Lisa,
We use both IHC and Warthin Starry here, depending on the Pathologist's
preference.
Glen Dawson BS, HT(ASCP) QIHC
IHC Manager
Milwaukee, WI
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
[mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:31 PM
To: Dawson, Glen; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
patient tissue being tested, how do you know if it was handled the same as the
patient tissue? For example:
1) Were they processed the same way?
Sharon,
I do a CK34BE12 P63 cocktail for basal cells charge for only one stain.
That being said, you can absolutely charge for 2 as P63 in nuclear the
CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I
am considering setting it up as a double charge in the
All,
I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there
suggest a good one?
Thank-you in Advance,
Glen Dawson BS, HT(ASCP) QIHC
IHC Manager
Milwaukee, WI
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Sheila,
Yes, IHC/ISH is considered high complexity testing. Sounds like you have a
real gem for a pathologist...sorry to hear that. Just don't let him/her pay
you like a janitor just because that's what he/she thinks that is what you are.
Glen Dawson BS, HT(ASCP) QIHC
IHC Manager
Sorry for the early morning grammar everyone...
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Tuesday, February 08, 2011 7:13 AM
To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu
Alright, if IHC is not high complexity testing, CAP should cut that massive
part of their inspection in half and concentrate more on the pathologists'
ability to accurately interpret the staining. Too much CAP regulation,
Proficiency Testing validation requirements involved if all IHC is is
All,
My docs are asking that I work up an OCT 3/4 antibody. Can anyone give me
their opinion on a good one? I'm not finding a stand out in my research so I'm
looking for some opinions from those in the trenches.
Thanx in Advance,
Glen Dawson BS, HT(ASCP), QIHC
IHC Manager
Milwaukee, WI
-Original Message-
From: Dawson, Glen
Sent: Monday, September 20, 2010 9:10 AM
To: 'Merced M Leiker'
Subject:RE: [Histonet] Posting comments
Carrie,
Until you've had some psychopath threaten you with a lawsuit, or threaten to
tell on you with your current employer
All,
I just looked at the notorious AMT cover that people are up in arms about and I
must disagree with the comments that were posted thus far. The techs here and
myself are quite surprised by the reactions because it is on a medical
catalogue and is far from being lewd or obscene. You could
I use both KP-1 and PGM-1. KP-1 is ordered about ten times as much as the
PGM-1.
Glen Dawson BS, HT QIHC (ASCP)
IHC Manager
Milwaukee, WI
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Jeff,
Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue#
RM-9119-S.
Concentration of 1:75 diluted w/Dako diluent.
Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99
degrees C, followed by a 20 min. room temp. cool down.
35 minute primary antibody
I was working at a grocery store deli with a BS in biology when a teacher from
the local technical college started there for something to do over the summer.
He told me about a program called Histotechnology that boasted 100% placement
after graduation. That was enough for me I was interning
I have had very good luck with the Surgipath Marking Pens Cat.#01880. I've
tried many they are the only ones that can stand up to my high pH Heat
Induced Epitope Retrieval (HIER).
Best of Luck,
Glen Dawson BS, HT QIHC (ASCP)
IHC Manager
Milwaukee, WI
-Original Message-
From:
All,
We as human beings are sloughing off skin cells, germs, hair, etc... Since we
could contaminate the lab, I move that all people be banned from the histo
lab...oh yeah, how would the work get done? Long story short, it is correct
that plants can cause contamination, but it is overkill to
All,
I have a request to work up a monoclonal C3d fluorescent stain (for frozen
sections). Can anyone out there suggest a good one? I am currently running
C4d clinicians are asking for the C3d as well.
Thank-you in Advance,
Glen Dawson BS, HT QIHC (ASCP)
IHC Manager
Milwaukee, WI
All,
Is anyone performing paraffin block IHC staining for IgG4? If so, a vendor
name would be much appreciated.
Thank-you,
Glen Dawson
Milwaukee, WI
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Sara,
Maybe you're looking a gift horse in the mouth. There have been many days that
I WISHED that I had no phone in my lab because it was a constant distraction in
an already hectic day. Once your boss has to hand deliver a few
messages/orders to you, he/she will most likely provide a phone
The Artisan (DAKO) is the best.
Glen Dawson BS, HT QIHC (ASCP)
IHC Manager
Milwaukee, WI
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen
arvidson
Sent: Tuesday, July 14, 2009 9:13 AM
To:
KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one
another privately. It's fast becoming like observing a daycare here.
Glen Dawson
IHC Manager
Milwaukee, WI
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All,
I vaguely remember a discussion on this, but could someone please refresh me on
who bought out Biomeda and perhaps provide contact information for whomever is
now dispensing their product?
I am looking for their FITC Streptavidin (F72) so if there are any vendors that
provide a similar
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