I would also check the fixation.
Do the smears look air-dried. The larger nuclei, following air-drying do not
concentrate the Hx as much as prompt alcohol fixation resulting in paler
stained nuclei.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientis
Yep start at last alcohol in other processor, xylene & wax as usual.
If the tissues have remained covered in alcohol, you should not have a problem
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow,
We add a comment when the specimen handling is outside of 2013 CAP/ASCO
guidelines for breast or 2016 CAP/ASCO/ASCP guidelines for HER2 testing in
GEA cancers. These guidelines and the data supplements have some good
information. We usually have found that decal doesn't affect the IHC much
if not
Hello Histonetters,
A North Scottsdale Dermatology practice is seeking a part-time Histology
Technician. Two days a week, (approx 8-10 hours total) one day would be logging
in and grossing biopsy and excision specimens, the next day would be cutting
and staining. Currently no special stains are
Jason,
The only way is to validate for those conditions. You would need some
specimens, including known controls, handled and processed in those same
conditions and compare to samples processed under the acceptable conditions.
You can do it, it will just take some time.
Tim Morken
Patholog
To help this you could place the cassettes in the other processor in the last
100% for about 5 min, just to freshen them up. Then proceed with the remainder
of the process as usual.
FYI, this will be one of our discussion questions this year in our HTL program.
T
Toysha N. Mayer D.H.Sc., MBA
Does anyone know or can you point me in the right direction to some literature
about how to properly test for Her2Neu (IHC vs. FISH) on breast tumors if the
cold ischemia time is greater than 1 hour or if the formalin fixation times are
outside of the recommended time range? Also, what if the sp
Hi Histonetters!
How are you doing today?
I hope today and the rest of your week is absolutely fantastic.
I Have A Few Quick Questions For You
I am thinking of restarting a blog I was writing a few years ago. I really
hope that it will appeal to histology professionals at all levels.
I dont
Are the smears collected, fixed and treated as "usual" along ALL the steps?René
On Wednesday, April 19, 2017 12:54 PM, Charles Riley via Histonet
wrote:
I have been put in charge of figuring out why our pap stains are light on
the hematoxylin. Everything was filtered and used fresh as p
I have been put in charge of figuring out why our pap stains are light on
the hematoxylin. Everything was filtered and used fresh as per usual using
the same protocol we have used for the past two years.
If anyone has any suggestions as to how to fix this problem I would greatly
appreciate it. If
REMINDER: Survey will close at the end of this month. Respond now to be entered
into the drawing.
Hello Histonetters,
I am conducting a study to learn about your experiences in the laboratory with
microscope slides. The information gathered will help assist with product
development decisions
It has happened to us as well. I'm not sure where you work, but our processors
have alarms that go to security. So I have gotten a call on off hours to come
in and fix the problem or try to trouble shoot. Some of the newer processors
can be linked to your phone, etc.
-Original Message---
Another reason that IHC is used instead of IF is with IHC, one preserves the
ability to see tissue/cell morphology through Light Microscopy at the same time
as the visual IHC label. Morphology is difficult to see with IF, with the
exception of the fluorescein labeled area.
Terri L. Braud, HT(
Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!
Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet
> wrote:
>
> In 100% EthOL the tissues are completely "salvaged" and you can prepa
In 100% EthOL the tissues are completely "salvaged" and you can prepare the
program to continue the steps until melted paraffin.If there are delicate
tissue perhaps they will be "over-dried" but that is easily "compensated"
during microtomy.René
On Wednesday, April 19, 2017 9:01 AM, Lauren
Good Morning Histonet!
I wanted to take a moment to express my gratitude to all of my colleagues on
histonet. You are an invaluable resource in a world that is spread-out and I
treasure the ability to reach out to you when I have a problem.
Case and point, I have been struggling with a H&E pro
Hello Histoworld,
I came in this morning to find that the processor died halfway through process
last night. The tissues are in 100% ETOH exactly half point. We do have a back-
up processor. In your professional experiences, would these tissues be
salvageable? Could I create a new program on th
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