Sniffed my first formalin and saw first post-mortem November 1965.
Richard Edwards
Leicester U.K.
Your biggest problem is not your choice of machine but which antibodies
will work/cross react on animal tissues, many if not most, do not.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin
And what is the buffer of choice, PBS, TBS, with or without
protein/detergents or whatever, thanks.
Richard Edwards
Hi All, a Happy New Year to all, my question is, is there any point in
including iodoacetamide and phenylmethyl sulphonylchloride in the acetone(
used at -20C) used for fixing biopsies prior to GMA processing/embedding?,
many thanks.
Best technique, tinctorial or otherwise of identifying, with a view to
measuring their width, many thanks.
Richard Edwards
Hi there, does anybody know the advantage of this stain over a straight
toluidine blue stain for wbcs??, many thanks.
Richard Edwards
Leicester university
And sit around smoking as we mounted the sections from xylene, in an open
lab
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jim Burchette
Sent: 20 September 2013 14:41
To: Davis, Cassie
Cc:
-
From: O'Donnell, Bill [mailto:billodonn...@catholichealth.net]
Sent: 20 September 2013 15:09
To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center
and it was always
Hi All, I have been asked to immunostain forcomplement factors C3 and
C1q, any help/opinions gratefully received, apparently cryostat is the
preferred option, but most of our biopsies to date are in acetone-fixed
GMA processed or formalin-fixed paraffin processed, many
Hi All, I need to cost immunostaining etc., for a grant application,
formalin fixed, paraffin processing, haematoxylin and eosin,5
antibodies, 50+ biopsies, using standard ABC kit, or perhaps the Envision
package, any help or figures, or sources of figures and help gratefully
Hi All, any preferred methods?, all information gratefully received, many
thanks.
Richard Edwards
University of Leicester
U.K.
Hi All, your thoughts on the best toluidine blue method for mast cells?,
many thanks.
Richard Edwards
University of Leicester
U.K.
What total rubbish, what planet is this manager from??? Good
luck..
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore
Sent: 17 June 2013 22:11
To:
Well if you only pay peanuts you only get monkeys..
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: 09 January 2013 00:05
To: Gale Limron; histonet
Perhaps all is not lost as you will be able to see on a H@E the spaces
reluctantly vacated by the lipids.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of z o n k e d
Sent: 13 November 2012
I have seen far more damaged/unusable (apart from H@E) tissues through
under-fixation than over-fixation.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: 03
If you don't mind me butting here in Rene, most of your papers are available
in full on Pubmed.
Cheers
Richard
-Original Message-
From:
Can anyone recommend a tissue microarray system or supplier of tissue
microarray sections, many thanks.
Richard Edwards
Leicester University
We use hydrogen peroxide in a sodium azide solution,(on acetone fixed GMA
processed tissue) works fine.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon,
Loralee A
Sent: 13 July 2012 15:18
To:
???buccal..
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: 11 June 2012 15:48
To: pathr...@comcast.net; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cytology
We empty them before they get to that stage, as this would be bound to
affect the efficient working of the Coverplates.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: 06 June 2012
'
-Original Message-
From: Edwards, Richard E. r...@leicester.ac.uk
To: 'Janet Keeping' keeping.ja...@gmail.com; histonet
histonet@lists.utsouthwestern.edu
Sent: Fri, May 25, 2012 7:36 am
Subject: RE: [Histonet] certification of histotechnologists
Same in UK, I have never understood why
Same in UK, I have never understood why the separation in the Land of the
Free.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janet Keeping
Sent: 25 May 2012 12:38
To:
I have been a Trade Union(TU) member for all my working life, primarily because
I believed in the TU concept of workers coming together to further their goals
and as a counterbalance against exploitative employers, latterly I view TU
membership more as an insurance policy should I need it
Sounds to me as if you are wasting a few too many drops there!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza,
Vinnie
Sent: 19 December 2011 15:24
To: histonet@lists.utsouthwestern.edu
Yes, I met him in a bar in old Soho..
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: 09 December 2011 14:48
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet]
Looking for a supplier of the above antibody, I am aware that Pharmacia did
supply it at one time, but contacting them to date has been fruitless, many
thanks.
Cheers
Richard Edwards
Leicester
And a related question, who is distributing Pharmacia's antibodies at the
present?, many thanks.
-Original Message-
From: Edwards, Richard E.
Sent: 08 December 2011 10:47
To: Histonet
Subject: antibody to eosinophil EG2
Looking for a supplier of the above antibody, I am aware
The answer, get another supervisor, she is obviously not up to it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: 06 October 2011 00:04
To: histonet@lists.utsouthwestern.edu
Subject:
Was it decalcifying a tissue fixed in a formalin based fixative using a
hydrochloric acid containing decalcifying fluid? as even storage of HCl and
formalin in the same area is a bad plan.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
According to my source at Fisher Scientific U.K, the distributor for the
Shandon range in the U.K., the Sequenza and perhaps more importantly, the
Coverplates are still listed and available.
Richard Edwards
Well unfortunately one can't have public/governmental spending cut backs
without losing something important along the way.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sdys...@mirnarx.com
Sent: 09
If you do not employ recently trained individuals, how on earth are they
going to obtain the experience that Matt craves, it's beyond me. My daughter
recently qualified as an Occupational Therapist, when she asked for the
reason that she was unsuccessful in obtaining a post, they
In our paper on peliosis hepatis in mice we used peroxidase conjugated wheat
germ agglutinin purchased from Sigma, worked a treat.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
How about just plain old Histopathology??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: 13 June 2011 20:03
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Anatomicalalal
I
Hi Mary, I was made redundant after almost 40 years with the UK's Medical
Research Council at the age of 60 in 2008, I applied for several jobs,
was offered interviews for all of them, got the first one at the local
university, the down side is that I am on a
I am after a staining kit for the Grocott methenamine silver method for
fungi, any recommendations out there?,any opinions of the Grocott method
versus the PAS method?,many thanks.
Cheers
Richard Edwards
Has anyone ever performed a PAS on acetone fixed GMA processed tissues, we are
looking for fungi in bronchial biopsies.
Many thanks
Richard Edwards
University of Leicester
Room temperature storage has always worked fine for us, of course it all
depends on the quality of fixation and processing, and what antigen you
are after..
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Any information on current methods to counter autoflourescence in formalin
fixed paraffin processed tissues would be much appreciated.
Many thanks
Richard Edwards
Whilst value for money and costings rule then the management will
always look to save $$$s by hiring semi/unskilled
personnel.the other point in this thread is that
people are increasingly using the Histonet as an on-line and up to
date text,
Just make sure you have many many many power sockets.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen
Sent: 13 September 2010 17:29
To: Blazek, Linda; Histonet
Subject:
If the top is lumpy bumpy, take it out of the mold and simply turn it
over!.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret
Sent: 10 September 2010 15:58
To: Susan Raibley;
Many thanks to all who responded, for paraffin processed tissues the
figures suggested for the amount of shrinkage found or expected were :- more
than 5%:5-10%:10%(twice):10-15%:20%:25%:30-35%:30-40%, one
responder felt it was noticeable and another thought it was a fairy tale
Anybody aware of the degree of shrinkage in paraffin processed tissues and/or
GMA processed tissues?, many thanks.
Cheers
Richard Edwards
Leicester University.
So you could sum it up thus:- Have knife, will slice for a price.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: 08 April 2010 16:17
To: Marshall, Kimberly;
Suggest you Google Henna tattoos and then make a feature of it with a
few tasteful scrolls and patterns.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: 23 March 2010 16:00
To:
Do you mean the real McCoys??.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: 17 March 2010 15:01
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mc Coy fixative
Hi
So if one is doing an immunostaining staining run with say, 10 different
primary antibodies, at different dilutions, would you have the the same
number of different dilutions of the Biocare universal control, or just have
the one at the least dilute, say 1/10??, thanks.
Anybody out there handling/processing/sectioning mouse tissue containing mouse
prions, if so, what safety precautions do you take?.
Thanks
Richard Edwards
I cannot help you with your specific enquiry, re the microwave processor,
but gently suggest you learn from this experience and keep a comprehensive
record of methods/techniques used in your laboratory, good luck
anyway
-Original Message-
From:
Why?, and why introduce such an apparently unnecessary variable?.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Campbell
Sent: 16 December 2009 18:35
To: histonet@lists.utsouthwestern.edu
Spot on!.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: 09 December 2009 17:10
To: Josephine Garcia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Overstaining - Mayers
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