[Histonet] Needle in a Haystack

2022-01-14 Thread Bonnie Salyer via Histonet
Do you have experience using the PELCO Biowave? Are you proficient at using TEM and specimen prep techniques for TEM? Do you have some Histology experience? What about Cryo-EM? and Immuno EM? If you can answer yes to all or most of these, you may be me my 'Needle in a Haystack' and I would love

Re: [Histonet] slide printers

2022-01-14 Thread Rathborne, Toni via Histonet
Jessica, We did this successfully. At one time, our cassette printers (Leica IPC) were not interfaced either, but we were able to use Leica's software and enter the cassette identifiers, and that information was able to be saved in the bar-code, which also printed on the cassette. Patient

Re: [Histonet] slide printers

2022-01-14 Thread P Sicurello via Histonet
Hi Jessica, Slide writers don't have to be linked to a LIS but without that you really don't save any time. All the information will be typed in manually. On the plus side-you don't have to worry about trying to read someone's hand writing if you use a slide writer. Sincerely, Paula

Re: [Histonet] microtome blades and wax

2022-01-14 Thread Rathborne, Toni via Histonet
Jessica, What has Leica said about the problem? I have always found them to be responsive and able to provide suggestions. This would be especially true with a new instrument, still under warranty. We have never had any inconsistencies with our Autocut, which was purchased last spring. Toni

Re: [Histonet] microtome blades and wax

2022-01-14 Thread P Sicurello via Histonet
Hi Jessica, Happy Friday. Do you use the same EM-400 paraffin for processing? If you use different paraffins that might contribute to your sectioning problems. If the waxes are different the tissue and the rest of the block are not one continuous unit in the "eyes" of the knife. The

[Histonet] microtome blades and wax

2022-01-14 Thread Piche, Jessica via Histonet
Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really

[Histonet] slide printers

2022-01-14 Thread Piche, Jessica via Histonet
Good Morning, Happy Friday Everyone. I was wondering what people are using/recommending for slide printers at the microtome? Do they all have to be connected to an LIS? Thank you, Jessica Jessica Piche, HT(ASCP) Histology Team Leader Waterbury Hospital Histology Laboratory