Hi Scott,
It may be best to call CAP to get an interpretation. However, I think that
since you would be changing diluent for predictive markers, a full validation
may be required.
Best,
Dan
Daniel Pesino, HTL(ASCP)QIHCCM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes
Hi Charles - your control tissue may be autolyzed. Try using a well-processed
control tissue. If that's not it, make sure that your control tissue is being
completely deparaffinized and that it is being totally covered with the stains.
Good luck!
Daniel Pesino, HTL(ASCP)QIHC CM
Senior
rvice plan for
the ASP300S, be prepared to pay out the nose for parts. I was quoted over
$1,000 for a wax drain hose that was literally a piece of plastic piping with a
simple connector on it.
Best,
Dan
Daniel Pesino, HTL(ASCP)QIHCCM
Senior Histotechnologist
Trinity Health Of New England
Hi Ariel -
Congratulations on this big step in your career! I used the QIHC Prep Course
program by Joe Myers. The course is excellent and comes with a very helpful
resource guide. You can find it on the NSH website.
Best of luck!
Daniel Pesino, HTL(ASCP)CM QIHC CM
Senior Histotechnologist
and
knowledgeable.
Daniel Pesino, HTL(ASCP)CMQIHCCM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W 860-714-4675
114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram
-Original Message-
From: Paula via
Hi Kristy,
What platform and detection kit are you using? Would you mind sharing your DAB
protocol?
Thank you,
Daniel Pesino, HTL(ASCP)CM QIHC CM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W 860-714-4675
114 Woodland Street
Hartford, CT 06105
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We had an unsuccessful embedding run, using Embed 812 resin (an Epon
replacement, from Electron Microscopy Services). After curing in the oven, the
resin blocks came out “gummy” (flexible), perhaps due to a carryover of water
in the paper labels. Would it be possible to re-embed the tissue,
is appreciated. -- Daniel
Blackburn, Trinity College
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We have always stored fixed specimens of various sizes in plastic Nalgene
bottles, because they are so resistant to chemicals. However, we're recently
realized that the bottles grow very brittle with age, and can "explode" into
numbers shards when squeezed slightly. I think it is a result of
. This problem has made
staining and coverslipping very difficult, even with hood. Can anyone
recommend alternative solutions for (a) dewaxing prior to staining and (b)
coverslipping? thanks very much!
Daniel Blackburn
Biology, Trinity College (Hartford CT
Hello everyone,
With regards to the Technovit 9100 MMA kit, what are the required dimensions
and capacity for the chromatography column used in the destabilization step?
Also, any general tips or advice regarding the kit would be greatly appreciated.
Thank you,
Daniel Floda
She shouldn't sweat it. When I took the boards I don't recall anyone
bringing their own scope. I'm sure people do it, but it's overkill. Someone
should gently encourage her to use the microscope provided on-site. It
works the same as her BH2. I can't remember what make it was but the board
can
We were just having a debate about these charges, if I have 20 prostate
bx's can I only charge G0416 one time or 20 times?
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential
That's correct, Hazel.
Daniel Schneider, MD
Amarillo, TX
On Thu, Jan 22, 2015 at 3:14 PM, Horn, Hazel V hor...@archildrens.org
wrote:
Scenario: I am billing for immunostains and there are 3 specimens; A, B, C.
Pathologist orders 1 stain on all 3 specimens. I bill 3 88342's.
Pathologist
I have done a stink bug, spider and a few other creepy crawlers for my
kids to look at under the scope, they have no idea what they are looking
at but still love it.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended
Dear friends at HistoNet,
I'd appreciate if any of you can share a protocol in validating HPV from
Surepath Pap test vials.
I have an inquiry from a client and would appreciate your insight.
Regards,
Daniel Argüello BS, CT(ASCP)CM
Cytopathology Services Supervisor
Saint Francis Healthcare
701 N
mouse tibia.
Does anyone also have suggestions for cryotape transferring?
Thank you all.
Daniel L J Thorek, PhD.
dthor...@jhmi.edu
Division of Nuclear Medicine
Department of Radiology and Radiological Sciences
The Johns Hopkins School of Medicine
Posting for my colleague, Jeff Gordon, because his post did not go through
successfully:
Suzie, your histonet post was forwarded to me by a colleague, and I thought I
could help with your mast cell staining dilemma with pan-keratin. University
of Pennsylvania published an article a while back
I'd appreciate a copy of that processing schedule for GI bxs as well.
Thank you in advance
Sincerely,
Daniel Argüello, BS, CT (ASCP)CM
Anatomic Pathology Services Coordinator
Inspira Medical Center Woodbury
509 North Broad Street
Woodbury, NJ 08096
856-853-2030 Ext 2808
856-853-2183 Fax
arguel
for IHC...
Thanks so much for your time!
Daniel
Pirici Nicolae Daniel, MD, PhD
Department of Histology
University of Medicine and Pharmacy Craiova
Petru Rares Street 2, 200349
Craiova, Dolj
Romania
in the embedding protocol...
I hope this might help!
A HAPPY NEW YEAR TO ALL HISTONETT-ERS!
Pirici Nicolae Daniel, MD, PhD
Department of Histology
University of Medicine and Pharmacy Craiova
Petru Rares
share your processing schedule for biopsies
or any other tip that may help us resolve this issue.
Thanks in advance.
Sincerely,
Daniel Argüello, BS, CT(ASCP)CM
Anatomic Pathology Coordinator
Inspira Medical Center Woodbury
Telephone (856) 853-2030
Telephone (856) 845-0100 Ext 2808
Fax (856) 853-2183
-mail you
some sample images.
Hope this helps.
Sincerely,
Daniel Argüello, BS, CT(ASCP)CM
Anatomic Pathology Coordinator
Inspira Medical Center Woodbury
Telephone (856) 853-2030
Telephone (856) 845-0100 Ext 2808
Fax (856) 853-2183
arguel...@ihn.org
WEB: http://www.inspirahealthnetwork.org
We recently switched most of our cell blocks from agar to Histogel,
works great. We use small disposable embedding mold, put the specimen in
the bottom and add the histogel about half way up the mold, let it
harden, pop it out and put it in the cass. Also cuts much better than
agar.
Daniel Hewitt
We use a nice CIN3 on a cervical cone.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use, disclosure or distribution
I agree a lot of baby sitting with the leica, our other lab has the new
Sakura which uses glass instead of tape, they love it.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential
You can get Sharpies and highlighters in at least 7 different colors. We
color code the pathologists (Dr's Red, Blue, Pink, Orange, Purple, Green,
and Yellow.) The Sharpies and highlighters are distributed in the
appropriate departments, and the slides and paperwork get a quick mark of
color to
am trying to determine whether connective tissue has invaded muscle
territory or muscle degeneration is taking place.
I know about Heidenheins's azan trichrome, but it requires paraffin
embedding (does it?) and it would be better if I could do it on the
araldite sections.
Thanks to all, Daniel
is still covered, just a large nuisance.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use, disclosure or distribution
I don't doubt what you're saying, but can you source this info more
specifically than a consultant? I'm going to discuss this with my colleagues
and they're going to ask me.
Needless to say, a 10-20% cut in the TC of 88305, coming down the pipe in a
month or two, is a big deal.
Dan
Hi Molinari
I used microbiology long cotton wool swabs. If they are short extend them by
burning the plastic ends and joining them while they are hot. Make sure the
join is straight. Unscrew the nozzles from the machine and push the swabs in
and out to clean and open the holes. If the blockage
!
Sincerely,
Daniel
Pirici Nicolae Daniel, MD, PhD
Department of Histology
University of Medicine and Pharmacy Craiova
Petru Rares Street 2, 200349
Craiova, Dolj
Romania
P16 is now only sold through Ventana, believe they bought MTM or the
rights to P16.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim
Donadio
Sent: Monday, April 09, 2012 5:58 PM
To: Daniel Schneider
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Aetna and In-Office Lab Accreditation
To suggest that any physician who goes into private practice and has
The Wall Street Journal served up a timely article for us.
You'll see both sides of the argument below. One side is right.
DLS
HEALTH INDUSTRY
April 9, 2012, 7:22 p.m. ET
Prostate-Test Fees Challenged
By CHRISTOPHER WEAVER
Doctors in urology groups that profit from tests for prostate cancer
This is all about the money. The rest is rationalization.
The reason a group of non-pathologist physicians opens an in-house
pathology lab and hires an employee pathologist is first and foremost
to harvest profit from pathology reimbursement. Be a fly on the wall in the
partners' meetings and you
Sakura has come out with a new coverslipper which seems to work well, we
are trying it at one of our labs. In our lab we are currently using the
leica and all I can say is it has good days and really bad days, needs
lots of TLC.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email
Tried Sigma-Aldrich/Fluka?
/D
On Tue, Jan 10, 2012 at 11:02 PM, John Shelley
jshel...@sanfordburnham.org wrote:
Hi Carol,
I know that a PI here has been able to get sodium barbital from Premier
Pharmaceutical Labs. I do not have information but I am sure you can Google
it. Hope this
Why would you like to avoid detection of intracellular Abeta inclusions?
//Daniel
On Wed, Oct 26, 2011 at 8:51 AM, Hobbs, Carl carl.ho...@kcl.ac.uk wrote:
Be careful: HIER may expose not only extracellular amyloid plaques but also
intracellular APP ( or...is it intracellular A beta
Same with deionized, filtered water?
/daniel
On Thu, Jul 14, 2011 at 5:51 PM, sdys...@mirnarx.com wrote:
Hello all,
We are having an issue with water (Millipore filtered and tap is the
same problem). We put the water into glass pyrex jars (the kind with
the orange lid) and autoclave
We us a scraper I bought at Home Depot called the floor buster.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use
On 4/27/11, Nicole Tatum nic...@dlcjax.com wrote:
1st. Does anyone know if there is a rule or law that states a lab must
have a door?
I suspect that it's not explicitly spelled out, but I'd caution you,
if you build a lab without doors, you have to make provisions to
provide food and water
Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any
disposal of tissues should be checked for compatibility with
local regulations.
cheers,
Daniel
-
Daniel Prieto
Laboratorio de Cultivo de Tejidos
Departamento de Biologia Celular y Molecular
Facultad de Ciencias - Universidad de la Republica
Uruguay
El 22/09/10 12
I was wondering how any of you handle grossing of complex specimens from
multiple sites. Do you transport them all to one central grossing pool, do
them loacally and transport cassettes to a main Histology lab or some mixture
of both?
Also, if you gross at a centralized location how do deal
One question I think a lot of you are not considering is that the clinical
laboratory usually tests for analytes present in most patients. This allows
the clinical lab to more easily run validations with hundreds of patients,
using statistical tools to analyze the precision, specificity and
Just to clarify or perhaps cloud the picture a little more
Not all grossing is grossing.
So when we're talking about transferring small biopsies, in their
entirety, from a formalin container to a cassette, and describing the size,
number, and color of the tissue pieces submitted, with no
Mahoney
Omaha, NE
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Wednesday, May 19, 2010 12:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Grossing
My laboratory provides professional services to some clients and
technical services to others. The question has recently come up, who
should store blocks and slides when the pathologist and histology
laboratory are different companies?
- Dan
On Thu, Feb 11, 2010 at 8:27 AM, dkb...@chs.net wrote:
Hematology doesn't stain our
slides.
Why not?
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: 804-765-5050 l F:
804-765-5582 l dkb...@chs.net
*Daniel Schneider dlschnei...@gmail.com*
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/11/2010 09:57 AM
To
dkb...@chs.net
cc
histonet@lists.utsouthwestern.edu
Subject
Re: [Histonet] Peripheral Blood Smears
On Thu
What we have here is market distortion by a payment scheme that
doesn't reflect the real costs. You have to ask yourself why would a
urologist/dermatologist/gastroenterologist want to mess with a
histology lab?
Follow the money. Processing biopsies, I.e. the technical component
of CPT
Can you cite some authority or reference on this? I'm very interested in
the answer.
This issue came up a year or so ago in our practice. It was determined that
we couldn't charge extra for the Diff-Quik, but I'm not sure I understood
why not. I was trained to interpret all thyroid FNA's with
on
the Xpress. That said, is there a reason why we couldn't or shouldn't
process breast core Bx's on the Xpress provided they've been sitting
overnight in formalin?
Thanks!!!
Daniel Schneider
On Tue, Jul 28, 2009 at 7:20 AM, Jean Warren jwarre...@cinci.rr.com wrote:
We have the Sakura rapid tissue processor
?
Does it produce quality results on biopsies? and on surgicals? and fatty
tissue?
How does it effect IHC?
Feel free to reply on the Histonet, or, if you'd prefer, you may email me
privately.
Thank you!
Daniel Schneider, MD
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anticipate very competive compensation.
We're seeking someone with excellent communication and people skills, as
well as the requisite technical expertise.
If you're interested, or know someone who might be, please drop me an email
and we'll discuss the specifics.
Daniel Schneider, MD
an email
and we'll discuss the specifics.
Daniel Schneider, MD
On Mon, Jul 13, 2009 at 11:08 AM, Daniel Schneider dlschnei...@gmail.comwrote:
I'm a pathologist in an established group practice in Amarillo, Texas.
We're seeking a histology supervisor. Ideally, we're hiring a permanent
employee
.
The cost of a specimen container is a minute fraction of the cost of
processing a specimen. Ask yourself how much money you're really saving.
You're scrapping for pennies. Or is this some sort of green initiative?
In any case, it's asking for problems
Daniel Schneider
On Tue, Apr 21, 2009 at 11
Is there a list moderator?
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Have you asked Sakura, Leica, and Microm?
It would be interesting if they wouldn't provide manuals for their
equipment, used or not.
On Thu, Mar 26, 2009 at 11:18 AM, Jennifer Anderson
jander...@halozyme.comwrote:
Hello Everyone.
I our lab we've purchased all used equipment. None of
.
If there are any trained certified histotechs out there who would seriously
entertain relocating to West Texas for long term employment, PLEASE email me
your resume and salary requirements. If you have any questions before you
send your resume, don't hesitate to email me.
Daniel Schneider
On Wed
I would be very interested in these suggestions as well, as we would like to
improve the quality of skin embedding.
Thanks!
On Thu, Dec 11, 2008 at 10:59 AM, Jennifer Johnson
[EMAIL PROTECTED]wrote:
Can anyone suggest a really good book, atlas, etc. for embedding? The girl
that took my
A free continuing education workshop (3 CE units) is being sponsored by
Diagnostic BioSystems in cooperation with the Washington State Histotechnology
Society this Saturday, the 6th. The title is IHC Basics and Beyond,
presented by Ken Pierce. The workshop will be held at CellNetix:
in that space is scarce.
Thanks!
Daniel Schneider
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