[Histonet] HTL Career Path

2015-08-18 Thread Anna Huntley Coffey via Histonet
Hello Histonet, I have been following this listserve for a couple years now and have found it to be a very interesting and helpful forum for sharing ideas and information among individuals with varied career histories in the histopath field. I sort of fell into histology work while working on my M

[Histonet] Suggestions for staining bacteria in FFPE decaled growth plate

2016-02-26 Thread Anna Huntley Coffey via Histonet
Happy Friday, Histonetters! I have a puzzle that I could really use your expert knowledge on this morning. One of our pathologists has a decalcified FFPE mouse bone embedded longitudinally and has found some bacteria located in the growth plate. The bacteria has been confirmed with fluorescence, b

[Histonet] Samples not staining with Hematoxylin or primary

2016-07-05 Thread Anna Huntley Coffey via Histonet
Hello Histonetters, I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with ei

[Histonet] IHC antibody/assay recommendations for IL-4, IL-13, IL-4R, and IL-13Ra1

2016-07-06 Thread Anna Huntley Coffey via Histonet
Just wondering if anyone has had good luck with any of these antibodies (IL-4, IL-13, IL-4R, and IL-13Ra1) and would be willing to share vendor info and/or assay conditions they've tested? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/

[Histonet] Samples not staining with Hematoxylin or primary

2016-07-06 Thread Anna Huntley Coffey via Histonet
Thanks so much to everyone who responded! A couple people have suggested that this issue may be due to a disruption to the charge on the slide (due to high temp baking, water bath additives, etc) which causes a hydrophobic barrier to form and prevents reagent from reaching the tissue. This sounds v

[Histonet] Santa Cruz Antibodies

2016-07-18 Thread Anna Huntley Coffey via Histonet
Hello Histonet, I'm hoping someone will be able to clear something up for me. I've seen several articles in the news recently regarding the revocation of Santa Cruz' animal dealer license, the closing of their research lab, and no longer being allowed to sell antibodies past December 31 of this ye