[Histonet] PAP staining quality

2016-02-04 Thread Beth Cox via Histonet
Hi Charles, A couple things to check on: 1. The first concern I would have is your EA stain. Poor EA staining will give too much orange staining and pale other counterstains (making them look aged).What brand are you using? Have you changed brands? Is your EA close to the expiration dat

[Histonet] PPE/ Hair Regulations

2016-02-04 Thread Amos Brooks via Histonet
If it is actually getting caught in things or falling in waterbaths or embedding centers and such, then it needs to be tied back. The same is true of food service. You can't risk contamination. If it is under control though, leave it alone. Don't try to use safety to enforce your personal bias of h

Re: [Histonet] clearing time

2016-02-04 Thread Elizabeth Chlipala via Histonet
Mohamed For larger samples we have ranges from 1.5 hours to 6 hours a station. Anytime we have larger samples we always utilize three absolute alcohols and three xylenes, for your samples I would try 1.5 to 2 hours per station to start. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier La

Re: [Histonet] Organizing IHC

2016-02-04 Thread Elizabeth Chlipala via Histonet
Lesley We have a folder for each antibody, this folder contains all of the information on that antibody. It includes all of our protocol development information, the slides from protocol development in the flat plastic holders, spec sheets, internet searches, publications and references. All

[Histonet] clearing time

2016-02-04 Thread mohamed abd el razik via Histonet
Dear all what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ? thanks Mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Organizing IHC

2016-02-04 Thread Lesley Bechtold via Histonet
Good Afternoon, I have a question and I'm hoping for some creative answers. How do other labs track their IHC? Right now, we have 3 large binders with the paper work-up sheets and package information filed alphabetically. We have nothing electronic other than an Excel spreadsheet with optimi

[Histonet] Medica DI water system with Symphony

2016-02-04 Thread Histology Technician via Histonet
Just curious to see if any of you have a Medica DI water system with your symphony?  I was wondering what QA/QC I need to do to it and an SOP.  I'm the only lab in my building, so if I have to test the water, I'd have to take it to a nearby hospital.  Thanks!  This is the first DI water system

[Histonet] Pay Scale

2016-02-04 Thread Elaine allison Hoffman via Histonet
Greetings all, I need to get an update on the most recent pay scale for a certified histotech vs a non-certified histotech in Northern Ohio region.  Also other various descriptions as well, like starting pay, experienced histotech pay, manager/supervisor pay, HT vs HTL, etc.Any input would be ap

Re: [Histonet] Pap stain quality

2016-02-04 Thread Bob Richmond via Histonet
Charles Riley HT(ASCP)CM, Histopathology Coordinator/ Mohs, Doctors Pathology Services, Dover DE asks: >>Not sure if anyone out there would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and looking aged. If you have any idea for causes I appreciat

[Histonet] Slide printer question

2016-02-04 Thread Sanders, Jeanine (CDC/OID/NCEZID) via Histonet
All, What is the fastest slide printer out there that can print large numbers of slides on any one block and allow you to continuously add the new cases back to back without delay. Thanks, Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE M

Re: [Histonet] PAP stain quality

2016-02-04 Thread Jamal Rowaihi via Histonet
You need to review the slides fixation and what chemicals you are using Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: Charles Riley via Histonet Date: 2/4/2016 5:50 PM (GMT+03:00) To: histonet@li

[Histonet] PAP stain quality

2016-02-04 Thread Charles Riley via Histonet
Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover