[Histonet] Direct Hire Histotech Needed in Santa Maria, CA

Hello, I have a full time opening for a permanent/long term Histotech in the Santa Maria, CA area. Please contact me if you would like more details. Thank you! Check out our article in I4Biz June's Addition Allied Search Partners connecting Labs to Employees

Re: [Histonet] Coverslipping mystery

Adam: Just guessing, but I think the mystery is caused by how fluid the DPX is. Guessing again, but it probably is more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.René On Thursday, July 9, 2015 9:40 AM, Adam Boanas

Re: [Histonet] Coverslipping mystery

I am not familiar with DPX, but is there a compatibility issue between it and the slide clearant you are using? Tresa -Original Message- From: Adam Boanas [mailto:a.boa...@epistem.co.uk] Sent: Thursday, July 09, 2015 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

Re: [Histonet] subbing positively charged slides

Chrome-gelatin cannot be expected to stick well to a glass surface that has been modified by adding aminopropyl (or similar) groups to its silicate ions. See http://publish.uwo.ca/~jkiernan/adhesivs.htm. John Kiernan London, Canada = = = On 09/07/15, Travers, Susan traver...@osu.edu wrote:

Re: [Histonet] subbing positively charged slides

Why would you want to do this? You could buy plain slides and sub them yourself. Geoff On 7/9/2015 8:45 AM, Travers, Susan wrote: Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just

Re: [Histonet] Coverslipping mystery

Look in your manual, near the back. There should be mountant type calibration settings for yo to adjust the machine to. That, hopefully will solve your issue! Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message-

[Histonet] 2 Open Positions in Little Rock Arkansas

WE currently have two open positions for either registered HT or HTL. Registry must be current and some experience. We are a heavily computerized Histology Laboratory and will require the persons applying to be comfortable with computers. We are a routine Histology Laboratory at the

[Histonet] Medica ELGA DV 25

Does anyone use the DI water system called Medica by Eqova?  We just recently got 2 in our lab and was wondering if anyone had an SOP you'd like to share?  Can you tell my what daily QC needs to be done with it?  Water tested?  Thanks! ___ Histonet

[Histonet] Roche B-gal Staining Kit

Hello, I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse sections. Another histologist recommended post-fixing in a glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit instructions. This has me worried that there may be other things

Re: [Histonet] Specimen loss

Steve McClain writes: In UPMC protocol They wrap all specimens, ink the tissue with eosin (erythrosine) at the grossing bench and cut two levels at 40 microns. Do NOT use eosin for this purpose. It's highly fluorescent, and will interfere with any method (such as FISH) that relies on

Re: [Histonet] Coverslipping mystery

What type of clearing agent are you using? The aliphatic hydrocarbons are not compatible with all mounting media. From: Adam Boanas a.boa...@epistem.co.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 07/09/2015 06:16 AM Subject:[Histonet]

[Histonet] Field Histology Opportunity

Hello Histonet! We have recently had a new position open with a world leading histology manufacturer. Our client is looking for a field based Histology Support Specialist to cover a region and provide support to their clients. The ideal candidate will have a background in histology and

[Histonet] histotechs needed

Hi all! I just had a couple of retirements (20+ years for both) and I am looking for 2 histotechs to come live and work in beautiful Santa Barbara, CA. If you are interested or know anyone who might be, please give me a shout! Thank you, Noëlle Noëlle Linke, MS, HTL(ASCP) QIHC Manager,

[Histonet] Specimens lost in process

Steven, Here are some thought I have on each of these from the perspective of managing a large GI lab: 1.  I have only had one specimen that was lost by the grossers, and it wasn't lost.  The cassette went into the bin with the specimen containers for the day.  This is where I found the

[Histonet] reply

Replying to confirmation Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

Ms. Blazek, To clarify the matter, I read the cited paper by SR Owens, and I interpret those data differently, and in my opinion, the 7% figure for lost specimens is incorrectly quoted. The majority of his cases where there was a difference between the number of fragments submitted and the

Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

As one of the lead participants in the cited study We never lost specimens. Did we have fewer than was noted on the casette from gross, yes. Could we attribute that to mucoid samples, yes and no. We counted everything, even dust like particles, hence the 6% fewer on the slide. Keeping in

[Histonet] subbing positively charged slides

Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide...

[Histonet] RELIA HOT HISTOLOGY JOB ALERT HISTOLOGY SUPERVISOR - DAYS !!! - DALLAS TEXAS A RELIA EXCLUSIVE!!!

Hi Histonetters!! I hope you are having a great day. I have an exciting opportunity to share- RELIA has been engaged on an EXCLUSIVE search for a Histology Supervisor for a leading lab in the Dallas area. ASCP certification and experience managing a group of 30+ people is required. This is a

[Histonet] Coverslipping mystery

Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that

Re: [Histonet] AFB contaminant

We had some a few years ago and thought it was contaminating bacteria, but turned out to be dirty water from the di tap. We had our plumbers take it apart and clean everything. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies

[Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?  My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.  Any

Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax

[Histonet] AFB contaminant

Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St

Re: [Histonet] AFB contaminant

Hi, This may be AFB contamination from tap water. The organisms grow in the end of cold water taps. You should take the taps apart and thoroughly descale and scrub out. The organisms may be somewhat longer and thicker than TB, and may be quite distinctly beaded. Bryan Llewellyn Abbott,

[Histonet] XGal staining

 Dear All, and Anna.I am confused about the recommendation of post fixation (stained slides?) in glutaraldehyde-foormaldehyde solution. According many protocols and my experience the fresh tissues  should be fixed in in glutaraldehyde -formalin solution that is mproved the tissue morphology and

Re: [Histonet] AFB contaminant

most likely a fungus ball, confirm with a PAS Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and

[Histonet] Gross Room QC sheet

Does anyone have a Gross Room QC sheet you'd like to share?  Thanks so much! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Ventana antibody - H pylori

We have excellent and very clean results with the VMS H. Pylori on our Ultras. I can shoot you our protocol if you're interested. Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX:

[Histonet] Histology phantoms?

Hi Histonet! Happy 'almost' Friday :) Can I ask if anyone out there has ever put phantoms or other fiduciary markings into their paraffin blocks to help with registering serial sectioned images or other downstream processes? thanks in advance! yours, mills -- Caroline Miller (mills)

Re: [Histonet] Ventana antibody - H pylori

Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent:

[Histonet] HISTO POSTION

We are hiring full time Histologist with mini 5 year experience in all aspects of histology ,especially IHC and Breast Panels. Behnaz Sohrab Regional Manager Anatomic pathology behnaz.soh...@ah.orgmailto:behnaz.soh...@ah.org 323-268-5000 Ext.1711 323-265-5086 FAX