[Histonet] Received date.

Hi Terri - That list is specific to New York State, no other state is so restrictive, and it doesn't cite 'received date' as a requirement. If you've got all that other stuff-- which is generally included on reagent manufacture labels with minor exception-- I can't think of what value a recei

Re: [Histonet] changing tissue processors

I would suggest by cassette numbers. It will take some data collection, but once you settle on the number of cassettes, then you will have the process in control. The actual number will depend on the tissue types placed in the cassettes and the amount of blood, lipids and other elements extracte

Re: [Histonet] changing tissue processors

Nirmala, We use the number of blocks to guide on how often to change the processors. We experimented with how many blocks we could get away with and then backed the number up by 50 blocks to be sure. VIP5/6 for us is currently 700 blocks. Beyond that, tissues are not thoroughly processed. We used t

Re: [Histonet] IgG4

We are using the MRQ-44 from Cell Marque. From: Cartun, Richard via Histonet [histonet@lists.utsouthwestern.edu] Sent: Monday, January 11, 2016 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG4 I am curious to hear which clone people

[Histonet] PR antibody

Hello Histonetters- Does anyone use PR clone 1E2 for the ER/PR receptors? Name of a vendor? Thanks in advance. madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwester

[Histonet] IgG4

I am curious to hear which clone people are using to detect IgG4-positive plasma cells in FFPE tissue. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assi

[Histonet] changing tissue processors

Hi Histonetter, Does any one have guidelines, which indicates how often to change the tissue processors - Cassette Volume run, Make of processors etc Thank you Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 632

Re: [Histonet] mouse femur decal and paraffin embedding protocol

James We use 10% Formic Acid to decal mouse femur and tibia - The rear legs are removed at the hip with a pair of rongeurs the skin is removed to the ankle, we allow the knee joints to fix for 48 hours, they need to be in a normal degree of flexion, meaning you want them in a container that d

[Histonet] mouse femur decal and paraffin embedding protocol

Dear Histonet Colleagues, I hope the holiday season has been good to all. We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to

Re: [Histonet] received dates no longer required

As of January 2016, the updated NY State Dept of Health standards still require received date, and date the material is placed in service or opened: Reagents Sustaining Standard of Practice 4 (REAG S4): Inventory Control There shall be an inventory control system for supplies. This system should

[Histonet] dry biopsie questions

Start with retrieval from biopsy what are they doing with the tissue immediately after removal from body Second, processing times are very important to. Our institution uses a short program with watching the time in formalin to be not less than 6 hours. (This is For tissue that is 3 mm or les

[Histonet] Pancytokeratin (AE1/AE3) Protocol

All of a sudden our AE1 is no longer good for the pathologists. Anyone willing to share their protocol for Ventana Benchmark XT or Ultra? I have "tweaked" this protocol so many times. It amazes me how often stains are great during optimization and validation, then months later, everyone hates

[Histonet] Antigen retrieval question. and a bit more

Hello 'netters, hope you all had a good weekend. We are having some difficulties with the pressure cooker chambers we use for antigen retrieval. What different methodologies would be recommended? If we went to a different methodology for retrieval, would each antigen have to be revalidated? Ho

Re: [Histonet] Diapath Histology Machines

Hi Histonet Anyone have experience about the Diapath Histology Machines . Automatic Tissue Processor For Histology. Specimen., DONATELLO, open system 52PRO03 . Semi-automatic microtome Galileo SEMI - Diapath . CANOV

[Histonet] GI biopsies processing

Hi All, Can anyone share their processing set up for GI biopsies? No matter what I do, our tissue seems dry. Any input would be great. Thanks in advance, Mehndi Helgren Dominion Pathology Labs. ___ Histonet mailing list Histonet@lists.utsouthwestern

[Histonet] Histology Position at Univ. of Michigan

Applications being accepted for a full time research histotechnologist at the Univ. of Michigan in Ann Arbor. Please review posting #120103 at www.umjobs.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mail

[Histonet] Obtaining Controls

Does anyone know of any references or specific guidelines (CAP or CLIA) regarding the use and collection of control tissue from patient samples? TIA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/lis