I haven’t seen any answer to the question below, and I
am curious about it.
César Romero
Buenos Aires
Argentina
Message: 19
Date: Wed, 18 Feb 2015 22:31:54 +
From: James Watson jwat...@gnf.org
Subject: [Histonet] Storing antibodies in a frostless freezer
To:
I live in a Corrupt Country .
I can assure to you that this request is simply that,
Corruption. The well done job is not well paid. The one that returns money to a
corrupt person or a group of persons , yes, it is well paid.
César RomeroBuenos AiresArgentina
In the link
below ( Figure 5 ) Incomplete decalcified Bone: Basophilic and Brittle.
http://www.dentalaegis.com/cced/2011/03/alveolar-ridge-augmentation-comparion-of-two-socket-graft-materials-in-implant-cases
In the link below Complete Decalcified Bone: Pink and with well preserved
In
the past I have been consulted for lost of DAB staining in IHC when using acid
alcohol to differentiate hematoxylin.
In
my Latitude tap water is enough for bluing hematoxylin but I think that you
need something else.Trizma Base is a good bluing agent when tap water is not
good enough.Just
Another Hallmark is the Superficial Vascular Plexus of
the Skin.
You can do Muscle Actin ( HHF – 35 ) or CD 34 to
remark the vessels.
Link to a Picture
http://biologiedelapeau.fr/spip.php?page=forumid_article=21lang=en
This Superficial Vascular Plexus allows separating
Invasive Melanoma
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/
I use it with very good results.
A good trichrome stain can help very much.
Here is a link to a picture.
http://download.videohelp.com/vitualis/med/his_pic_integument2.htm
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For many years now I have used this protocol in Bone
Marrow samples.
The cortical of the bone is still a problem, but it
can be solved with longer decal time.
If you need to preserve morphology and antigenicity
for Immunohistochemistry this is the best choice.
Three Days Bouin’s Fixative (
Dear People from
Histonet
Do you still use
Mineral Oil in your Microwave Processing Protocol ?
Or you only use
ethyl alcohol ~ Isopropyl alcohol and Paraffin .
I will apreciate
any advice.
Thank you all
Cesar Romero
Buenos Aires
Argentina
I
think that what you really need is a Microwave Processor.
But
I live in a Poor World with No Machines.
I
have to report small wedge Kidney Biopsies for transplantation in two hours .
This
is my Rush Processing Protocol:
Formalin
( 1 : 10 ) 10 minutes ( they already come from the surgery
I gross
prostate needle biopsies every week.
I receive
12 samples but convert them in 6 paraffin blocks.
I stain
the Medial Samples of each level and each side with Eosin and the Lateral
Samples with EA - 36 .Example: Base Lateral Right ( EA - 36 ), with Base Medial
Right ( Eosin ).Base
The total Osmolarity of the
fixing solution should be isosmotic with tissue fluids in order to prevent
shrinkage or swelling of cells.
The best way to avoid shrinkage
is to prepare Formalin with Physiological or Isotonic Saline Solution.
The solution is 9 grams of sodium chloride ( NaCl )
I forgot to
say that the rest of the tissue processing can cause shrinkage.
If the
paraffin is too hot you will get fried tissues.
Here is a
Link with very good information about tissue processing.
http://users.adam.com.au/royellis/tp/tp.htm
I use Artist
China ( India ) Ink.
The secret
is to immerse the blocks in Bouin’s Fixative to coagulate the Ink.
After that
put the tissue blocks directly in the First Alcohol.
Don’t go
back to formalin.
Cesar Romero
Buenos Aires
Argentina
For
Manual IHC I suggest best the Sequenza Slide Rack from Thermo Scientific.
It makes your life easier and you can save money, because you only need 100 ul
of any reactive per slide.
Here is the link to the web page.
Her 2 Antibody
I do Manual IHC with Tissue Tek Slide Racks and have the same problem.
I think the problem is in the holder clip.
Only the top sample stains well.
Cesar Romero
Buenos Aires
Argentina
From: histonet-requ...@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 115, Issue 11
I think this is a great article to read about amyloid. This is the link to the
paper
http://propath.com/newsletters-immunohistochemistry-173/181-september-2004-immunohistochemistry-in-amyloidosis
It belongs to a great Pathologist Dr. Rodney T. Miller
Dr. César RomeroBuenos AiresArgentina
Hello Dear,
How are you doing these days?
You know, I bought one new pair of Nike shoes on one great online shop
www.OkIsell.com. They are so amazing!
They provide a lot of other brand new casual shoes, sport shoes, high heels,
ugg boots, such as Prada, Christian Louboutin, Gucci, Lascote,
Dear People from Histonet
Could you share your experience with C4d Antibody ?
I couldn't get any results with the mouse monoclonal from Quidel.
I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe.
I work in a Public Hospital with many kidney transplantations
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