeratin AE1/AE3

Extra-follicular reticulm cells contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation

RE: [Histonet] Paraffin blocks

I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? Thanks, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology

RE: [Histonet] Paraffin blocks

Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though.Guess

RE: [Histonet] Paraffin blocks

You would think, especially when you see the amount of waste a tech can produce on high volume days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. Regards, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West

[Histonet] RE: Cytokeratin AE1/AE3

Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] Paraffin blocks

I have been following this stream of conversation and thought I might jump in. Paraffin blocks and microtomy waste are NOT biohazard waste. The process of fixation and tissue processing makes the tissue acceptable for general waste disposal. You do have to address the issue of PHI on the

RE: [Histonet] Paraffin blocks

Ours are incinerated because of the PHI. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, July 10, 2014 4:56 PM To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet

RE: [Histonet] Paraffin blocks

Interesting point. Thank you for chiming in on this Mr. Desalvo Best, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.commailto:vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From: WILLIAM DESALVO

RE: [Histonet] Paraffin blocks

Our facility handles paraffin shavings and blocks as Biological Waste which is different from Biohazardous waste. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, July 10,