Re: [Histonet] Nuclear Bubbling

I don't have your answer Jim, yet I can vouch for your experience as a supervisor since you were my boss for about two years. Hopefully you can get it resolved because it can be very frustrating as the tech. On Tuesday, February 16, 2016, Vickroy, James via Histonet <

Re: [Histonet] Nuclear Bubbling

For the record please note that I have over thirty-six years experience working in a Histology lab. I have been a supervisor or manager of a hospital and clinic histology department for at least 25 years. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South

[Histonet] nuclear bubbling

Dear James: Two chained events take place on your issue: 1-sections not entirely drained,water remains even minimally under sections; 2- you put these sections in the oven, the heat literally "explodes" the nuclear bubbles and creates a hole with no cromatin to stain. My kind regards, Carlos

Re: [Histonet] Nuclear Bubbling

Great, I agree  Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: "Manfre, Philip via Histonet" Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa

Re: [Histonet] Nuclear Bubbling

Sort of a rude response to someone looking for help. -Original Message- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I

Re: [Histonet] Nuclear Bubbling

There is no need to be rude. He has tried the drying option and is still having nuclear bubbling. He is exploring other possible issues. You would see this if you read the email in its entirety. From: Rene J Buesa via Histonet To: "Vickroy, James"

[Histonet] (CLRW) Clinical Laboratory Reagent Water

Deanne, I purchase 5 liter cubes from Mercedes Medical. They can provide test results on each batch of water and fax to you. I did have a CLIA inspector advise to document daily on the water as is written on the cube for clarity of water, no sediments or signs of contamination. Think 5 Liters

Re: [Histonet] Nuclear Bubbling

If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the

[Histonet] Sakura Pre-Processing Fixative (7117) for X120 Processors

Hello All, I manage a histology Dermpath lab (We only process skins) and we use Sakura X120 Processors. We have been doing excellent with the use of our x120 processors that allowed use to have slides on the Pathologist desk within 5 hours from the time we received the specimens. That said,

[Histonet] Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST

Hello All, Do you post-process your scientific images? Typical tools for common adjustments include Photoshop and ImageJ, but they aren’t the best answer. You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST – “Best Practices for Post-Processing of Scientific Images”

[Histonet] Nuclear Bubbling

Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.

Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water

Deanne, If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the

[Histonet] MICROTOME KNIFE SHARPENING

Hi all... Hoping you might be answer a couple of questions that I have. 1) Does anyone use a microtome knife sharpening kit made by Pathco? If so, 2) How well does it sharpen the blades? 3) Is there any issue on attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets