Hi Denise:I've had extensive experience with the AutoStainer line of
instruments, originally manufactured by LabVision and offered by a number IHC
product vendors. If memory serves me, the most important aspect of any
(desktop-style) PC that you intend to use as a replacement for the original
Greetings Fellow Histonetters -- I have been asked to 'post' the following
job opportunity on my employer's behalf:
Biocare Medical, a leading manufacturer of IHC/ISH reagents and
instruments, is seeking to fill the position of Immunohistochemistry Quality
Control
Manager within its
Dorothy:
I believe that the conference you're referring to is the Annual
International Retreat on Applied Immunohistochemistry and Molecular Pathology,
which
is sponsored by an organization called Pathology Learning Center.
Information relating to the meeting held this past February can
Greetings All:
For those of you who are planning to attend the National Society for
Histotechnology's Symposium/Convention later this week, I just wanted to remind
everyone that our group will hold it's only 'annual meeting' on Saturday,
9/29, from 12:00 to 12:45, (somewhere) in the
Fellow Histonetters:
Over the past few days there has been some discussion on this forum regarding
Biocare Medical's ability to continue offering the p63 antibody, some of which
has been correct and some of it incorrect. Please be assured that Biocare
still has the right to sell products that
Mesruh:
The easiest method to address such is a problem is to apply a serum-free
(e.g. casein-based) protein blocking agent, which can be obtained from a
variety of vendors. This technique is particularly effective when the reagent
is allowed to incubate with the specimen for 5 to 10 minutes
This paper can also be downloaded, indirectly, from the CAP's website:
_http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_
(http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48)
Happy (re-)validating,
Joe
--
Message: 12
Tom:
As much as I agree with your acknowledgment that its seems a bit odd for
the CAP to have a blood-banker responding to AP-related issue, I'm actually
not surprised. The folks in the 'clinical' lab have been performing more
comprehensive and complex validation procedures for a very long
Sally:
The simple answer to the question of whether or not biotin blocking is
required is ‘no’. As you stated in your inquiry, blocking need only be
performed when excessive nonspecific staining, attributable to endogenous
biotin,
is observed.
For what its worth, I think that some folks
Jason:
In my opinion, any recommendation to re-use a retrieval buffer just isn't
'good science'. The problem is that one cannot know, with any certainty,
when a solution loses it effectiveness. The primary reason that the re-use
approach has been advocated is because this device consumes a
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