Re: [Histonet] glass coverslippers

I have used the new Leica coverslipper. I am not a fan of this coverslipper. Too many variables with the mountant. We use what is recommended and we either have too much and it goes on top of the coverslip or not enough and we have air bubbles. They have been out to adjust and still we have

Re: [Histonet] IHC validation

Added to this.make sure you keep your work up sheets. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper

RE: [Histonet] High Complexity Testing

Could not have said it better! Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Dawson, Glen

Re: [Histonet] TTF-1 Antibody

We purchase out TTF-1 antibody from Ventana Medical and use it on their Benchmark XT stainer. I do believe they might purchase this from Cell Marque but I could be wrong. Anyway, we have no problem with this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore

Re: [Histonet] Her2 Fixation Requirement

We receive our lumpectomies and total breasts fresh. They are immediately put in 10 % formalin if no frozen is required and that time is noted on our requisition. Our processing time for formalin and the fixation time is made part of the final report. If we receive a breast biopsy specimen we

Re: [Histonet] ventilation and exposure

Our 2 closed system processors are located under a hood. We do a remote drain and fill. This is all completed under the hood. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper

Re: [Histonet] Bacterial contamination

One place you need to look is the floatation bath where you cut your slides. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper

RE: [Histonet] (no subject)

Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever

Re: [Histonet] Animal tissue processing

Caula, I have processed my fair share of animal tissue and I used the same reagents and times that I used for human tissue. With some animal organ tissue there is the possibility of drying so you need to be careful and watch out for that. You would adjust your times accordingly. I, because of

Re: [Histonet] CPT CODES FOR RE-EXCISIONS OF MELANOMA

Jim, To my knowledge the CPT code for melanomas, including re-excision, is 88305. The 88507 can be applied if it is in soft tissue or mets. Skin is usually what we deal with so we bill 88305. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital

Re: [Histonet] coverglass

We have an automated coverslipper and use coverslips from Anapath. We purchase them from STAT LAB. The item number is SL-102450. Their phone number is 1-800-442-3573. We have been very pleased with this product and have used them for many years. The price is not too bad either for the case.

Re: [Histonet] CAP Question regarding procedure manual

Victor, To my knowledge all you need is proof that the staff reviewed the manuals. We accomplish this by a sign off sheet in the front of each manual we use. The Supervisor, or designee, needs to review and sign off on each procedure in each manual. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS,

RE: [Histonet] CAP Question regarding procedure manual

When there is a change or new procedure made part of the existing manual it is noted on a separate sheet designated for this. After this new or changed procedure is signed off by the Medical Director it is given to the rest of the department to read. This is initialed by each person on that sheet.

Re: [Histonet] Workload Recording

Allison, I do keep a record of our workload but not for each individual tech in the department. I do not think that this is a requirement. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never

Re: [Histonet] Rusting in Pathology Department

Levity on Friday. Thank you. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Speak only well of people and you need never whisper Gagnon, Eric

Re: [Histonet] Tissue loss and FISH

I have to agree with Sheila's statement. When we experience this during fish or immuno staining my first thing to check is temperature and the next thing is the slides. The slides almost always is the culprit. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial

Re: [Histonet] Tracking Systems

Yes. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 rick.garnh...@mem orialhealthsystem

Re: [Histonet] Frozen Section TAT

Sue, Yes you do have your hands full. We also do multiple parts on skins, nodes, etc. etc. It is stated on the QC sheet and as part of our procedure that it is based on the initial frozen. If there is an outlier because of multiple parts that is stated as the justification for the lapse in

Re: [Histonet] Frozen Section TAT

We have a spread sheet where ALL times are written. We put the time received in the lab, the time the tech places the specimen in the cryostat, the time the case is taken to the Pathologist and the time the Pathologist signs the case out. At the end of the month I look through all of the sheets

Re: [Histonet] I have a couple of quick questions

Yes. to remove mounting media you need to leave them in the Xylene after the coverslip is removed. If the mounting media is not totally removed your stain, whatever one you are doing, will not give you the results you need. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor

Re: [Histonet] negative controls

To my knowledge for this stain to be legitimate and meet criteria you must run your positive and negative controls on the same run. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590

RE: [Histonet] negative controls

We're talking NEGATIVE controls here folks and if you use internal tissue for your positive and negative controls...they should all be processed and blocked in the same fashion as your actual patient material. We have scrutinized the negative control issue on the CAP checklist and have

Re: [Histonet] IHC FILD

I have never received any additional pay for any of my certifications. It is something I did for myself. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590

Re: [Histonet] Ventana vs Leica

Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however

Re: [Histonet] Autopsy charge

The charge is usually given to the family of the deceased. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Richard Cartun

Re: [Histonet] Recycled Formalin

I have tried to recycle both Alcohol and Xylene with not very good results. I'm not sure what kind of quality you would get on recycled formalin. You first need to know what, if anything, is being removed from the material. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor

Re: [Histonet] massons question

Bouin's is used as a mordant in this stain. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 mohamed abd el

Re: [Histonet] Breast Specimen handling

Sara, Funny that this comes up now. I attended a meeting last week and this was one of the topics. It was suggested that the tissue be stored in 70% alcohol. I personally have not done this but I am going to start doing this. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor

Re: [Histonet] block disposal

We double bag our blocks in Bio Hazard bags and then they are incinerated. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Joyce Cline

Re: [Histonet] possible AFB contaminant

My first question would be what are you using for a control? I know this sounds strange but I have, in the past, had a problem with commercially made ones. The AFB actually came away from the material and applied itself to the patient slide. Crazy huh? This caused a real mess until we figured it

Re: [Histonet] alternative for bunsen burner?

We use an alcohol burner that has a wick inside. It works well for us. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Brandi Higgins

Re: [Histonet] Microtome PM

I'm not sure where you live but we use a company called DOLBEY-JAMISON. Phone number is 610-495-2010 or 1-800-220-3073. They operate out of Pennsylvania. I have found them to be thorough and very good at their job. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore

RE: [Histonet] Responses to IHC CAP Validation question

One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity.