[Histonet] Masson's on Frozens.

Question: When we stain and H&E on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask : do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is r

[Histonet] Oil Red O

Hello all, Will Oil Red O stain phospholipids? We will be helping another lab with some work and the project out line states that Phopslipids are externalized on the cell surface during apoptosis. Can always try it I suppose. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Co

[Histonet] CD34

Been looking around a bit and seem to have it a roadblock. Does anyone know of a vendor for CD34 that will cross react with rabbit? On frozen sections, mind you. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern

[Histonet] CAP

ANP21382. What kind of policy or SOP do you all have for this question? T asks how reagents are given an expiration. This includes but is not restricted to reagents where manufacturer does not specify a date. We date made up reagents as a 6 month expiration unless we know it doesn't last that lo

[Histonet] Mycoplasma

Hello all, Will PAS stain mycoplasma? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu

Re: [Histonet] floor vibration

We have issues as HVAC is above us! Sometimes at the switch over from heat to air and vice versa the facilities personnel don't check the drains and we get a flood. Came in one morning to hear dripping and we were going to a self -eval that day.! Most of the water was in my garbage can (Thankful

[Histonet] CAP

CAP Rant: 3 months to get here and still not here. Time frame should have ended May 30th. Black out days after that. Final date actually 10th. Called before Memorial day to say they couldn't come and could they come between 1-10th June. Now narrowed to this week. Not here. I am supposed to be of

[Histonet] Fibroblasts

Hello all, Don't have a ton of time to search so am asking. Any good markers specific to fibroblasts? And am drawing a blank for regular special stains for some reason. Researcher says Vimentin is not specific enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Patholog

[Histonet] TUNEL

Hello all. Would there be any reason that slides stained with TUNEL (ISH) would not pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 minutes and then ammonia water for 1 minute. We do that rather than rinse for 15 minutes and there are usually no issues. Could a re

Re: [Histonet] sectioning issues?

You may have to call a tech at thermos to ask.. It still sounds like it it's the microtome as we know they cut. Join Histonet or the NSH facebook page and you can ask there as well. I may just be missing something obvious. histonet@lists.utsouthwestern.edu

[Histonet] P16

Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-f

[Histonet] RAt teeth

Hi all, I have bamboo rat teeth that have (ha-ha) been decalled. Obviously they are not soft. Can I use Nair on them or is there something better? I will need an H&E and an iron stain. They are not tiny samples.. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Cente

[Histonet] water under sectios

Good morning (well, in Chicago anyway) We've recently been having issues with water under the sections when picked up from the waterbath. It seems to even affect the sections during antigen retrieval after removing excess water, drying and baking I was given a block from an outside source that I

Re: [Histonet] Von Kossa staining

I use a 60 watt bulb and it works fine. Bernice -Original Message- From: John Kiernan via Histonet Sent: Wednesday, July 26, 2023 11:51 PM To: Charles Riley ; histonet@lists.utsouthwestern.edu; dsiena Subject: Re: [Histonet] Von Kossa staining Charles, A handheld light of any kind i

Re: [Histonet] preordering giemsa stains

We used to Alcian Yellow... Bernice -Original Message- From: Gudrun Lang via Histonet Sent: Thursday, February 29, 2024 8:48 AM To: 'Paula' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] preordering giemsa stains In my place it is ordered on demand, but the pathologists

[Histonet] FW: Golgi-cox staining

From: Rizaldy P Scott Sent: Wednesday, April 10, 2024 4:33 PM To: Bernice Frederick Subject: Re: [Histonet] Golgi-cox staining Hi Bernice, What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose

Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids. Bernice -Original Message- From: Colleen Forster via Histonet Sent: Friday, May 17, 2024 3:07 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Agarose embedded tissue arrays