Immunostaining for GFAP has been the definitive method for astrocyte cytoplasm
for 30 years, and good primary antibodies have been around for at least 20
years. The distinction between different kinds of astrocyte is made by staining
with serial dilutions of the primary antiserum. Reactive astro
I used to be the Queen of ORO in my lab at the University of Arizona. I had
some of the fattiest livers that could be possible from different research
projects. I never had a problem with the livers staying on the slide. I used
Epic coated slides or Stat Lab slides and the protocol was from Frei
Fixation must be adequate before decalcifying. 12-24h in Bouin is OK. My
favourite formic decalcifier is that of Clark (1954) Am. J. Clin. Path. 24:
1113-1116. It's a buffer (pH2.0) made by mixing 90% formic acid 250ml, water
750 ml and sodium formate (anhydrous) 34g. Keeps for ever. Check the r
I was wondering if anyone could recommend an antibody (and best dilution) which
is more effective than GFAP in staining both resting and active astrocytes in
human brain please.
Thanks
STEPHEN KUM JEW | Senior Technical Officer
Discipline of Pathology | School of Medical Sciences
THE UNIVERSIT
I Know fatty tissue is such a pain to cut. Bryan Llewellyn gave some really
good techniques. Im going to try them out myself :)
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w:
www.schn.health.nsw.gov.au
Cnr Hawkesb
+1 to Linda, but I have found no difference on overnight vs multiple days.
Fatty liver is hard to do on all counts! It is tough enough sometimes to get a
decent section on the slide.
Thanks for the other suggestions, certainly something I would try in the future
Yours
Caroline
Caroline Miller
Hi Tryrone
A Mass Trichrome stain will stain up the collagen in the diseased kidney. If
the tissue has already been fixed in Bouin's Solution you can omit the picric
acid step. Another good stain is the Curtis's Van Gieson Stain. Ive sent you
the protocol for botht he stains. Hope it helps.
Usually with the fatty tissues, I pick them up on superfrost slides and let it
air dry for 2-3 days at room temperature and then perform the ORO stains. So
far they seem to stay on.
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.n
From: Carol Fields
Sent: Wednesday, April 15, 2015 2:48 PM
To: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: Cost of a Slide in LA
Hi Netters,
Could someone tell me what the cost of a slide would be in the Los Angeles
area? Also what labs charge in this area to make a slide?
I realize I
From: Carol Fields
Sent: Wednesday, April 15, 2015 2:48 PM
To: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: Cost of a Slide in LA
Hi Netters,
Could someone tell me what the cost of a slide would be in the Los Angeles
area? Also what labs charge in this area to make a slide?
I realize I
Watch the liver, it will change color as the blood is washed out. Are you
perfusing first with PBS to rinse the blood out, if you start with formalin
small blood vessels and capillaries can become blocked by the blood that has
coagulated when exposed to the formalin. We actually flush with 10%
Hello,
Some questions regarding Bouin's solution.
I was told, back when I was doing my PhD and new very little, that I should
fix my fish in Bouin's as it will decalsify the bones. Well, Bouin's fixed
fish were easier to cut than PFA fixed fish... but I read today that by
adding formic acid the d
Hi Yves & Histonet
It is certainly a good sign if limbs etc are stiff after perfusion, but maybe
not a guarantee that the target organ is perfect given the short perfusions you
describe. Definitely, if I don't see stiffness I worry, check for a torn aortic
arch (you are doing it transcardially,
Thank you for suggestions for softening beak.
Yes, I actually processed the whole head for decalcifying first and then place
the sample in 10% KOH for 30 mins. It was my first attempt to process beak
(including a whole head) thus, i did not know i should have processed softening
beak first.
An
Hi Histonetters!
So how is your week going? I cut out of work early yesterday and headed
over to the beach to watch the launch of the SpaceX Falcon 9 Rocket.
It was amazing
I've been promising myself for years that I would go over and watch a launch
at the beach. Living in Orlando I rarely
There are a couple of old techniques you might try.
1. Smear some Mayer's egg albumen on a slide as you would normally do,
i.e. very thinly, then hold it in a flame until it smokes. Allow to cool
and pick up the section. Allow to drain well.
2. Same as 1, but place the slide with the picked u
We are having difficulty with a particulate set of very, very fatty mouse
livers. The normal livers from this set stay on the slides the fatty livers
fall off. We have used different types of charged slides and we have even
tried to drench the charged slides in Stay-On, dry them and then put t
Hello to all in histoland. Does any one have a competency evaluation for a
Pathologist Assistant that they would be willing to share. Any help in this
would be greatly appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)
CONFIDENTIAL
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