Lately we are experiencing an issue where the tissue sections are coming off of
the slide during the staining process. We have always used positive charged
slides and have not experienced this problem on a routine case. We do not fix
the slides but allow them to air dry for Immunology.
I've been doing this stain for years and the protocol that I use came from John
Kiernan (Histonet Archives). If you use Sirius Red F3B (CI 35782) the stain
will be good for up to 3 years. Don't use a dye that does not have this CI #.
I have used the stain over and over and it always produces
Are you using pre-cut control slides?
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Vickroy, Jim
[vickroy@mhsil.com]
Sent: Tuesday, September 02, 2014 10:51 AM
To:
Greetings
I am a regular reader of this list, but from a peripheral setting.
I work in an electron microscopy certificate program. Our students are prepared
to do either or both certificates in EM, biological and materials.
During their biological training, students are instructed in thin
My opinion is that it is a good idea because many hospitals are shutting down
their EM department because of a lack of skilled workers.
So the more skills they have the better for them.
Let me know if any of your students want to move to Houston Texas.
Felton Nails
Texas Children's Hospital
Jon,
It is not a bad idea. Ideally a person going into the field would have a good
formal education in the field. However, 99.9% of the people working in the
field did not go through a formal program, but learned on the job. Therefore, a
person who was exposed to ANY formal education in
I agree with Tim the more you know the better off you are and if you have some
skills in Histology and can do EM that is a plus. Any form of molecular is a
plus now and many companies do not even require certification in any field as
long as you have the science and background to do the work.
I agree with Tim. Any additional skills in histology will make them more
marketable. EM and paraffin microtomy are very similar in principle.
As of yet an AA/A is not required to take the HT (ASCP) certification
exam. Route 1 states graduation from a NAACLS accredited program and
there are
Does anyone have any experience on which slides work better for frozen sections
and immunofluorescence stains..adhesive coated or positive charged?
Does anyone else find the selection of a proper slide confusing or is it just
my age? I understand the basis behind hydrophobic, hydrophilic,
Please take me out of the mail list.
Thank you
Sent from my Verizon Wireless 4G LTE smartphone
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I have used the CAP published 'Quality Management in Anatomic Pathology' and it
has been very helpful with this:
For a well-characterized antibody with a limited spectrum of antigenic
targets, like chromogranin, or prostate specific antigen, the validation can be
limited. A panel of 10
Hi Jim,
You mention that the OCT seems a little softer.
Could it be that the sections are thicker than you usually produce. Check the
Frozen section HE to check this.
If sections are thicker they will tend to detach from the slides.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,
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