[Histonet] autofluorescence and sudan black

Good morning!I am Carmen from the University of Valencia. Now I am performing immunofluorescence of mouse decidua. I was having problems with the autofluorescence so I found a sudan black protocol and I probed it, I incubated the tissue 30 min with sudan black after the staining, but I

[Histonet] Immune cells antibody

Hi everyone: I want to detect immune cells (natural killers) in mouse's decidua, I wonder if somebody knows a good antibody for that. thanks!!! Carmen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Problems with Frozen tissues

Good morning! I work with ovarie and mice uterus embebbed in oct. I get 8um frozen sections and put them un super frost slides, the I leave the slides in the fridge until use them. When I am going to perform the immunohistochemistry I leave the slides 1 minute at rt to atemperate the tissue,

[Histonet] Whole ovary immuno

Good morning: I want to perfom an immunohistochemistry with fluorescence in a mice whole ovary, but I am new in this and in my lab there is nobody with experience in that. If someone has some protocol that I can follow, I would appreciate it. Thank you very much kind regards from Spain

[Histonet] Thick cryosections

Good afternoon everyone! I have made 50um mice ovarian cryosections and I have used superfrost plus slides. But some of them fall of the slides when I perfomed the immuno, one colleague has told me that this is because the cuts are too thick, and I think she is rigth. I don't know if there is

[Histonet] ovarian cryosections

Hello everybody: I am here requesting your help another time ;) I want to make ovarian cryosections and I want to know if there is any problem if I leave the ovaries in 30% sucrose at 4ºC overnigth. Thank you very much. Kind regards, Carmen ___

[Histonet] DAPI question

Good morning: I am Carmen Garcia from the Faculty of Medicine of the University of Valencia, Spain. I usually use your vectashield mounting medium for fluorescence with DAPI (H-1200) and I never have any problem. But now I am working with 50um frozen sections and the problem that I have is

[Histonet] IgGs

Hello everybody: I have to carry on an experiment and I want to inject to the control mice an human IgG, someone can recommend me any? Thank you very much, Kind regards, Carmen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Paraffine-embedded tissue

Hello everyone: I am Hortensia from the University of Valencia, from Spain. I am new in this world. And I have some issues. I will apreciatte if someone with experience can help me. I am working with endometrial tissue samples, from biopses and endometrial aspirates. I want to detect the

[Histonet] HM staining

Good morning: I have a question, I want to know what can I do if I get a very strong staining... it's reversible? Thank you very much! Best regards, Carmen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Mice embryo cryocuts

Good moorning everyone: I am Carmen Garcia from the University of Valencia. I am just starting my PhD. Now I have to obtain mice embryo cryocut and I am having some problems with the morphology... We removed the embryos and put them in sucrose 30% like 5 hours, then embebbed in OCT and put

[Histonet] filopodia of Endothelial cells in corpus luteum in mouse ovary

Dear Histonet members: I am interesting in see the filopodia of the endothelial cells in the corpus luteum of mouse. I have stained 25um cryostat sections of ovary with an antibody agaisnt CD31 (PECAM) and alexa fluor and see it in the confocal microscope, but I haven't succes. someone can

[Histonet] In vitro mouse embryo culture

Good Moorning to everyone: I am Carmen Garcia a PhD student from Valencia, we are know interesting in make in vitro culture of mouse embryos to do toxicity tests and in our lab we have not experience. someone can give me some advise? or recommend me some bibliography? how long can I have the

[Histonet] Question

Good afternoon: I am Carmen Garcia a PhD student from the Faculty of medicine from the University of Valencia.We work with cryocut of mouse ovary, now I want to see the filopodia of the endothelial cells, do you have any suggestion about what I should do? Thank you so much, Carmen