Re: [Histonet] Nuclear Bubbling

2016-02-17 Thread Hoekert, Willem via Histonet
Could it be due to incomplete deparaffinization? Willem Van: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu] Verzonden: dinsdag 16 februari 2016 18:10 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Nuclear Bubbling Struggli

[Histonet] keratohyalin granules

2016-02-17 Thread Kalleberg, Kristopher via Histonet
Hello All, Does anyone have experience staining the keratohyalin granules in skin with different types of hematoxylins? I am currently looking at keratohyalin granules and staining with Harris hematoxylin and there seems to be little staining and not as prominent as I would have expected. Won

[Histonet] New employees

2016-02-17 Thread Elaine allison Hoffman via Histonet
Greetings all, Does anyone have a new employee check-off worksheet that they would be willing to share for histotech's?Thanks in advance! Elaine Hoffman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/l

[Histonet] Bar Code and Tracking

2016-02-17 Thread Abbott, Tanya via Histonet
Hello, I manage a small Pathology lab where we generate approx. 80-140 blocks for Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto assistant. I am curious if there are any smaller labs out there that

Re: [Histonet] Nuclear Bubbling

2016-02-17 Thread Aubrey Wanner via Histonet
NSH has an archived webinar: Nuclear Bubbling: It's About Time" created by Peggy Wenk. The webinar talks about several causes of nuclear bubbling, changes that can be made to reduce this problem, and, for really bad cases, Peggy talks about how to dry the slide differently, reprocess tissue or

Re: [Histonet] Nuclear Bubbling

2016-02-17 Thread deGuzman, Jose R via Histonet
Jim, Are you drying the slides in an oven before the heat stage on the Prisma? If you use an oven, what is the temperature setting and for how long are slides kept in? Carson and Hladik recommends 60 degrees for less than 1 hour. You mentioned, recycled formalin, what is the pH of the formalin?

[Histonet] RELIA HOT Histology Jobs Alert! 2-17-2016 Happy Hump Day!

2016-02-17 Thread Pam Barker via Histonet
Hello Histopeeps! Happy Hump Day! We are halfway to the weekend! We GOT This!! Since it's mid-week and my phone is ringing off the hook with new opportunities I wanted to do a quick post to tell you about the positions that I am working on and am MOST excited about. Why am I excited about these po

[Histonet] Error Prevention:

2016-02-17 Thread Jb via Histonet
Does anyone have a policy that they are willing to share regarding tech errors, error prevention, etc? How do you document, retrain, write up, and terminate employees for errors? What is considered severe problems for termination, etc. I know it is up to the manager, I want to know what othe

[Histonet] Sequenza Immunostaining Racks

2016-02-17 Thread Carlos Genty via Histonet
Good Day Everyone, I am looking to obtain used Thermo Sequenza Immunostaining Racks (about 10 or so). Does anyone have any that they would be willing to part with? Please feel free to contact me directly. Thanks in advance! Carlos Carlos Genty Critical X Solutions, LLC cge...@criticalxso