It's not safe to use any type of spray in a cryostat. It creates aerosols
of who knows what (tuberculosis) from potentially infectious patient
biopsies. I would not recommend it, just as a universal precaution.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego
There are non flammable sprays available. We got the recall notice and switched
our old inventory out. I found 3 different histology specific sprays. The
electronic industry offers some as well. Another option is just using LN2
.Jason HauserSenior Histology TechnicianThe South Bend ClinicSent
I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential
TB and regular masks for normal tissue.
Akemi Allison
Sent from my iPhone
> On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet
> wrote:
>
> I have to agree with Terri. I also believe that CAP is going to be
I have to agree with Terri. I also believe that CAP is going to be putting
in regulations forbidding the use of freeze sprays in cryostats soon (at
least I think I heard a rumor about this).
Anyway another useful item is liquid nitrogen if you really need a quick
freeze. Dip a brush into the
My question is why would you ever want to use an aerosol in the cryostat,
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or
goodness-knows-what?
We use a simple metal heat extractor (comes standard in our Leicas) and
Hello histonetters,
Please share this email with your colleagues.
I am in a job searching process in Chicago area to be close to my family as my
appointment in West Virginia University Research Corporation, Morgantown, WV is
coming to an end since my long-standing supervisor is closing her
Liquid Nitrogen guns.
Claire
From: Knutson, Deanne via Histonet
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Flammable Sprays in Cryostats
WARNING: This email appears to have originated outside of the UW
We received the same letter months ago. We changed to a non-flammable spray.
It is called STATFREEZE, we purchase it from STATLAB. The catalog number is
SL202/134a. Be careful if you order it because they also sell a product called
STATFREEZE that is flammable.
Valerie
We are purchasing two of their newer UV cryostats and they were adamant that we
use a non flammable freezing spray because it can damage the UV unit and have
potential for exploding.
Akemi Allison
Sent from my iPhone
> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet
> wrote:
>
>
I just received a letter from Leica Biosystems where they are prohibiting the
usage of flammable
freezing sprays in their cryostats. What are others using in their cryostats
to instantly freeze specimens?
Thank you.
Deanne Knutson
Supervisor
Anatomic Pathology
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