Hello all,
I have recently been using an older model MICROM HM500 M
cryostat and have been experiencing an annoying problem.
When I cut a section I like to leave the top edge of the OCT
anchored by not cutting through it all the way. This way I
can pull tension on the bottom of the section with
lol...At the end of my last post I meant to say, I DO NOT
think he knows what he is doing!
oops
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Regarding microtome blades. If you are sectioning skin, my
firm opinion is that the Thermo-Shandon MX35 Premier blade
is superb. Dermpath lab techs...you should try these out.
They are WELL WORTH using and last a lot longer than many
other types of low-profile blades.
I have used them for 10
Joe the toe Nocito...are you out there?
Joe has good ideas about nails. Maybe he will send out his
procedure again.
I like using either potassium hydroxide 10-20% or Sodium
hydroxide 10-20% for softening nail fragments before
processing.
Also, keep in mind that soft tissues attached are
I am wondering how much agitation is required in order to
achieve the clearing of paraffin from the tissue and slide.
IHC systems obviously use adhesive or + slides, aiding in
tissue adherence. Too much agitation might take take tissue
off. And what about nail fragments or hard tissues in
general?
Hello histonetters,
Does anyone know of a good source of used or economical
plastic storage drawer cabinets for paraffin blocks?
Auctions, used equipment, or otherwise retailers?
Interested in purchasing some. We have thousands of blocks
to store.
Thanks!
AB
One thing i forgot to mention wasthat when you embed, try
to orientate the
tissue so that the long axis (if there is one) lies in the
same direction as
the cutting stroke. when embedding, orientate the tissue at
a slight
diagonal, so that the knife dous not continously pass
through the tissue on
I have seen a lot of traffic on the site regarding bone
processing...If anyone is interested, I have personally
witnessed the following system being used very successfully
for the embedding and sectioning of bone, including bone
that has been surgically implanted with metal devices.
I just
Having trouble with freezing artifact in the form of tiny
fissures or cracks in mouse kidney on frozen section.
Tissue is paraformaldehyde fixed and infiltrated w 70%
aqueous sucrose OCT solution.
Anyone else seen this and know how to deal with it?
Thx
What procedures do you need to know?
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Nails
Finger or toenail sections can best be processed either
through the procedures noted by Joe Nocito on this forum (HI
JOE!) or by using a method I use (which is similar.
Soften nail fragments by placing (after fixation) them into
a solution of 20% or 10% sodium hydroxide or 20%/10%
Something I forgot to state in the procedure for nails:
heat slides in 88 degree oven for 20 mins and cool before
staining.
Andrew B.
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The point is not about gender, as I stated before...
It's about a person's health risks and lack of training
overlooked for the sake of labor. TYVM
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Kathy,
What you mentioned to me is completely inconsistent with
what I learned when I was in training.
ANYONE touching the tissue, especially taking it out of a
specimen container and transferring it to a processing
cassette, is by definition GROSSING!
Gross description (dimensions, color,
Sheesh is right, J.
CAP is all politics as far as I am concerned. It is all
about protecting the careers and paychecks of the general
pathology community.
I am thouroughly unimpressed with JCAHO, CAP et al.
If all you need to legally run a laboratory is to be CLIA
inspected, then WHY BOTHER
Correction...I meant to include High school drop-out in my
example. Furthermore, this is NOT a gender based
comment...just a real life example of some of the things I
have seen in CAP or non-CAP inspected laboratories.
Regards,
AB
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I agree that these pens are excellent.
Many years ago at the National Naval Medical center in
Bethesda, MD we had an entire run of cassettes marked with
Xylene-proof markers come out of the processor with all
the ink dissolved and NOTHING on the cassettes!
Fortunately, we had everything in order
I use nothing but SurgiPath (now LEICA) Blue Ribbon
Paraffin.
I run dermpath labs and this stuff is formulated for optimal
skin sectioning.
Highly recommend ithavent used anything else for 13
years.
AB
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If anyone is in need of a CM 1510 leica cryostat knife
holder for low profile blades, please contact me. Willing
to part with this still functional item at a reasonable
cost. FOR LOW PROFILE BLADES. Good price for back-up or
replacement for Mohs or other enterprise. Not an easy item
to find used.
...next time someone wants to paint with a broad
brushstroke and demean or belittle jr techs out there or
cast dipersions on highly-qualified medical professionals,
please remember that you, too, may be living in a glass
house.
Regards,
Andrew Burgeson
Histotechnologist
When referring to all labs in the USA being
privately-owned, I am, of course, excluding government
facilities. BUT...even those facilities employ people who
make $ working in this field and so have some interest in
the discussions.
Also, due to the fact that MEDICARE is such a big factor in
US
I think its pretty obvious that we are all talking about
o-v-e-r-u-t-i-l-i-z-a-t-i-o-n, just as the hyphen is so
overutilized in your post!
Please tell me ALL about how dermatologists overutilize.
What is the metric? How are GI and Urol and dermatology the
same? I would like to show some
well. Those wraps are available at drug stores or
grocery stores. They are cheap.
They are GREAT for tiny derm biopsies 2mm in greatest
dimension
Regards,
Andrew Burgeson
histotechnologist
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In response to a question posed some time ago, if you are
looking for a consultant to build a DERMPATH LAB, contact
Andrew Burgeson, HTL @Buckeye Dermatology inc.
Dermatopathologist/dermatologists and HISTOTECHS giving
advice and guidance, as opposed to venture capitalists and
non-medical third
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