Re: [Histonet] FW: Microtome at home

2020-04-16 Thread E. Wayne Johnson via Histonet
"Taking control of the situation is key." It's very interesting to me that most of the responses have to do with institutionalized bureaucratic ideas (safety, insurance, liability, regulations) rather than how to get things done. I was thinking about how Virchow and Henle and other pioneers

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Mark Tarango via Histonet
I had heard that CLIA was relaxing things and is not requiring a new # to work from home right now. Best to check on the regulatory but FFPE isn't typically infectious. The ideal spot would in the garage and not the kitchen though. On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet <

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Roxana Robinson via Histonet
Agree. 8-10 microns cut just prior to staining. Roxana Robinson > On Apr 16, 2020, at 3:50 PM, raestask via Histonet > wrote: > > Should also be cut on the day they are to be stained.Rae Staskiewicz > HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone > Original message

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Roxana Robinson via Histonet
I do not agree with this in our current situation or actually any situation. There are quidelines in place with CLIA, OHSA and CAP for protecting not only the patient but also the employee. Whether research or not. Roxana Robinson > On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet >

Re: [Histonet] Microtome at home

2020-04-16 Thread Patsy Ruegg via Histonet
I have done it, but you are right, I had my own private business, not sure why it would be a problem, especially for research. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Patsy Ruegg via Histonet
I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Tony Henwood (SCHN) via Histonet
I agree, Slightly thicker makes the polarisation easier to see (personal experience). I would love to see a study comparing section thickness Vs polarisation characteristics. Anyone interested? (being a kids hospital we rarely see amyloidosis) Regards Tony Henwood JP, MSc, BAppSc,

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Morken, Timothy via Histonet
Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. Tim Morken Supervisor, Electron Microscopy/Neuromuscular

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Theresa Dalton via Histonet
I work at a Dermatology lab and our protocol for amyloid is 8-10 microns. Sent from my iPhone > On Apr 16, 2020, at 4:19 PM, Ken M via Histonet > wrote: > > Hello All. We have always cut all of our histology control slides at 5m. > We were told today that it is common practice to cut

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread raestask via Histonet
Should also be cut on the day they are to be stained.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Paula Keene Pierce via Histonet Date: 4/16/20 3:24 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu, Ken M Subject: Re:

Re: [Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Paula Keene Pierce via Histonet
As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing

[Histonet] Recommended thickness of Amyloid sections

2020-04-16 Thread Ken M via Histonet
Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] CAP proficiency testing

2020-04-16 Thread John Garratt via Histonet
The Canadian Pathology Quality Assurance programme runs IHC and FISH EQA internationally. We have performed PD-L1 EQA previously and we will be running a challenge January 2021. Previous EQA reports are available on line. Our website is www.cpqa.ca. We work with regularity authorities to

[Histonet] CAP proficiency testing

2020-04-16 Thread Charles Riley via Histonet
Does anyone use another vendor besides CAP to perform their PT requirements? If so, who do you use and how do you record it for inspection purposes? I am looking for a PD-L1 PT specifically but will take any other options as well as we are trying to help our budget. -- Charles Riley BS HT,

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Joseph Saby via Histonet
You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties.  Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy

Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Porter, Amy via Histonet
Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion. just thinking in a bigger box. From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020

[Histonet] FW: Microtome at home

2020-04-16 Thread Steven Crochiere via Histonet
Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet