Thanks Bob. Somebody else mentioned her name also so I'll be sure to reach
out to her.
THE expert on the Movat pentachrome stain is Akemi Allison, a very senior
histotechnologist who's published about it. I can't remember if she's on
Histonet, but you can contact her on Facebook - I hear from her
Hi Terri,
I have used Poly Scientific kits in the past and agree that they are
fabulous. If I were still in anatomic pathology I would wholeheartedly
agree with you. However, I'm now in a research facility and the kit is
simply cost prohibitive. Fortunately, I do now have time for homemade
(a true
Thanks for sharing. It's very helpful. Where do you purchase your safran du
gatinais/saffron from? At this point I'm ready to start growing my own
Crocus sativus.
On Thu, Sep 1, 2016 at 12:35 PM, Amos Brooks wrote:
> Hi,
> I use Lugol's Iodine all the time for this. It works just fine. I do
Hi,
I use Lugol's Iodine all the time for this. It works just fine. I do
purchase certain chemicals from manufacturers like Lugol's Iodine which I
get from EMS. Most of the chemicals I make up myself from powder though. I
have shared the procedure with you via Google Docs. I hope it helps.
Am
Hello histoland
We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the
last few months our pathologists have been noticing "junk" that is staining
with this antibody. It seems to be on the tissue more than in the tissue and
its not on all slides. WE have tried changin
We ran into this situation with TJC. We had a very limited check off sheet.
In effort to standardize processes across the laboratory we adopted a program
very similar to the NY facility whose program is referenced in the TJC leading
practice library. It is very rigorous and time consuming but
Nirmala, Histotechs are not required to have competency assessment under CLIA
regulations because, as you note, they do not interpret any tests or report any
results. However, if they do grossing then they do require high complexity
competency assessment.
Having said that, if there is some conc
Angela Lamberth at the La Jolla [California] Institute for Allergy &
Immunology asks:
>>I'm gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I've found a
protocol used by the Children?s Hospital of Philadelphia Pathology Cor
Nirmala, Histotechs are not required to have competency assessment under CLIA
regulations because, as you note, they do not interpret any tests or report any
results. However, if they do grossing then they do require high complexity
competency assessment.
Having said that, if there is some conc
If you are not that concerned over the alcian blue portion of the movats you
can perform an elastic trichrome - that's what we do here. Mordant like you
would in Bouins, and rather than stain with weigerts hemastoxylin, stain with
the elastic portion of the VVG, differentiate like you would for
Just wondering why you are compelled to make all the reagents in house for the
Movat's stain. I have used the kit k042 from Poly Scientic with fabulous
results (and no, I have no gain from recommending them).
Their solutions are wonderfully labeled and can be replenished individually, or
by ord
Hi,
What is everyone doing about the Histology Staff competencies. I know
CLIA has several criteria for the med-tech competencies. Since the
Histotechs do not report out results, how is everyone implementing this
process. Any information is greatly appreciated.
Nirmala
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Angela, the Movat is a wonderful thing for showing loss of elastic fibers in
emphysema and cardiovascular cases. We ran a ton of them in training because
our lab/histo school was affiliated with the Texas Heart Institute.
Currently at the hospital I work at we use a Kit(Polyscientific) for the
Hello Histonetters
Was wondering if anyone would share their protocol for the above ABY we are
utilizing the Ventana Ultra and purchased the ABY 3rd party from Cell Marque I
would appreciate the help the insert doesn't have the standard protocol on it
like the rest :( just want to be safe.
Thank you for the suggestion. I'll ask around when I reconnect with people
at Long Beach this month.
On Thu, Sep 1, 2016 at 7:47 AM, Rene J Buesa wrote:
> Do you have any contacts at any old histology lab, i.e., one that has been
> in operation for more than 50 years? You may find there iodine c
Do you have any contacts at any old histology lab, i.e., one that has been in
operation for more than 50 years? You may find there iodine crystals and ask
for a few grams. I used to have a 500 g bottle at my lab (which began in
1947).René
On Thursday, September 1, 2016 10:39 AM, Angela Lam
I would prefer to not substitute anything yet the procedure calls for a
minimum of 2 grams of iodine which I simply cannot buy in the United States
thanks to new DEA regulations.
On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa wrote:
> Once you start substituting things in an original recipe, the o
Once you start substituting things in an original recipe, the outcome cannot be
expected to be what the original recipe was supposed to deliver.Iodine crystals
cannot be substituted by Lugol because, besides the iodine also contains its
salts. and alcohol. They are two completely different thing
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