[Histonet] Intestinal mucus -- Carnoy or what?

Hello! What is the best way to fix intestinal samples in order to preserve the mucus (and the embedded bacteria)? I was recommended Carnoy's, but are there any alternatives... perhaps without chloroform? With best regards, Mikael ___ Histonet

[Histonet] (no subject)

Hi all Is anyone of you using a an alternative for the DAKO-ARK kit. In my opinion it is good, but rather expensive. Best regards, Joost Bruijntjes ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] embedding of insects in historesin (Technovit 7100)

Hallo, are there any experiences in embedding of insects in historesin (GMA or Glycolmethacrylat (Technovit 7100))? I have tried Technovit after fixing in Formaldehyd with poor results. The specimen tends to fail out of the block. There is no connection between the cuticula

[Histonet] sectioning bee's heads and mites

___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Intestinal mucus -- Carnoy or what? (and: dry methanol??)

Oh, sorry, I forgot one additional question regarding Carnoy's: in the protocols I found, Carnoy's is prepared using DRY methanol. Sorry for my ignorance, but does this mean that methanol has to be specifically treated to remove any hygroscopic etc water? Note that this is specifically

RE: [Histonet] embedding of insects in historesin (Technovit 7100)

First of all, this sounds like a Damien Laudier question. Damien, I hope you can help this guy. Secondly, do you have to use plastic embedding? I was successful with paraffin embedding. I was able to section various small insects and also bees. It takes some patience and soaking in mollifex or

[Histonet] Thermo Scientific Richard-Allan Mark-it dyes

I already contacted them about this, but I thought that all of you might be faster: Would anyone happen to know if these dyes, if used to mark mouse bone/tissue around said bone at necropsy, would survive decalcification? We're trying to facilitate orientation of OA mouse knees during embedding

RE: [Histonet] embedding of insects in historesin (Technovit 7100)

Hallo, Andi G. Thanks for your reply. Normally I use paraffine for embedding insects after dehydration and using N Butylalkohol with sucess. In my opinion it is absolutly necessary to avoid any rests of water and to cut cool. But this technique is limited to sections of 3-4

[Histonet] Use of Polar Heat Pad inside Microwave Processor

I need a good, reliable company to buy from. This pad is not cheap. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499

Re: [Histonet] Use of Polar Heat Pad inside Microwave Processor

http://www.instructables.com/id/Microwave-Heating-Pad/ Like this? On Fri, Nov 21, 2014 at 12:21 PM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: I need a good, reliable company to buy from. This pad is not cheap. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology

[Histonet] Fast Green

Hey there, does anyone have a good method to de-stain Fast green? The slides need to be re-stained with HE. Thanks. Rebecca Berger, HT(ASCP)CM Research Technician Division of Orthopedic Surgery The Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC904

[Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor

[Histonet] lab math: ihc dilution

To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Recycler

We have a CBG Biotech recycler. We recycle Americlear, xylene substitute. How does everyone dispose of the waste alcohol and waste paraffin? Thanks and have a great weekend! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph

RE: [Histonet] Specimen numbering systems

Numeric for the Specimen and Alpha for the Block. Joelle Weaver MAOM, HTL (ASCP) QIHC From: donna.wil...@baylorhealth.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 21 Nov 2014 19:02:56 + Subject: [Histonet] Specimen numbering systems Can large facilities of

[Histonet] RE: Specimen numbering systems

Also, How are you specimens identified on your Surgical Requisition, 1,2,3 or A, B, C. Do you use the same identifiers in your LIS and Surgery. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465

[Histonet] RE: lab math: ihc dilution

Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of

RE: [Histonet] Specimen numbering systems

Same here. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Children's Hospital, Los Angeles -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Friday, November 21, 2014 11:30 AM

[Histonet] RE: Recycler

The waste alcohol is disposed of with the rest of our waste chemicals in a 55 gallon drum that picked is up by a licensed waste hauler. The paraffin is also picked up. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office

[Histonet] RE: lab math: ihc dilution

Sheryl I will add another bit of information. That final antibody protein concentration is very low. Most of our antibodies (around 60 to 70%) will be in the range of 1 to 10 ug/ml. We do have some antibodies that can have some very low protein concentration though such as neurofilament.

[Histonet] RE: lab math: ihc dilution

Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not .5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 Elizabeth Chlipala l...@premierlab.com 21/11/2014 3:35 PM Sheryl Here is how I

RE: [Histonet] RE: lab math: ihc dilution

Kathleen I have a final volume of 10 mls not 1 ml in the equation I wrote. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.commailto:l...@premierlab.com

RE: [Histonet] RE: lab math: ihc dilution

When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml. Therefore the dilution would be closer to 1:2000. I would usually divide the 1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate . Tom T -Original Message- From:

RE: [Histonet] RE: lab math: ihc dilution

You are right I got it wrong, see no matter how many time you run the math, you still come up with problems Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com

[Histonet] RE: Specimen numbering systems

We are currently using Numeric for the specimen (or part) and Alpha for the block. I don't like it; we frequently have 1Z blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director,

[Histonet] RE: Specimen numbering systems

We do Alpha numeric so A1,A2 but our samples also get barcodes so we have a barcode ID that picks up whatever you designate it to and it is AAA001234 (designating a block) the slides then become AAA001234 0001, AAA001234 0002 etc. ties it back to the block and uniquely identified (called

RE: [Histonet] RE: lab math: ihc dilution

You are so right-that's why I always try to double check everything even my spellink. Tom T -Original Message- From: Elizabeth Chlipala [mailto:l...@premierlab.com] Sent: Friday, November 21, 2014 12:05 PM To: Truscott, Tom; 'histonet@lists.utsouthwestern.edu'

Re: [Histonet] Specimen numbering systems

We use a numeric for the case number starting with the year 14-SR- and an alpha and number for blocs with the case.  A1, A2, A3 so it looks like 14-SR- A1 and the next A block is A2 and so on with as many alpha designations as needed.  Last week we had one surgical case with A through

Re: [Histonet] Fast Green

Try 70% ETOH, leave the slides to soak for a good while. If that doesn't work after a couple of hours, try 1% HCl in 70% ETOH. Potassium permanganate followed by oxalic acid takes out just about anything, in my experience, but it's pretty harsh (1% aq potassium permanganate, 2% aq

[Histonet] Reference to microtome micrometer thickness

Hello Histonetters, how will we verify the thickness of a paraffin section in a slide. Do we have a reference regarding on how to measure the thickness. I based the thickness of my section thru the mcirometer on the rotary microtome but one of our reveiwers does not believed that we are cutting

[Histonet] Re: lab math: ihc dilution

Here's how I approach this problem. Convert all concentrations into the same units. I prefer to use whichever the final concentration unit is, so it looks like this: 1.56 mg/ml = 1560 ug/ml You have 1560 ug/ml and you need 0.8 ug/ml Divide 1560 by 0.8 = 1950. So your final dilution is 1:1950.

[Histonet] Help with muscle

Hello All, I hope I am not asking a dumb question. As I know that everyone on the histonet is very knowledgeable I was hoping to get some suggestions regarding processing tissue for neuromuscular junction staining. We are a neuro research lab and want to quantify neuromuscular junction on

RE: [Histonet] Specimen numbering systems

I am doing the same of Joelle Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |  Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099